Navigation Links
Boston University researchers validate important roles of iPSCs in regenerative medicine
Date:5/2/2011

(Boston) Researchers from Boston University's Center for Regenerative Medicine (CReM) have demonstrated that induced pluripotent stem cells (iPSCs) can differentiate into definitive endoderm cells, in vitro, with similar functional potential when compared to embryonic stem cells (ESCs), despite minor molecular differences between the two cell types. These findings are particularly important given growing controversy in the scientific literature about whether subtle differences between iPSCs and ESCs should dampen enthusiasm for iPSCs to serve as an alternative source of differentiated precursor cells for various tissues, such as the liver, lung or blood. The new work provides compelling evidence that iPSCs have potential in regenerative medicine as an investigational tool for the development of treatments against diseases that affect endodermal-derived organs, such as cirrhosis, diabetes, cystic fibrosis and emphysema.

Darrell Kotton, MD, an associate professor of medicine and pathology at Boston University School of Medicine (BUSM), served as principal investigator and senior author for this study, which is published online in the Journal of Clinical Investigation (JCI). Constantina Christodoulou, BS, from BUSM's program in genetics and genomics, was the lead author of the study.

iPSCs, discovered in 2006, are derived by reprogramming adult cells into a primitive stem cell state. They are similar to ESCs in terms of their ability to differentiate into different types of cells in vivo, including endoderm cells that give rise to liver and lung tissue. iPSCs do not require embryos and they are genetically identical to the patient's cells, suggesting their future potential to be transplanted back into the same patient without risk of rejection. Additionally, iPSCs could reduce the reliance on ESCs, which remain highly controversial and have limited availability due to federal regulation.

Recently, however, there has been debate regarding whether the molecular differences found in iPSCs make them as functional for research as ESCs when used in regenerative medicine research.

Kotton and his colleagues set out to understand the limits and potential of iPSCs and whether they should be utilized in research as a basis for the development of potential therapies. They focused their research on the capacity of iPSCs to undergo differentiation in vitro into endodermal tissue.

Working together with the laboratory of Gustavo Mostoslavsky, MD, PhD, assistant professor of medicine at BUSM, the teams of CReM investigators generated their own iPSC lines by reprogramming skin fibroblasts using a special stem cell cassette vector (STEMCC). They interrogated the global gene expression profiles of each cell line during endodermal differentiation and also compared the resulting cells to authentic endoderm from early developing mouse embryos.

"We found that although there are subtle molecular differences between iPSCs and ESCs, their functional potential to differentiate was virtually indistinguishable in vitro," said Kotton, who is a co-director of CReM. "It is important to understand that iPSCs offer many possibilities in regenerative medicine and developmental biology research and may hold the key to future medical treatments for many human diseases."

The next step, said Kotton, is to further differentiate iPSCs into more specific cell types using both mouse and human stem cell lines. CReM currently has 100 stem cell lines from donors with lung-specific diseases that will be used in the research to develop potential treatments against diseases that affect the lungs.


'/>"/>

Contact: Jenny Eriksen
jenny.eriksen@bmc.org
617-638-6841
Boston University Medical Center
Source:Eurekalert

Related medicine news :

1. Boston University researchers find most substance-dependent individuals report poor oral health
2. Boston University School of Medicine professor receives award for quality of care research
3. Boston researchers create SMArt platform architecture, launch $5,000 health app competition
4. Boston Medical Center critical care chief receives grant for lung injury study
5. Boston University School of Medicine professor co-authors first book on deaf ethnicity
6. Boston University School of Medicine awarded grants to improve early detection of lung cancer
7. Boston University researcher lauded for leading-edge lung cancer research efforts
8. Boston, Oct. 21-24: Largest international pediatric oncology conference
9. Boston Medical Center receives 2 grants for integrative medicine research
10. Boston Medical Center research study validates the Boston Bowel Preparation Scale
11. eMindful Provides a New Twist on Chocolate for Members of Boston's Beacon Hill Village
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... ... June 27, 2016 , ... ... professionals, announced today its affiliation with Tennessee Counseling Association. This new ... network of the Tennessee Counseling Association, adding exclusive benefits and promotional offers. , ...
(Date:6/26/2016)... ... June 26, 2016 , ... On June 10-11, 2016, A Forever Recovery, ... Festival and World’s Longest Breakfast Table in Battle Creek, MI, where the rehabilitation facility ... home to some of the world’s leading providers of cereal and other breakfast foods. ...
(Date:6/25/2016)... ... 2016 , ... Experts from the American Institutes for Research ... June 26-28, 2016, at the Hynes Convention Center in Boston. , AIR experts ... planning, healthcare costs and patient and family engagement. , AIR researchers will be ...
(Date:6/25/2016)... ... June 25, 2016 , ... As a lifelong Southern Californian, ... and his M.D from the David Geffen School of Medicine at UCLA. He trained ... Angeles to complete his fellowship in hematology/oncology at the UCLA-Olive View-Cedars Sinai program where ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... Those who ... with these feelings, many turn to unhealthy avenues, such as drug or alcohol abuse, ... Marne, Michigan, has released tools for healthy coping following a traumatic event. , Trauma ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... HILLS, Calif. , June 23, 2016 Any ... the many challenges of the current process. Many of them ... because of the technical difficulties and high laboratory costs involved. ... have to offer it at such a high cost that ... afford it. Dr. Parsa Zadeh , founder ...
(Date:6/23/2016)... INDIANAPOLIS , June 23, 2016 Roche ... received 510(k) clearance for its Elecsys BRAHMS PCT (procalcitonin) ... severe sepsis or septic shock. With this clearance, Roche ... provide a fully integrated solution for sepsis risk assessment ... associated with bacterial infection and PCT levels in blood ...
(Date:6/23/2016)... 23, 2016 Research and Markets has ... 52" report to their offering. ... creates a favourable commercial environment for MedImmune to enter. The ... that will serve to drive considerable growth for effective anti-influenza ... to cap sales considerably, but development is still in its ...
Breaking Medicine Technology: