Navigation Links
Cell growth technology promises more successful drug development
Date:9/18/2007

Scientists have developed unique technology to grow stem cells and other tissue in the laboratory in conditions similar to the way they grow in the human body.

The technology, developed and patented by scientists at Durham University and its spin-out company ReInnervate Limited, is a plastic scaffold which allows cells to be grown in a more realistic three-dimensional (3D) form compared to the traditional flat surface of a Petri dish.

Evidence gathered by the research team shows that the technology is a cheap and straightforward way of cultivating cells in 3D. Using it could lead to more successful drug development programmes and a reduction in unnecessary tests on animals.

A study proving the effectiveness of the scaffold, funded by ReInnervate and the Engineering and Physical Sciences Research Council (EPSRC), is published today in the Journal of Anatomy.

A large proportion of drugs fail at the testing stage, costing industry millions of pounds in research and development costs and failed drugs trials every year. At the moment, most drugs in development are first tested on cells grown in two-dimensions (2D) in standard laboratory equipment such as Petri dishes or flasks but cells in the human body form tissues and grow in more complex, three-dimensional ways.

The new study tested the toxic effect of a cancer drug called Methotrexate (MTX) on liver cells grown in three and two dimensions. Liver cells are frequently used in the drug development industry to test the toxicity of drugs and MTX is known to cause liver damage at high doses.

Tests showed that the structure and properties of the cells grown using the 3D scaffold were most similar to liver cells found in the human body, compared with the 2D cells which appeared disorganised when viewed under the microscope.

When subject to doses of MTX, cells grown in 2D died at very low concentrations, whereas 3D cells grown using the scaffold were far more robust and more accurately reflected the behaviour of cells in the human body when subjected to similar doses of the drug.

Dr Stefan Przyborski, a senior researcher with Durham University and Chief Scientific Officer of ReInnervate, has tested ten different tissue types on the scaffold, including bone, liver, fat and stem cells from bone marrow, and is marketing the product for commercial use.

The scaffold is made of highly porous polystyrene, is about the size of a ten pence piece and resembles a thin white disc. It has a structure resembling that of a sponge and is riddled with tiny holes which scientists are able to populate with cells which are then cultivated under laboratory conditions.

The technology has potential to be used to grow human stem cells for drug development. Their use may reduce the need for the tests on animals that are usually the next step before progressing to clinical trials in humans.

Another current use of the scaffold involves growing skin cells which are being used by the cosmetics industry to test cosmetics.

Dr Przyborski said: Our results suggest that testing drugs on liver cells using our 3D culture system may be more likely to reflect true physiological responses to toxic substances. Because the 3D cells are cultivated under more realistic conditions, it means that they function more like real tissues.

Scientists are therefore able to gain a more accurate idea of how a drug will behave in the human body, knowledge which can contribute to improving the efficiency of drug discovery, reducing drug development costs, and may help reduce the number of animals in research.

There are other ways to growing cells in 3D in the laboratory. However, these approaches are restricted by their variability, complexity, expense and they are not easily adapted to routine use in high throughput screening studies.

Our technology is essentially a well engineered piece of plastic that provides a suitable environment for cells to grow more naturally in a 3D configuration. Our product is available off-the-shelf, it is easy to use in routine applications, it is highly adaptable to different tests, it is inert and it is cheap and easy to produce and manufacture.

Dr Stefan Przyborski and colleagues at Durham University play a key role in the North-east England Stem Cell Institute (NESCI), a unique interdisciplinary collaboration to convert stem cell research and technologies into cost-effective, ethically-robust 21st century health solutions to ameliorate degenerative diseases, the effects of ageing and serious injury.


'/>"/>

Contact: Dr. Stefan Przyborski
stefan.przyborski@durham.ac.uk
44-191-334-1341
Durham University
Source:Eurekalert

Related medicine news :

1. More The Usage More The Growth In The Adult Brain.
2. A new growth factor identified
3. Anthrax drug doxycycline could stunt fetal growth
4. Anti-Cancer Drug Restores Normal Cell Growth
5. Zinc may enhance growth in Young sickle cell patients
6. Drug stabilizes cancer growth
7. Enzyme stimulates growth of adult stem cells
8. Mental growth in babies at risk
9. Low-dose Growth Hormone (GH) with diet and exercise may help weight loss
10. New Molecule that can stop the growth of Viruses
11. Human Growth Hormone Found To be Dangerous For Human Use
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/12/2016)... ... February 12, 2016 , ... Healthcare careers in the medical laboratory, nursing, and ... the website of healthcare staffing leader Aureus Medical Group during the month of January. ... travel therapy positions and in travel and direct hire opportunities in other allied ...
(Date:2/12/2016)... ... ... Yisrayl Hawkins, Pastor and Overseer at The House of Yahweh in Abilene, Texas, ... real facts surrounding all those Bible stories. For generations families have gathered to hear ... says there is more to these than just mere “stories”. , The article ...
(Date:2/12/2016)... ... 12, 2016 , ... With the exception of restorative dentistry, to date there has been no ... recent approval by the FDA, there is a now a new protocol in stopping cavity ... very simple and quick to apply. The application is as simple as drying the ...
(Date:2/11/2016)... ... February 11, 2016 , ... “ HEALING MIND : ... a Well Managed Mind” (published by Balboa Press) teaches readers how to become their ... through profound love, author Janice McDermott, M.Ed., LCSW, offers an understanding of how to ...
(Date:2/11/2016)... ... February 11, 2016 , ... Student-doctors ... Osteopathic Association’s (AOA) Match Program Tuesday, February 9, taking one of the final ... osteopathic graduate medical education positions across the country. Of the 103 student-doctors who ...
Breaking Medicine News(10 mins):
(Date:2/11/2016)... , February 11, 2016 F ... answers at the ... a world-leading provider of scientific, technical and medical information products and services, ... to evidence-based, peer reviewed clinical information via a mobile device. Elsevier designed ... ClinicalKey for Nursing. The new app is available in Android ...
(Date:2/11/2016)... spurred by a major "team investment" by Bruce Montgomery , one of this area,s most ... to CEO Leen Kawas , PhD. Photo ... ... ... Kawas said the round was intended to be ...
(Date:2/11/2016)... 2016  AcelRx Pharmaceuticals, Inc. (NASDAQ: ACRX ) ... at the 38th annual John A. Boswick , ... is being held February 14-18, 2016 in ... advancements in wound healing, burn care, and infection control, ... Zealand Burns Association, Academy of Physicians in Wound Care ...
Breaking Medicine Technology: