Navigation Links
Chemical engineering researchers identify biofilms that cause infections
Date:6/11/2008

COLLEGE STATION June 11, 2008 Understanding the way bacterial cells "talk" to each other could lead to more effective methods for fighting the often persistent and serious infections caused by the biofilms they form, says a Texas A&M University professor of chemical engineering who not only has deciphered their language but also discovered how to quell their conversation.

Examining Escherichia coli bacteria widely considered a model organism for microbiology studies Professor Thomas K. Wood of the Artie McFerrin Department of Chemical Engineering at Texas A&M has succeeded in identifying, decoding and even modifying cell-to-cell signals so that biofilm formation is inhibited.

His findings are covered in a series of five published articles, two of which appear in The International Society for Microbial Ecology Journal a member of Nature Publishing Group's stable of scientific publications. In addition, his progress is detailed in The Public Library of Science ONE, Applied and Environmental Engineering and in BMC Microbiology.

Wood's work is important in addressing the widespread health issues resulting from bacteria in its biofilm form. Put simply, biofilm is a protective and adhesive slime excreted by bacteria that have joined together to form a community. The substance can grow on a variety of living and nonliving surfaces, including submerged rocks, food, teeth (as plaque) and biomedical implants such as knee and hip replacements.

And where there's an infection, there is usually biofilm.

The National Institutes of Health, Wood noted, estimate that about 90 percent of infections in humans are caused by biofilm. The Centers for Disease Control estimate biofilm to be present in 65 percent of hospital-acquired (nosocomial) infections. Biofilms have been linked to everything from gum diseases to cystic fibrosis. They typically are the cause for the fatal infections that develop post surgery. More commonly, biofilm is the chief culprit behind the nagging ear infections so common among children.

"It's been only in the last few years that pediatricians have realized that children are crying day-in and day-out from ear infections because it's a bacterial biofilm on their eardrums," Wood said. "Prior to that, the approach was to treat the infections with drugs that got rid of bacteria but not bacteria in a biofilm, and it was completely ineffective.

"When bacteria are growing within a biofilm, that growth takes place in a different way than when bacteria are swimming freely in suspension. Pharmaceutical firms make antibiotics to kill bacteria in suspension. Those are 1,000 times less effective on a biofilm. It's only in the last 10 years that we've realized people have died not because of free-floating bacteria but because of bacteria in a biofilm."

Wood's efforts to mitigate biofilm formation began with the recognition that construction of this substance was anything but random. To the contrary, biofilm formation is an extremely ordered process a fact affirmed by examining biofilm structure on a microscopic level.

Though it appears as slime to the naked eye, biofilm is actually composed of several hills and valleys of varying heights and depths. These structural differences allow for nutrients to make their way to all bacteria within the biofilm community, Wood said. Despite the numerous formations present, these pillars and plateaus seldom collide with each other. The reason for that, Wood said, is that each bacterial cell is able to "talk" to one another and signal its location so that neighboring cells do not begin construction in an occupied space. If that happened, the bottom layers of the community could be sealed off from the nutrients on which they depend, Wood explained.

In-depth examination of those critical signals by Wood and his team of researchers found that E. coli relies on a compound known as autoinducer-2 (AI-2) for biofilm formation. Each E. coli bacterium produces this compound and then releases it in the cell's external environment. Eventually a large amount of AI-2 becomes present outside of the cells, and through a process called quorum sensing each bacterium re-absorbs the AI-2 when a specific concentration has been achieved, Wood explained. When the AI-2 re-enters the cells it activates an entirely new set of behaviors for the bacterium in this case, signaling when and how to begin building a biofilm.

"What we're doing is examining how AI-2 is prompting the cell to make more biofilm," Wood said. "Sugars are the mortar. We identified the specific type of mortar the sugar known as colonic acid. We found that AI-2 helps E. coli produce more biofilm by making colonic acid, which is a kind of sugar. If you can understand how a biofilm is formed, then you can start to attack it at different stages."

And that's exactly what Wood has done.

Further research by Wood revealed that E. coli uses two different signals to control biofilm formation. Which signal is utilized depends on the temperature of the external environment, Wood said. In other words, these bacteria change their "language" if they are inside the body versus outside the body. Whereas AI-2 is the signal utilized by E. coli inside the body, Wood discovered that a compound known as indole is used for biofilm growth outside of the body for instance on surgical replacement parts yet to be implanted.

