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Chemical can reproduce complications for some patients
Date:4/30/2009

thetized, tracheotomized, ventilated using a rodent ventilator, and then instrumented so that the researchers could measure cardiac output, heart rate, stroke volume, blood pressure, and heart contractile function. Measurements were made at baseline and again 60 minutes after infusion of CHX or saline.

Protocol 2 was designed to measure cardiovascular autonomic function, including how well the nervous system was able to maintain blood pressure in the face of a challenge. After the animals were anesthetized and instrumented to measure blood pressure, both carotid arteries were occluded (obstructed), which stimulates a reflex called the baroreflex that controls blood pressure. After this baseline assessment of reflex function, CHX or saline was infused and allowed to circulate for 30-40 minutes, followed by another round of occlusions. A subsequent identical infusion was given and allowed to circulate for an additional 30-40 minutes, followed by a final round of occlusions.

Protocol 3 was designed to measure edema formation, or the volume of water that has been retained in major organs of the body. After the animals were anesthetized, each rat received an infusion of either CHX or saline, which was allowed to circulate through the body for 2 hours. After 2 hours, the animal was euthanized, and the liver, kidneys, lungs, and skin were harvested, weighed, dried at 100C, and weighed again to quantify the fluid content in each organ at the time of death. Results In protocol 1, all baseline values were similar between saline and CHX groups. In the 60 minutes following saline infusion, none of the variables changed significantly from baseline. The CHX infusion, however, induced significant adverse changes in all cardiovascular variables. Both stroke volume and heart rates values were significantly depressed after one hour of CHX, resulting in a significant decline in cardiac output in the CHX rats. Stroke volume, in turn, was depressed beca
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Contact: Donna Krupa
DKrupa@the-aps.org
301-634-7209
American Physiological Society
Source:Eurekalert

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