Navigation Links
Building a biochemistry lab on a chip
Date:2/12/2013

Miniaturized laboratory-on-chip systems promise rapid, sensitive, and multiplexed detection of biological samples for medical diagnostics, drug discovery, and high-throughput screening. Using micro-fabrication techniques and incorporating a unique design of transistor-based heating, researchers at the University of Illinois at Urbana-Champaign are further advancing the use of silicon transistor and electronics into chemistry and biology for point-of-care diagnostics.

Lab-on-a-chip technologies are attractive as they require fewer reagents, have lower detection limits, allow for parallel analyses, and can have a smaller footprint.

"Integration of various laboratory functions onto microchips has been intensely studied for many years," explained Rashid Bashir, an Abel Bliss Professor of electrical and computer engineering and of bioengineering at Illinois. "Further advances of these technologies require the ability to integrate additional elements, such as the miniaturized heating element, and the ability to integrate heating elements in a massively parallel format compatible with silicon technology.

"In this work, we demonstrated that we can heat nanoliter volume droplets, individually and in an array, using VLSI silicon based devices, up to temperatures that make it interesting to do various biochemical reactions within these droplets."

"Our method positions droplets on an array of individual silicon microwave heaters on chip to precisely control the temperature of droplets-in-air, allowing us to perform biochemical reactions, including DNA melting and detection of single base mismatches," said Eric Salm, first author of the paper, "Ultralocalized thermal reactions in subnanoliter droplets-in-air," published in the Proceedings of the National Academy of Sciences (PNAS) on February 12.

According to Salm, approaches to perform localized heating of these individual subnanoliter droplets can allow for new applications that require parallel, time-, and space multiplex reactions on a single integrated circuit. Within miniaturized laboratory-on-chips, static and dynamic droplets of fluids in different immiscible media have been used as individual vessels to perform biochemical reactions and confine the products.

"This technology makes it possible to do cell lysing and nucleic acid amplification reactions within these individual droplets the droplets are the reaction vessels or cuvettes that can be individually heated," Salm added.

"We also demonstrate that ssDNA probe molecules can be placed on heaters in solution, dried, and then rehydrated by ssDNA target molecules in droplets for hybridization and detection," said Bashir, who is director of the Micro and Nanotechnology Laboratory at Illinois. "This platform enables many applications in droplets including hybridization of low copy number DNA molecules, lysing of single cells, interrogation of ligandreceptor interactions, and rapid temperature cycling for amplification of DNA molecules.

"Notably," Bashir added, "our miniaturized heater could also function as dual heater/sensor elements, as these silicon-on-insulator nanowire or nanoribbon structures have been used to detect DNA, proteins, pH, and pyrophosphates.

By using microfabrication techniques and incorporating the unique design of transistor-based heating with individual reaction volumes, 'laboratory-on-a-chip' technologies can be scaled down to 'laboratory-on-a-transistor' technologies as sensor/heater hybrids that could be used for point-of-care diagnostics."


'/>"/>

Contact: Rashid Bashir
rbashir@illinois.edu
217-333-3097
University of Illinois College of Engineering
Source:Eurekalert

Related medicine news :

1. American Association of Radon Scientists and Technologists (AARST) Releases Nation's First Standard for Mitigating Radon (Radioactivity) in Multifamily Buildings
2. Visual Impact Muscle Building Review Revealed Insight View Of Muscle Enlargement By Rusty Moore In His Program Visual Impact Muscle Building
3. Burn the Fat, Feed the Muscle: Review Examining Tom Venuto’s Training Program Released by QuickMuscleBuildingTips.com
4. Netmark Divulges Their Link Building Strategies
5. Hyper Gain: Review Examining ASR's Creatine Supplement Released By QuickMuscleBuildingTips.com
6. Visual Impact Muscle Building: Review Examining Rusty Moore's Training Program Released By DietsAndFitnessGuides.com
7. Rebuilding blood vessels through gene therapy
8. Steering stem cells to become 2 different building blocks for new blood vessels
9. Dental Marketing Formula.com has been Featured in The LA Daily News for their Specialized Practice Building Solutions in the Dental Industry
10. UC research explores relationship-building program for male same-sex couples
11. Rebuilding a whole heart for children born with only half of 1
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... ... 2016 , ... Brent Kasmer, a legally blind and certified personal trainer is helping to develop ... The fitness app plans to fix the two major problems leading the fitness industry today:, ... all type program , They don’t eliminate all the reasons people quit their ...
(Date:6/25/2016)... Austin, TX (PRWEB) , ... June 25, 2016 , ... ... Fellow of the American College of Mohs Surgery and to Dr. Russell Peckham for ... popular and highly effective treatment for skin cancer. The selective fellowship in Mohs Micrographic ...
(Date:6/25/2016)... TX (PRWEB) , ... June 25, 2016 , ... ... the United States, named Dr. Sesan Ogunleye, as the Medical Director of its new ... the facility Medical Director of our new Mesquite location,” said Dr. James M. Muzzarelli, ...
(Date:6/25/2016)... ... June 25, 2016 , ... On Friday, June 10, Van Mitchell, ... at Work award to iHire in recognition of their exemplary accomplishments in worksite health ... annual Maryland Workplace Health & Wellness Symposium at the BWI Marriott in Linthicum Heights. ...
(Date:6/24/2016)... ... June 24, 2016 , ... Those who have experienced traumatic events may ... to unhealthy avenues, such as drug or alcohol abuse, as a coping mechanism. To ... for healthy coping following a traumatic event. , Trauma sufferers tend to feel a ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... DUBLIN , June 23, 2016 ... the "Pharmaceutical Excipients Market by Type (Organic Chemical ... Preservative), Formulation (Oral, Topical, Coating, Parenteral) - Global Forecast ... The global pharmaceutical excipients ... 2021 at a CAGR of 6.1% in the forecast ...
(Date:6/23/2016)... June 23, 2016 Research and Markets ... Medical Market Analysis 2016 - Forecast to 2022" report ... The report contains up to date financial data derived from ... of major trends with potential impact on the market during ... market segmentation which comprises of sub markets, regional and country ...
(Date:6/23/2016)... 2016 Bracket , a leading clinical trial ... clinical outcomes platform, Bracket eCOA (SM) 6.0, at the ... – 30, 2016 in Philadelphia , Pennsylvania.  ... Assessment product of its kind to fully integrate with RTSM, ... eCOA 6.0 is a flexible platform for electronic clinical outcomes ...
Breaking Medicine Technology: