Navigation Links
Cincinnati Children's researchers develop first molecular test to diagnose eosinophilic esophagitis
Date:11/21/2013

Researchers at Cincinnati Children's Hospital Medical Center have developed the first molecular test to diagnose eosinophilic esophagitis (EoE), a chronic upper gastrointestinal disorder. The incidence of EoE has skyrocketed since it was first characterized two decades ago.

The test, based on a 96 gene expression profile, "offers an unprecedented opportunity to improve diagnosis and treatment, and a platform approach for other inflammatory diseases," says Marc Rothenberg, MD, PhD, director of allergy and immunology at Cincinnati Children's and senior author of the study. Up to now, gene expression profiling has not yet been well applied to inflammatory diseases, he says.

The study is published in the Dec. issue of the journal Gastroenterology. The lead author of the study is Ting Wen, PhD, a researcher in the Rothenberg lab at Cincinnati Children's.

The researchers used bioinformatics tools to develop the EoE diagnostic panel, which is composed of a 96 gene PCR array considered the most reliable tool for analyzing the expression of a focused panel of genes.

The panel was developed from a set of esophageal biopsy samples from children diagnosed with EoE and from some children who do not have EoE. The researchers applied the panel to 148 children and adults with EoE and found it identified patients with approximately 96 percent sensitivity and 98 percent specificity. The diagnostic panel was also able to distinguish patients in remission from those who did not have EoE.

The researchers have preliminary evidence that the panel could identify patients likely to have disease relapse following treatment.

"We aimed to provide a reliable next-generation diagnosis of eosinophilic esophagitis, and to offer a component of personalized medicine to each patient," says Dr. Wen. "We hope to ease the suffering of every child with EoE."

EoE is characterized by a large number of white blood cells called eosinophils in the esophagus the tube that connects the mouth to the stomach. The disease is caused by food allergies and possibly airborne allergens as well. Symptoms typically resemble gastro esophageal reflux but do not get better with anti-reflux medications. These symptoms may include trouble swallowing, vomiting, abdominal pain, and food getting stuck in the esophagus.

In an accompanying editorial, journal section editors Anson W. Lowe and Richard H. Moseley remark that "these findings provide proof of principle for the use of a tissue based molecular test in the diagnosis of EoE and highlight the advantages of such techniques over histologic analysis."

Cincinnati Children's has filed for several patents on the technology, one of which has been issued. The medical center also has entered into an option agreement with Diagnovus LLC, a specialized molecular diagnostic company focused on delivering personalized diagnostic information and services to physicians treating patients suffering from rare, orphan and less frequent diseases. The option will allow the company to validate the technology and potentially enter into a license agreement.

In addition to Dr. Rothenberg and Dr. Wen, other authors include scientists in the divisions of allergy and immunology; gastroenterology, hepatology and nutrition; and pathology.


'/>"/>

Contact: Jim Feuer
jim.feuer@cchmc.org
513-636-4656
Cincinnati Children's Hospital Medical Center
Source:Eurekalert

Related medicine news :

1. Home Care Assistance of Cincinnati Receives Home Care Pulse Certification
2. Home Care Assistance Opens New Cincinnati Office
3. Cincinnati Scientologists Kick Off School Year with an Anti-Drug Message
4. Actors, Models and Talent for Christ (AMTC) Will Hold Cincinnati Auditions for Up and Coming Talent on September 14th, 2013.
5. On September 14th, Actors, Models and Talent for Christ Will Hold Cincinnati Auditions for Up and Coming Talent of All Ages
6. NFL Season Ticket Packages: NFL Season Ticket Prices Slashed on Detroit Lions, Atlanta Falcons, Denver Broncos, New York Giants, Cincinnati Bengals, St. Louis Rams & More
7. Maroon 5 & Kelly Clarkson Ticket Prices Slashed for Concerts in Long Island, Holmdel, Cincinnati, Pittsburgh, Camden/Philly, Boston/Mansfield, Chicago, Cleveland & Detroi
8. Blake Shelton Tickets: Cheap Concert Tickets Slashes Ticket Prices in Long Island, Virginia Beach, Indianapolis, DC, St. Louis, Cincinnati, Pittsburgh, Chicago & Camden
9. Housh Inc. Recognized as One of Cincinnatis Fastest Growing Companies
10. Seva Call Launches to Put Cincinnati in Sync With Service Pros in Seconds
11. University of Cincinnati Campus Recognized Among the Greenest in America
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/24/2017)... ... March 24, 2017 , ... “The Communion of ... people of God in congregations across the United States. “The Communion of ... in 1964 who has served congregations in seven states throughout his long career ...
(Date:3/23/2017)... ... March 23, 2017 , ... Demonstrating their commitment to improving ... health departments have been awarded national accreditation through the Public Health Accreditation ... expanding network of communities across the nation whose health departments meet rigorous national ...
(Date:3/23/2017)... ... ... are unaware of the dangers that infectious bacteria play in mouth disease, while 1 out ... that dentists recommend. The ramifications of improper oral upkeep go far beyond bad breath and ... hours of work each year due to dental issues. That is why Mediaplanet is proud ...
(Date:3/23/2017)... (PRWEB) , ... March 23, 2017 , ... ... investment in Los Angeles based healthcare technology company California Healthcom Group (CHG). ... the US, and healthcare arena. With headquarters in California, CHG is a growing, ...
(Date:3/23/2017)... City (PRWEB) , ... March 23, 2017 , ... After ... fall, FaceCradle , the most-funded travel pillow in crowdfunding history, has ... delivery of its wildly popular travel innovation to Americans. , “We’re excited to be ...
Breaking Medicine News(10 mins):
(Date:3/23/2017)... -- Newborns are highly vulnerable to infections and ... young immune systems typically mount weak antibody responses. ... strong vaccine responses in newborn animals, including monkeys ... — by adding compounds known as adjuvants that ... they also describe improved adjuvant formulations that could ...
(Date:3/23/2017)... VANCOUVER , March 23, 2017 /PRNewswire/ - INVICTUS ... OTC: IVITF; FRA: 8IS) Invictus MD announces that AB ... crops in its licensed production facility under the Access ... Hamilton, Ontario . ... ACMPR in October 2016, is currently operating at half ...
(Date:3/23/2017)... On Wednesday, March 22, ... at 5,821.64, up 0.48%; the Dow Jones Industrial ... and the S&P 500 closed at 2,348.45, slightly ... out of nine sectors ended the day in ... on the following Medical Instruments & Supplies equities: ...
Breaking Medicine Technology: