Navigation Links
Brain tumor cells penetrated by tiny, degradable particles carrying genetic instructions
Date:4/29/2014

ng their effectiveness. "Nanoparticles that remain stable for such a long time allow us to make up formulations well in advance and in large batches," says Stephany Tzeng, Ph.D., a member of Green's team. "This makes them easy to use consistently in experiments and surgeries; we add water to the particles, and they're good to go."

In a related study, published online on March 27 in the same journal, Green's group also showed that a different particle formulation could effectively carry and deliver so-called siRNAs to brain cancer cells. siRNAs are very small molecules that carry genetic information to cells, but unlike DNA that can turn genes on, siRNA interferes with the production of particular proteins and can turn cancer genes off.

Green explains that siRNAs must be encapsulated in particles that are different from those used to carry DNA because siRNAs are about 250 times smaller than the DNA molecules usually used for gene therapy. "siRNAs are also much stiffer than DNA, and they don't have to enter the cell nucleus because they do their work outside it, in the cytoplasm," he says.

An initial library of 15 biodegradable particle formulations was tested for their ability to carry siRNAs into human glioblastoma cells that were genetically engineered to make green fluorescent protein (GFP). The siRNAs added to the particles contained the GFP code, so successfully targeted cells would stop glowing green.

By tweaking the chemical properties of the particles, the team was able to find a composition that decreased GFP's glow in the human brain cancer cells by 91 percent. To test the ability of the particles to deliver death-inducing siRNAs, the team loaded the particles with a mixture of siRNA codes designed to prevent important proteins from being made. They then added these particles to brain cancer cells and to non-cancerous brain cells growing in the laboratory.

As in their mouse study, the siRNA was more effective i
'/>"/>

Contact: Catherine Kolf
ckolf@jhmi.edu
443-287-2251
Johns Hopkins Medicine
Source:Eurekalert  

Page: 1 2 3 4

Related medicine news :

