Navigation Links
Clemson researchers collect and reuse enzymes while maintaining bioactivity
Date:8/8/2012

CLEMSON Clemson University researchers are collecting and harvesting enzymes while maintaining the enzyme's bioactivity. Their work, a new model system that may impact cancer research, is published in the journal Small.

Enzymes are round proteins produced by living organisms that increase the rate of chemical reactions.

"We found a robust and simple way of attracting specific enzymes, concentrating them and reusing them," said Stephen Foulger, professor in the School of Materials Science and Engineering at Clemson. "The enzymes are still functional after being harvested."

Isolating a single type of protein from a complex mixture is the most difficult aspect of the purification process. It is vital to determine the function, structure and interactions of the protein.

The researchers baited a nanoparticle to capture and recycle an enzyme. They found a way to attach an enzyme's target on the surface of a particle, allow the enzyme to bind to it, remove the particle and determine that the enzyme is still functional.

"We took a protein that was being produced in a soil and placed its food source on the outside of a nanoparticle and the protein essentially grabbed onto the food source," said Foulger. "We froze the enzyme in place and removed the particle and thus found a commercially viable way to harvest these proteins."

"This baited particle approach provides a very efficient means for isolating complex enzyme systems for use in biotechnology," said Vincent Rotello, a chemistry professor at the University of Massachusetts Amherst and leading researcher in the field. "This method also provides considerable promise for biomedical applications."

The research established a universal model for concentrating and extracting known enzyme pairings, but it can be an invaluable tool in recognizing unknown ones.

"This model is foreshadowing for what we're doing with cancer research because we're beginning to focus on the 'outside' of nanoparticles to sequester specific proteins that direct cancer cell growth," said Foulger.

The researchers' goal is to alter the cellular concentration of critical proteins in cancer to disrupt the cell's ability to spread, thereby controlling its growth in the body.


'/>"/>
Contact: Brian Mullen
mullen2@clemson.edu
864-656-2063
Clemson University
Source:Eurekalert

Related medicine news :

1. Clemson professor awarded nearly $600,000 4-year grant to study language of plants
2. Clemson researcher awarded nearly $245,000 to study automation trust and dependence
3. NIH awards $20 million over 5 years to train next generation of global health researchers
4. Researchers develop a new cell and animal model of inflammatory breast cancer
5. Researchers uncover a viable way for colorectal cancer patients to overcome drug resistance
6. Researchers Find Gene Mutations That May Be a Key to Autism
7. Researchers find evidence of banned antibiotics in poultry products
8. NJ stroke researchers report advances in spatial neglect research at AAN Conference
9. Autism by the numbers: Yale researchers examine impact of new diagnostic criteria
10. Researchers Map Brain Regions Linked to Intelligence
11. Researchers ID Genes That May Determine Mental Illness
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/24/2016)... ... 24, 2016 , ... Those who have experienced traumatic events may suffer from ... avenues, such as drug or alcohol abuse, as a coping mechanism. To avoid this ... coping following a traumatic event. , Trauma sufferers tend to feel a range of ...
(Date:6/24/2016)... ... June 24, 2016 , ... Dr. Amanda Cheng, ... treatment. Dr. Cheng has extensive experience with all areas of orthodontics, including robotic ... osteogenic orthodontics. , Micro-osteoperforation is a revolutionary adjunct to orthodontic treatment. It ...
(Date:6/24/2016)... ... June 24, 2016 , ... ... proud to recognize Dr. Barry M. Weintraub as a prominent plastic surgeon and ... women in the world, and the most handsome men, look naturally attractive. Plastic ...
(Date:6/24/2016)... ... June 24, 2016 , ... Today, MTI-GlobalStem, a provider of ... other difficult to transfect cells, announces its launch of the PluriQ™ G9™ Gene ... System is a complete system for culturing and transfecting human pluripotent stem ...
(Date:6/24/2016)... ... June 24, 2016 , ... Today, InhaleLabs.com (Inhale) offilially launched its ... by matching users with high quality water pipes within an ideal price range. The ... Inhale was founded by two brothers, Nick and Mike Hunter, who use medical cannabis ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... 23, 2016 , , , ... 7, 2016 , , , , LOCATION: , , , ... , , , EXPERT PANELISTS:  , , , Frost & Sullivan,s ... Christi Bird; Senior Industry Analyst, Divyaa Ravishankar and Unmesh Lal, Program ... global pharmaceutical industry is witnessing an exceptional era. Several new demand ...
(Date:6/23/2016)... 2016 The vast majority of dialysis patients ... Treatments are usually 3 times a week, with treatment ... travel time, equipment preparation and wait time.  This regimen ... for patients who are elderly and frail.  Many elderly ... rehabilitation centers for some duration of time. ...
(Date:6/23/2016)... 23, 2016 Leading BioSciences Inc., a ... conditions resulting from a breakdown of the mucosal ... Greg Doyle as chief executive officer. Mr. ... management team and board of directors, previously served ... He will provide continued leadership and strategic direction ...
Breaking Medicine Technology: