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Bioreactors might solve blood-platelet supply problems
Date:12/23/2008

m one arm, passing it through a machine that isolates the platelets, and then returning it to the other arm. The method yields four to six platelet units per donor.

For this study, Lasky and his colleagues isolated hematopoietic stem cells, which produce blood cells, from blood taken from umbilical cords following normal, full-term deliveries. The stem cells were grown to greater numbers, then added to the bioreactors chambers with several layers for gas and growth-media control. Control cells were grown in culture flasks. Other attempts to grow platelets have usually used culture flasks or similar two-dimensional systems.

After a few days of growth, a solution of growth factors was added to both groups to stimulate the cells to form large, bone-marrow cells called megakaryocytes, which shed bits of themselves as platelets.

The three-dimensional bioreactor produced up to 1.2 million platelets per day, with production continuing for more than 32 days, while the two-dimensional system generated a maximum of about 350,000 platelets per day over a ten-day period.

Lasky and his colleagues are now modifying the process to increase the yield of platelets.


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Contact: Darrell E. Ward
darrell.ward@osumc.edu
614-293-3737
Ohio State University Medical Center
Source:Eurekalert

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