1. Stroke risk reduced if brain blood vessel disorder is left alone
2. Diabetes duration and severity associated with brain atrophy
3. Precise brain mapping can improve response to deep brain stimulation in depression
4. Stanford scientists create circuit board modeled on the human brain
5. Study finds almost half of homeless men had traumatic brain injury in their life
6. Study finds almost half of homeless men had traumatic brain injury in their
7. Inaugural Brain Health Summit focuses on brain resilience and regeneration
8. Breast cancer replicates brain development process
9. Newly approved brain stimulator offers hope for individuals with uncontrolled epilepsy
10. Brain size matters when it comes to animal self-control
11. Sleep disorder linked to brain disease
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Brain tumor cells penetrated by tiny, degradable particles carrying genetic instructions
(Date:10/19/2014)... Hastings and Hastings, a Phoenix personal injury ... the greater Phoenix area and across Arizona reports record ... catastrophic automobile accidents. Statistics have indicated a steady increase ... As such, Hastings and Hastings has seen a steady ... who have been injured through no fault of their ...
(Date:10/19/2014)... 19, 2014 Dr. Babatunde Osotimehin, Executive ... news of a ceasefire agreement that is expected to ... girls who were kidnapped from the north-eastern Nigerian town ... captivity long enough, and it is high time they ... Dr. Osotimehin. , "We owe it to these girls ...
(Date:10/19/2014)... 2014 Recently, Top10BestSEOHosting.com has unveiled ... JustHost, GoDaddy and HostMonster are the best VPS ... Private Server) is the method of partitioning a ... which has the appearance and capabilities of running ... the site pays close attention to VPS products ...
(Date:10/19/2014)... Visual Impact Frequency Training - a ... to the public generating a frenzied buzz of ... community. The commotion surrounding the program's launch has ... an investigative review. , "Many gym-goers are familiar ... known as a training 'plateau' where they are ...
(Date:10/19/2014)... that causes inflammation in the spinal joints and ... ancient Egyptian royal families. Now a new study ... journal of the American College of Rheumatology (ACR), ... condition called diffuse idiopathic skeletal hyperostosis (DISH) in ... 20th Dynasties. , Ankylosing spondylitis is a ...
Breaking Medicine News(10 mins):Health News:Hastings And Hastings Reports Record Demand In 2014 For Legal Representation With Regard To Catastrophic Automobile Accidents 2Health News:Hastings And Hastings Reports Record Demand In 2014 For Legal Representation With Regard To Catastrophic Automobile Accidents 3Health News:The Best VPS Hosting Suppliers In The Market Unveiled By Top10BestSEOHosting.com 2Health News:Visual Impact Frequency Training Review: Rusty Moore's Latest Fitness Guide Released 2Health News:Mummy remains refute antiquity of ankylosing spondylitis 2
... offenders' lack of trust in medical professionals means - ... mental distress.// ,Men who have been incarcerated ... and under-utilise mental health services compared to the general ... with a health professional may predict the likelihood of ...
... School of Medicine have demonstrated the potential of a ... high cholesterol// levels. ,The findings are in ... Medicine (NEJM). In this study, patients with homozygous familial ... had a remarkable 51% reduction in low-density lipoprotein (LDL) ...
... tend to succumb more to respiratory infections in winter ... weather, combined with fog, like the capital has been ... viruses to spread that cause influenza type infections, say ... to the ill effects of low temperature. The influenza ...
... deficient in nutrients or calories,// and may not do much ... researchers from University of Ontario said. , ,The study ... hampers, sourced from a well established food bank located in ... the hampers, meant to last three days, contained calories sufficient ...
... studies prove conclusive.// , Scientists claim to have found ... of DNA found at the ends of the chromosome. ,Nilesh ... medical journal, The Lancet, detailed their experiments on 1500 men, in ... the length of their telomere (sampled from white blood cells) found ...
... use of viruses like that of the common cold ... ,Leonard Seymour, a professor of gene therapy at Oxford University, ... ,While the technique, called virotherapy, is not new, it ... which have spread, or in essence cancer cells, which have ...
Cached Medicine News:Health News:Offenders Unlikely to Seek Help 2Health News:New Therapy to Treat Patients With Severely Elevated Cholesterol Levels 2Health News:New Therapy to Treat Patients With Severely Elevated Cholesterol Levels 3Health News:Severe Cold is Harbinger of Infections 2Health News:Humble Cold to Combat Cancer 2
(Date:10/17/2014)... , October 17, 2014 /PRNewswire/ ... and Markets ( http://www.researchandmarkets.com/research/rxznjk/micro_market ) has announced ... Monitor : North American Automated External ... offering.  ,     (Logo: http://photos.prnewswire.com/prnh/20130307/600769) ,The report ... 2008-2018 analyzes the market of devices ...
(Date:10/17/2014)... The following is being released by ... The Green Park Collaborative (GPC) will bring together ... medical device and pharmaceutical companies, as well as ... 2015 at its first ever workshop on the ... Participants will discuss study designs to evaluate weight ...
(Date:10/17/2014)... --  InnFocus, Inc ., reported on results of 59 glaucoma ... for up to 3 years with the InnFocus MicroShunt® at ... Chicago . The Summit was ... the American Academy of Ophthalmology . The Company also ... in 6 countries by early 2015. Logo - ...
Breaking Medicine Technology:Micro Market Monitor : North American Automated External Defibrillators (AED) Market 2Green Park Collaborative to Discuss Comparative Effectiveness and Value of Weight Loss Treatments 2InnFocus Featured at the Ophthalmology Innovation Summit In Chicago 2
... Healthcare Products, Inc., (NASDAQ: AHPI ) reported ... hospitals and government entities still suffering from recession-driven budget cuts. ... per basic and diluted share, for the 2011 fiscal year compared ... per share, for the previous year. For the quarter, ...
... Sept. 2, 2011 Sigma-Aldrich Corporation (NASDAQ: ... conferences in September: KeyBanc Capital Markets ... 13, at the InterContinental, Boston, MA, presenting at 9:00 ... Wednesday, September 14, at the Grand Hyatt, New York, ...
Cached Medicine Technology:Allied Healthcare Reports Profitable Quarter and Year Despite Lingering Effects of Recession 2Allied Healthcare Reports Profitable Quarter and Year Despite Lingering Effects of Recession 3Allied Healthcare Reports Profitable Quarter and Year Despite Lingering Effects of Recession 4Sigma-Aldrich Corporation to Present at Upcoming Investor Conferences 2
... is a 60 watt diode continuous wave laser ... Diode Power Bar technology and 940 nm wavelength ... treatment for varicose veins and a variety of ... unique safety features including the LPS, Light guide ...
... Innovations' Instrument Manager (IM) is an instrument ... system that enables laboratories to work more ... as a data management solution that stands ... information system, filling those important gaps in ...
70 discs to investigate acquired color deficiency. Quantifies neutral zone characteristics of dyschromatopsia and evaluates the relative luminance....
... of the Farnsworth 100 Hue Test comes boxed ... and reduces the potential for test disruption ... Farnsworth 100 Hue test from Richmond Products includes ... tray, a durable laminated score template for copying, ...
Medicine Products: