Navigation Links
Changes in hyaluronan metabolism key in adaptation of keratinocytes to radiation injury
Date:7/2/2013

As the outermost layer of skin, epidermis is crucial in forming a permeability barrier and protection against various environmental agents. Thus, investigating the biology of its most important cell type, the keratinocyte, is key to understanding the effects of solar ultraviolet radiation in skin, and helps design effective means of protection against excessive exposure. It has already previously been shown with both cell culture and in vivo animal models that UV irradiation increases the expression of hyaluronan, which is an important carbohydrate of the extracellular matrix. Hyaluronan is also clearly increased in tissue sections from early stage squamous cell carcinomas, one important risk factor of which is exposure to UV radiation.

Hyaluronan metabolism in cells and tissues has long been a special area of interest in the research group of Professors Raija and Markku Tammi at the Institute of Biomedicine, University of Eastern Finland. One important target tissue and focus in these studies has been the skin. In a recent paper published in The Journal of Biological Chemistry, the group demonstrated that already fairly low doses of UVB activate the accumulation and degradation of hyaluronan. This occurs in both simple monolayer cultures of keratinocytes and a stratified, three-dimensional model that mimics normal skin epidermis. As intracellular signalling pathways are activated, the hyaluronan producing enzymes (Has1-3) and hyaluronan synthesis are upregulated. Particularly, it was shown that Has1 and Has2 are regulated by the MAP kinase p38, and Has3 by the calcium-dependent protein kinase CaMKII.

Since hyaluronan content and the absolute and relative quantities and activity of the hyaluronan synthases depend on the tissue context and physiological conditions, it's crucial to understand the underlying regulatory mechanisms. In this study, the significantly upregulated hyaluronan metabolism in the keratinocytes seemed to be dependent specifically on the Has3 enzyme. Blocking its expression with a specific siRNA reversed the UVB-induced hyaluronan production nearly to the level of the control cells. The results thus strongly indicate that epidermal keratinocytes exposed to UVB specifically activate their hyaluronan synthesis by regulating the HAS-enzymes via at least two different signalling pathways. This may be one mode for the cells to adapt to radiation damage.

Even though more research is warranted, the new data further our understanding about the significance of hyaluronan for keratinocyte function under environmental stress. This activation of hyaluronan metabolism after excessive UVB exposure may also prove to be a target for regulation when trying to control and understand tissue malignancy and pre-cancerous alterations.


'/>"/>

Contact: Leena Rauhala
leena.rauhala@uef.fi
358-503-871-326
University of Eastern Finland
Source:Eurekalert

Related medicine news :

1. Teen Drinking May Boost Odds of Precancerous Breast Changes
2. Changes in gene expression may help explain high blood pressure in pregnancy
3. Brain Changes May Hamper Decision-Making in Old Age
4. Specific protein triggers changes in neurons in brain reward center linked to cocaine addiction
5. Molecular probes identify changes in fibronectin that may lead to disease
6. Dietary changes help some children with ADHD
7. Palliative care resource helps ease changes
8. Chronic cocaine use triggers changes in brains neuron structure
9. Eye Changes May Predict Heart Disease in Blacks With Diabetes
10. From One Generation to the Next, Dental Care Changes
11. Tiny Tots in the Dentists Chair Among Changes in Pediatric Dentistry
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... PLAINSBORO, N.J. (PRWEB) , ... June 27, 2016 , ... ... same sources, yet in many ways they remain in the eye of the beholder, ... Oncology (EBO), a publication of The American Journal of Managed Care. For the full ...
(Date:6/26/2016)... Birmingham, Lake Orion, Clarkston, Michigan (PRWEB) , ... ... ... their direction with respect to fertility once they have been diagnosed with endometriosis. ... for tolerable intercourse but they also require a comprehensive approach that can help ...
(Date:6/25/2016)... ... June 25, 2016 , ... First Choice Emergency Room , the largest ... as the Medical Director of its new Mesquite-Samuell Farm facility. , “We are ... Mesquite location,” said Dr. James M. Muzzarelli, Executive Medical Director of First Choice Emergency ...
(Date:6/24/2016)... ... ... A recent article published June 14 on E Online details ... to state that individuals are now more comfortable seeking to undergo not only the ... and cheek reduction. The Los Angeles area medical group, Beverly Hills Physicians (BHP) notes ...
(Date:6/24/2016)... ... 2016 , ... Global law firm Greenberg Traurig, P.A. announced that 20 Florida ... their peers for this recognition are considered among the top 2 percent of lawyers ... as members of this year’s Legal Elite Hall of Fame: Miami Shareholders Mark ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... 24, 2016  Consumers have taken a more ... placed more emphasis on patient outcomes. ... in the pharmaceutical industry have evolved beyond just ... companies are focusing on becoming more patient-oriented across ... and services that improve health. ...
(Date:6/24/2016)... June 24, 2016 Research ... "Structural Electronics 2015-2025: Applications, Technologies, Forecasts" ... In-Mold Electronics, Smart Skin, Structural Health ... Structural electronics involves electronic and/or electrical ... structures, replacing dumb structures such as vehicle bodies ...
(Date:6/24/2016)... , June 24, 2016 ... Market by Type (Standard Pen Needles, Safety Pen Needles), ... (Insulin, GLP-1, Growth Hormone), Mode of Purchase (Retail, Non-Retail) ... MarketsandMarkets, This report studies the market for the forecast ... to reach USD 2.81 Billion by 2021 from USD ...
Breaking Medicine Technology: