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Biotechnology Builds a New Heart
Date:1/13/2008

Stem cells injected into 'skeleton' of animal organ was beating in just 8 days

SUNDAY, Jan. 13 (HealthDay News) -- An organ-building biotechnology that could create transplantable hearts using stem cells from the recipients themselves has passed important laboratory tests, researchers report.

The technique, called whole organ decellularization, has been used to create functioning heart tissue, according to a report in the Jan. 13 issue of Nature Medicine by a team at the University of Minnesota Center for Cardiovascular Repair.

What the scientists did first was to use detergents and other chemicals to wash out all the old heart cells from rat and pig hearts, explained lead investigator Doris Taylor, who is director of the center. What was left was a scaffold of tubes that once were the organ's blood vessels. Stem cells were then injected into that scaffold, where they were supplied with nutrients that allowed them to grow to create a new organ. Within eight days, the hearts were pumping.

The basic technology is not new, Taylor said. It is being used to create many of the heart valves that are implanted in current operations, she said. However, heart valves are relatively small.

"We have done it on the scale of a pig heart, which is the size and scale of a human heart," she said. "A human heart would be the next step, but we want to perfect the technique first."

The Minnesota researchers have applied for a patent on the combination of agents they use to decellularize an organ the size of a human heart and provide nutrients to the implanted stem cells.

"Multiple things" must be done before a human trial can be attempted, Taylor said. "We are moving to larger organs, making sure we can get enough cells in to repopulate the entire heart, and also, if it is transplantable, to keep it alive for a long time," she said.

The biotechnology might be able to bypass a major impedim
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