"The second signal that we unraveled is indole a derivative of amino acids," Wood said. "Amino acids are what proteins are made out of. All living things need to make amino acids. All living things make this specific amino acid called tryptophan. It turns outs E. coli converts tryptophan into indole."

Armed with that knowledge, Wood discovered a method for inhibiting biofilm formation by modifying the indole signal in a way that would confuse the bacteria. In lay terms, think of Wood's achievement as adding a few extra letters to a spoken word. The resulting gibberish is not understood by other bacterial cells in the community, and the massive construction project never gets off the ground.

"Once we realized that indole was the signal, then we could slightly modify indole by putting an OH [hydroxide] molecule on it." Wood said. "The new compound is called 7-hydroxyindole. We also found that a different type of modification would not be successful. We learned that you can trick bacteria, but you have to do it well, and another modification does not have any effect on stopping biofilm formation.

"By adding that hydroxyl group and making the 7-hydroxyindole, we turn off the bacterium's ability to talk. We short-circuit the bacterium, and it becomes less of a bad actor."


'/>"/>

Contact: Thomas K. Wood
thomas.wood@che.tamu.edu
979-862-1588
Texas A&M University
Source:Eurekalert

Related medicine news :

1. 8th International Conference on Pain and Chemical Dependency
2. American Chemical Societys Weekly PressPac -- June 4, 2008
3. Prenatal biochemical screening only detects half of chromosomal abnormalities
4. Cornell receives federal grants to create fabrics to render toxic chemicals harmless
5. American Chemical Societys Weekly PressPac -- May 21, 2008
6. Major missed biochemical pathway emerges as important in virtually all cells
7. Study finds that recalled Aqua Dots did contain poisonous chemical
8. BASF Podcast: The Chemical Reporter - Why do You Get Garlic Breath?
9. Safe, Green Insecticides Can Reduce Chemical Exposure in Homes and Gardens
10. New chemical can kill latent tuberculosis bacteria
11. Rodent study finds artificial butter chemical harmful to lungs
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/2/2016)... ... December 02, 2016 , ... Mediaplanet’s ... ostomy patients, standing as living proof that attitude and determination can combine into ... issues that spike around the holidays. This campaign will offer patients a new-found ...
(Date:12/2/2016)... ... December 02, 2016 , ... CloudLIMS.com, a class-leading provider ... CloudLIMS Lite. CloudLIMS Lite helps biobanks, clinical, research and testing laboratories keep track ... The new version is a faster and a more efficient product, allowing batch ...
(Date:12/2/2016)... PA (PRWEB) , ... December 02, 2016 , ... Rijuven ... stress and overall health, day and night. No other wearable health technology on the ... We wanted to give poeple more meaningful insights about their health than the ...
(Date:12/2/2016)... ... December 02, 2016 , ... On ... individuals and families from eight different sites throughout Miami-Dade and Broward counties. This ... volunteers worked very hard on Thanksgiving morning by putting together individual meals via ...
(Date:11/30/2016)... ... November 30, 2016 , ... ... we have been designated as a Cigna Infertility Center of Excellence. The Cigna ... standards. , “It’s an honor to be designated a Cigna Infertility Center ...
Breaking Medicine News(10 mins):
(Date:12/2/2016)... Research and Markets has announced the addition of the ... to 2022" report to their offering. ... , , The global ultrasound ... is expected to grow at a CAGR of 5.5% during 2016-2022. Based ... during the forecast period, a CAGR of 8.8% in the global market. ...
(Date:12/2/2016)... ATLANTA , Dec. 2, 2016 Quantum ... screening and expert radiologist interpretation directly to women at ... partners with corporations, such as Delta Air Lines and ... cancer screening as a component of wellness initiatives. ... women of SunTrust. It enables them to have a ...
(Date:12/2/2016)... -- In the first ever attempt to include phytocannabinoids ... C. sativa, the Hebrew University in Jerusalem, the University ... , the Universita` del Piemonte Orientale and Phytoplant Research ... unified inventory of phytocannabinoids of different botanical origin. ... chemical and structural diversity of phytocannabinoids. As a result ...
Breaking Medicine Technology: