Navigation Links
Cold Spring Harbor Protocols features high-throughput methods for analyzing gene activity
Date:3/3/2008

COLD SPRING HARBOR, N.Y. (Mon., March 3, 2008) New high-throughput methods are revolutionizing our understanding of transcriptional regulation. This months issue of Cold Spring Harbor Protocols (www.cshprotocols.org/TOCs/toc3_08.dtl) highlights two methods for analyzing the switches that turn genes on and off. Both methods are freely accessible on the Web site for Cold Spring Harbor Protocols (www.cshprotocols.org).

Our knowledge of epigenetics, the changes in gene regulation through modulation of chromatin not brought about by changes in the DNA sequence, has rapidly expanded in recent years. The first protocol describes a rapid, genome-wide method for identifying regions where methylation, an epigenetic control mechanism, is occurring. This method, "Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation," was developed by Marcos Estecio and Jean-Pierre Issa of the MD Anderson Cancer Center (http://www.mdanderson.org/) and Pearlly Yan and Tim Huang of the Ohio State University Comprehensive Cancer Center (http://www.jamesline.com/).

The protocol, freely accessible at http://www.cshprotocols.org/cgi/content/full/2008/4/pdb.prot4974, has proven successful for use in comparing normal tissues and tumors, helping researchers better understand the factors responsible for cancer.

The second protocol, available at http://www.cshprotocols.org/cgi/content/full/2008/4/pdb.prot4972, looks at the binding of regulatory proteins to DNA. These proteins play a role in turning activity of individual genes on and off. The method, "DNA Immunoprecipitation (DIP) for the Determination of DNA-binding Specificity," allows researchers to determine the specific DNA sequence that a regulatory protein binds. The technique allows for rapid screening of the entire genome for these binding sites, which gives insight into which genes these protein factors control.


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related medicine news :

1. mtvU & The Jed Foundation Go Beyond March Madness, Spring Break & 5th Anniversary of Iraq War, Dedicating March Programming to College Students Mental Health
2. Springer to publish International Journal of Hematology
3. Kentucky-Based Sports Medicine Duo Invited to Astros Spring Training Camp
4. Prepare to Unveil Yourself with Theraplex(R) for Spring and Summer 2008
5. Lifespring Health Sponsors the 30th Annual Napa Valley Marathon in an Effort to Promote Health and Fitness in the Northern California Community
6. Ex-Cowboys Running Back Ron Springs Named Plaintiff in Constitutional Challenge to Texas Malpractice Award Cap
7. Springer to launch new journal in emergency medicine
8. Prenatal exposure to maternal antibodies linked to autistic behaviors in offspring
9. SpringCM Improves Contract Management Efficiency for Medical Staffing Agency
10. Springer to publish new society journal in cardiology
11. Lifespring Health Sponsors the Kaiser Permanente San Francisco Half Marathon and 5K Fun Run
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/24/2017)... PHILADELPHIA (PRWEB) , ... July 24, 2017 , ... ... Health , two organizations that already work hand-in-hand on an Innovation Collaboration program, ... transform the access, quality, experience and cost of the care members and patients ...
(Date:7/24/2017)... ... July 24, 2017 , ... ... (adipose) transfer systems announces the issuance of United States Patent Number 9,695,398 (the ... adipose filtration technology. The '398 and '324 patents cover methods and systems ...
(Date:7/24/2017)... ... 24, 2017 , ... The 2017 NCSL International Workshop & ... focuses on the new ISO/IEC 17025 standards. This marks the first time in ... calibration will be changed. , As an exhibitor, METTLER TOLEDO will showcase ...
(Date:7/24/2017)... ... July 24, 2017 , ... A ... focused on delivering end-to-end sleep health care at scale, indicates record-breaking adherence ... that adherence rates for positive airway pressure (PAP) therapy, the most widely utilized ...
(Date:7/24/2017)... Calif. (PRWEB) , ... July 24, 2017 , ... A ... from food addiction to sexual abuse, and how she has overcome them. “Forbidden Memories: ... instances in her life and how she has risen above. , In “Memories,” readers ...
Breaking Medicine News(10 mins):
(Date:7/13/2017)... York City-based market research firm Kalorama Information notes seven trends ... new products to new costs, to the threat of generic ... Potential Pipeline Disruptors . Among them are ... Age-Driven Growth - True Impact Moment Arriving   ... growing population and, to a more extreme extent, the aging ...
(Date:7/11/2017)... , July 11, 2017  Bayer has awarded grants ... countries as part of its prestigious Bayer Hemophilia Awards Program ... of Philadelphia and Uniformed Services University ... are among the winners. Grant recipients were announced last night ... Hemostasis (ISTH) 2017 Congress, Berlin, Germany . ...
(Date:7/11/2017)... , July 11, 2017 Oramed Pharmaceuticals ... a clinical-stage pharmaceutical company focused on the development of ... Food and Drug Administration (FDA) has agreed to schedule ... completed Phase IIb trial of its oral insulin capsule ... Phase IIb trial met primary and secondary endpoints by ...
Breaking Medicine Technology: