Navigation Links
Bioreactors might solve blood-platelet supply problems
Date:12/23/2008

COLUMBUS, Ohio It might be possible to grow human blood platelets in the laboratory for transfusion, according to a new study at The Ohio State University Medical Center.

The findings, published in the January 1, 2009 issue of the journal Experimental Hematology, might one day enable blood banks to grow platelets continuously and in quantities that can ease the chronically tight supply of these critical blood components.

About 13 million platelet concentrates are collected annually in the United States at a cost of about $1 billion. They are needed by people who lack platelets or whose platelets function improperly, such as certain cancer chemotherapy patients, bone marrow transplant patients, trauma patients given massive blood transfusions and people with aplastic anemia.

The concentrates from volunteer donors are expensive to make, require 10 or more tests for pathogens and have a shelf life of only five days. As a result, 20 to 40 percent of platelet concentrates are discarded. Red blood cells, by contrast, last 56 days.

The short shelf life means platelets cannot easily be shipped from an area of surplus to one of scarcity, and hospitals occasionally experience shortages that require surgeries to be postponed.

Attempts by others to grow platelets have produced only small numbers for a short time, says principal investigator, Larry C. Lasky, associate professor of pathology at Ohio State and a specialist in transfusion medicine and blood banking.

"We were pleasantly surprised to achieve continuous production for a month," Lasky says. "It is easy to imagine a series of these chambers producing platelets. It would be ideal for clinical use and possibly solve the short shelf-life problem. Using good manufacturing practices would prevent bacterial contamination."

Currently, platelets are collected either from donated blood or by apheresis. Apheresis is an expensive and time-consuming process that involves taking blood from one arm, passing it through a machine that isolates the platelets, and then returning it to the other arm. The method yields four to six platelet units per donor.

For this study, Lasky and his colleagues isolated hematopoietic stem cells, which produce blood cells, from blood taken from umbilical cords following normal, full-term deliveries. The stem cells were grown to greater numbers, then added to the bioreactors chambers with several layers for gas and growth-media control. Control cells were grown in culture flasks. Other attempts to grow platelets have usually used culture flasks or similar two-dimensional systems.

After a few days of growth, a solution of growth factors was added to both groups to stimulate the cells to form large, bone-marrow cells called megakaryocytes, which shed bits of themselves as platelets.

The three-dimensional bioreactor produced up to 1.2 million platelets per day, with production continuing for more than 32 days, while the two-dimensional system generated a maximum of about 350,000 platelets per day over a ten-day period.

Lasky and his colleagues are now modifying the process to increase the yield of platelets.


'/>"/>

Contact: Darrell E. Ward
darrell.ward@osumc.edu
614-293-3737
Ohio State University Medical Center
Source:Eurekalert

Related medicine news :

1. Small Doses of Carbon Monoxide Might Help Stroke Victims
2. New Genetic Analysis Might Boost Breast Cancer Care
3. Hypertension Might Hinder Thinking
4. If Your Child Says School Makes Him Sick - He Might Be Right
5. C-Section Might Boost Babys Asthma Risk
6. Fat-Sensing Hormones Might Fight Obesity
7. Gene Screen Might Predict Prostate Cancer
8. Early Life Peanut Consumption Might Prevent Allergy
9. Heartbeat Might One Day Power Pacemakers
10. New Compound Might Be Improvement Over Allergy Shots
11. Statin Might Help More People Fight Heart Disease Than Thought
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/25/2017)... ... September 25, 2017 , ... “The Ministry of Reconciliation: ... look at the religious stand in the sensitive matter of sexual discrimination, and ... Ministry of Reconciliation: A Compassionate and Realistic Approach to Evangelizing the LGBTQ Community” ...
(Date:9/25/2017)... Ignite Conference (PRWEB) , ... September 25, 2017 , ... ... have teamed up to deliver Netmail’s Hadron Data Auditing and eDiscovery ... Cloud. Netmail will be demo-ing their Hadron solution in a HIPAA/HITRUST Azure Cloud at ...
(Date:9/25/2017)... ... September 25, 2017 , ... ... Workshop, **Presented by FDAnews**, Nov. 1-3, 2017 – Bethesda, MD, http://www.fdanews.com/fdainspectionssummit ... flying colors. And then, on the tenth inspection it failed. , What went ...
(Date:9/24/2017)... ... September 25, 2017 , ... Bhowanie Benimadhu’s new book “ Our ... questions of life and seeks answers in both Eastern and Western religious traditions and ... God is in the mind of humankind for the past thousands of years and ...
(Date:9/24/2017)... , ... September 23, 2017 , ... Throughout the United ... harm. , Every day, physicians, nurses and other providers work to give the ... innovative industry processes. , But U.S. Senators today are threatening to tie our ...
Breaking Medicine News(10 mins):
(Date:9/18/2017)... Sept. 18, 2017 EpiVax, Inc. ... bioinformatics and immune engineering, today announced a ... A (H7N9) vaccine. ... seasonal influenza and presents a challenge for ... exposure to be effective. Using state-of-the-art bioinformatics and ...
(Date:9/12/2017)...  ValGenesis Inc., the global leader in Enterprise ... announce the appointment of Dr. Ajaz Hussain ... Directors and Chairman of Advisory Board beginning September ... to manage their entire validation lifecycle process electronically ... process. Furthermore, ValGenesis VLMS enables rigorous compliance, helps ...
(Date:9/7/2017)... Sept. 7, 2017  Eli Lilly and Company ... to streamline operations to more efficiently focus resources ... cost structure. Global workforce reductions, including those from ... to impact approximately 3,500 positions. ... annualized savings of approximately $500 million that will ...
Breaking Medicine Technology: