Navigation Links
Cancer stem cells generated by cancer outgrowth
Date:4/2/2009

Scientists have discovered that growing mouse skin cells in spheres can lead to generation of cells with properties of cancer stem cells, even without genetic manipulation of stem cell genes. This unexpected finding, published by Cell Press in the April 3rd issue of the journal Cell Stem Cell, provides a potential pathway for generation of cancer stem cells from differentiated cells and may even eventually lead to safer strategies for creation of induced pluripotent stem cells for use in regenerative therapies.

"A hallmark of all solid tumors is the outgrowth of cancer cells into three-dimensional structures," explains senior study author, Dr. Douglas C. Dean, from the University of Louisville Health Sciences Center in Louisville, Kentucky. Dr. Dean and colleagues examined whether abnormal cell configurations might trigger reprogramming of differentiated cells into cells that resembled cancer stem cells.

The researchers observed that mutation of all of the retinoblastoma tumor suppressor gene (RB1) family members, known to be critical for regulating cell-contact inhibition and restricting growth of normal cells into three-dimensional tumor-like structures, led to an outgrowth of cells into spheres that triggered generation of cells similar to cancer stem cells. Surprisingly, the cancer stem cell-like cells expressed key genes expressed in embryonic stem cells and gave rise to a variety of differentiated cells.

Interestingly, cells with only one RB1 mutation remained contact inhibited, but when mechanically scraped off the dish and forced to form spheres, they also exhibited cancer stem-like characteristics. Even cells with intact RB1 genes could be forced to form spheres, suggesting that the reprogramming did not require the loss of RB1. The researchers went on to show that the cancer stem-like cells isolated from the spheres with disrupted RB1 genes formed tumors when injected into mice and differentiated into mature cells in advancing cancers.

These results using cultured cells lead the authors to hypothesize that cancer stem cells may be generated as a direct function of the outgrowth of cells in the animal. "To our knowledge, this is the first example that silenced endogenous embryonic stem cell genes can be spontaneously reactivated in differentiated cells," says Dr. Dean. "We propose that the loss of cell contact inhibition when the RB1 pathway is inhibited leads to outgrowth into sphere-like structures, and these conditions in the advancing cancer trigger reprogramming of differentiated cells to cells with properties of cancer stem cells."


'/>"/>

Contact: Cathleen Genova
cgenova@cell.com
617-397-2802
Cell Press
Source:Eurekalert

Related medicine news :

1. Conventional prognostic factors fail to explain better prostate cancer survival in most Asian men
2. Survival differences by race most apparent in advanced stages of breast cancer
3. MRI finds breast cancer before it becomes dangerous
4. Investigators uncover intriguing clues to why persistent acid reflux sometimes turns into cancer
5. Pathway links inflammation, angiogenesis and breast cancer
6. Radiologists encouraged to look beyond cancer for clinically unseen diseases
7. Diet high in meat, fat and refined grains linked to risk for colon cancer recurrence, death
8. Immune deficiency linked to a type of eye cancer
9. Drop in breast cancer incidence linked to hormone use, not mammograms
10. Breast cancer prevention practices vary across Canada
11. First biomarker discovered that predicts prostate cancer outcome
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/12/2017)... (PRWEB) , ... October 12, 2017 , ... Planet Fitness, ... the U.S., announced today its plans to open a flagship location in Covington, LA ... the former Rooms To Go store next to Office Depot in the Holiday Square ...
(Date:10/12/2017)... ... 2017 , ... IsoComforter, Inc. ( https://isocomforter.com ), one of the ... new design of the shoulder pad. The shoulder pad provides optimal support and ... pain while using cold therapy. By utilizing ice and water that is circulated from ...
(Date:10/12/2017)... ... October 12, 2017 , ... ... in post-acute health care, have expanded their existing home health joint venture through ... AccentCare has been operating a joint venture home health company with Asante, delivering ...
(Date:10/12/2017)... ... October 12, 2017 , ... Dr. Parsa ... contributed a medical article to the newly revamped Cosmetic Town journal ... the hair transplant procedure known as Follicular Unit Extraction (FUE). , ...
(Date:10/12/2017)... Washington, D.C. (PRWEB) , ... October 12, 2017 , ... ... of their peers in Washington, D.C., for the 49th Congress of the International ... Ph.D ., Vice President of the Center for Cancer and Blood Disorders ...
Breaking Medicine News(10 mins):
(Date:10/11/2017)... 11, 2017  BioPharmX Corporation (NYSE MKT: BPMX) researchers ... an innovative way to use nonlinear optical imaging to ... new drugs. ... will show how researchers from BioPharmX and the Wellman ... used a suite of imaging techniques in what is ...
(Date:10/10/2017)... Oct. 10, 2017   West Pharmaceutical Services, Inc. ... for injectable drug administration, today shared the results of ... for improving the intradermal administration of polio vaccines. The ... Summit in May 2017 by Dr. Ondrej Mach ... World Health Organization (WHO), and recently published in the ...
(Date:10/4/2017)... 2017 OBP Medical , a ... devices, today announced regulatory approval from ... Agência Nacional de Vigilância Sanitária (ANVISA)) to market ... retractor with integrated LED light source and smoke ... exposure of a tissue pocket or cavity during ...
Breaking Medicine Technology: