Navigation Links
Building a biochemistry lab on a chip
Date:2/12/2013

Miniaturized laboratory-on-chip systems promise rapid, sensitive, and multiplexed detection of biological samples for medical diagnostics, drug discovery, and high-throughput screening. Using micro-fabrication techniques and incorporating a unique design of transistor-based heating, researchers at the University of Illinois at Urbana-Champaign are further advancing the use of silicon transistor and electronics into chemistry and biology for point-of-care diagnostics.

Lab-on-a-chip technologies are attractive as they require fewer reagents, have lower detection limits, allow for parallel analyses, and can have a smaller footprint.

"Integration of various laboratory functions onto microchips has been intensely studied for many years," explained Rashid Bashir, an Abel Bliss Professor of electrical and computer engineering and of bioengineering at Illinois. "Further advances of these technologies require the ability to integrate additional elements, such as the miniaturized heating element, and the ability to integrate heating elements in a massively parallel format compatible with silicon technology.

"In this work, we demonstrated that we can heat nanoliter volume droplets, individually and in an array, using VLSI silicon based devices, up to temperatures that make it interesting to do various biochemical reactions within these droplets."

"Our method positions droplets on an array of individual silicon microwave heaters on chip to precisely control the temperature of droplets-in-air, allowing us to perform biochemical reactions, including DNA melting and detection of single base mismatches," said Eric Salm, first author of the paper, "Ultralocalized thermal reactions in subnanoliter droplets-in-air," published in the Proceedings of the National Academy of Sciences (PNAS) on February 12.

According to Salm, approaches to perform localized heating of these individual subnanoliter droplets can allow for new applications that require parallel, time-, and space multiplex reactions on a single integrated circuit. Within miniaturized laboratory-on-chips, static and dynamic droplets of fluids in different immiscible media have been used as individual vessels to perform biochemical reactions and confine the products.

"This technology makes it possible to do cell lysing and nucleic acid amplification reactions within these individual droplets the droplets are the reaction vessels or cuvettes that can be individually heated," Salm added.

"We also demonstrate that ssDNA probe molecules can be placed on heaters in solution, dried, and then rehydrated by ssDNA target molecules in droplets for hybridization and detection," said Bashir, who is director of the Micro and Nanotechnology Laboratory at Illinois. "This platform enables many applications in droplets including hybridization of low copy number DNA molecules, lysing of single cells, interrogation of ligandreceptor interactions, and rapid temperature cycling for amplification of DNA molecules.

"Notably," Bashir added, "our miniaturized heater could also function as dual heater/sensor elements, as these silicon-on-insulator nanowire or nanoribbon structures have been used to detect DNA, proteins, pH, and pyrophosphates.

By using microfabrication techniques and incorporating the unique design of transistor-based heating with individual reaction volumes, 'laboratory-on-a-chip' technologies can be scaled down to 'laboratory-on-a-transistor' technologies as sensor/heater hybrids that could be used for point-of-care diagnostics."


'/>"/>

Contact: Rashid Bashir
rbashir@illinois.edu
217-333-3097
University of Illinois College of Engineering
Source:Eurekalert

Related medicine news :

1. American Association of Radon Scientists and Technologists (AARST) Releases Nation's First Standard for Mitigating Radon (Radioactivity) in Multifamily Buildings
2. Visual Impact Muscle Building Review Revealed Insight View Of Muscle Enlargement By Rusty Moore In His Program Visual Impact Muscle Building
3. Burn the Fat, Feed the Muscle: Review Examining Tom Venuto’s Training Program Released by QuickMuscleBuildingTips.com
4. Netmark Divulges Their Link Building Strategies
5. Hyper Gain: Review Examining ASR's Creatine Supplement Released By QuickMuscleBuildingTips.com
6. Visual Impact Muscle Building: Review Examining Rusty Moore's Training Program Released By DietsAndFitnessGuides.com
7. Rebuilding blood vessels through gene therapy
8. Steering stem cells to become 2 different building blocks for new blood vessels
9. Dental Marketing Formula.com has been Featured in The LA Daily News for their Specialized Practice Building Solutions in the Dental Industry
10. UC research explores relationship-building program for male same-sex couples
11. Rebuilding a whole heart for children born with only half of 1
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/9/2016)... ... 09, 2016 , ... Shark Finds and Kevin Harrington, and the Product Managers of ... GRIP-DRY is a newly patented product that has solved some of the basic problems golfers ... early morning dew or right after a rain shower, might understand the struggle of placing ...
(Date:2/8/2016)... ... February 08, 2016 , ... The Federal Laboratory Consortium for ... The site houses a wealth of federal resources that businesses can leverage to ... technology transfer (T2). As a network of over 300 federal laboratories, the FLC’s ...
(Date:2/8/2016)... , ... February 08, 2016 , ... ... in Houma, LA, celebrates the beginning of a new charity campaign. As part ... Court Appointed Special Advocates (CASA). In the belief that children deserve a voice, ...
(Date:2/8/2016)... Alexandria, Minn. (PRWEB) , ... February 08, 2016 ... ... introduces the BantamPro L top-load case packer for pouches, bags, and flow wrapped ... designed to help co-packers and specialty product manufacturers step up to semi-automatic or ...
(Date:2/8/2016)... ... February 08, 2016 , ... Steve Helwig & Associates Insurance & Financial, serving ... program, has teamed up with Citizens Opposed to Domestic Abuse in support of its ... support all those victimized by the fear of violence in their own homes, donations ...
Breaking Medicine News(10 mins):
(Date:2/9/2016)... , February 9, 2016 ... a collaboration with 10x Genomics to develop and ... biology and bioinformatics. --> QGEN ; ... with 10x Genomics to develop and promote comprehensive ... bioinformatics. --> QIAGEN N.V. (NASDAQ: ...
(Date:2/9/2016)... 9, 2016 Amarantus BioScience Holdings, ... developing products for Regenerative Medicine, Neurology and Orphan Diseases, ... company at Source Capital Group,s 2016 Disruptive Growth & ... in New York City . The ... 2016 at 12:30 pm by Gerald E. Commissiong ...
(Date:2/9/2016)... 2016 The leader in accelerated orthodontics, OrthoAccel ... is the recipient of the 2015 Townie Choice Award ... II medical device that speeds up orthodontic tooth movement ... often associated with treatment, AcceleDent was selected by orthodontists ... Orthotown survey of the most reliable and ...
Breaking Medicine Technology: