Navigation Links
Biologists develop efficient genetic modification of human embryonic stem cells
Date:1/7/2010

Biologists have developed an efficient way to genetically modify human embryonic stem cells. Their approach, which uses bacterial artificial chromosomes to swap in defective copies of genes, will make possible the rapid development of stem cell lines that can both serve as models for human genetic diseases and as testbeds on which to screen potential treatments, they say.

"This will help to open up the whole human embryonic stem cell field. Otherwise, there's really few efficient ways you can study genetics with them," said Yang Xu, professor of biology at the University of California, San Diego who directed the research. Xu and co-authors Hoseok Song and Sun-Ku Chung, both postdoctoral fellows in Xu's research group, describe their technique in the January 8 issue of the journal Cell Stem Cell.

Most attempts to alter the genetic makeup of the cells have proved too inefficient, Xu said. His group used bacterial artificial chromosomes, or BACs, to improve the yield.

BACs are synthesized circles of human DNA, which bacteria will replicate just like their own native chromosomes. Commercially available BACs can be modified within bacterial cells to insert altered copies of specific genes. Once the modified BACs are introduced into human cells, they will sometimes pair up with a matching segment of a human chromosome and swap segments of DNA, a process called homologous recombination.

The advantage of using BACS to alter the genetic code in human cells comes from the long flanking sequences on either side of the modified gene, which increases the chance that the BAC with line up with native DNA in position for a swap. Other genetic approaches have been limited by shorter segments of DNA.

Using BACs, the team was able to substitute modified genes in 20 percent of treated cells. Standard methods of genetic modification typically achieve modification in fewer than one percent of cells, Xu said.

His group s
'/>"/>

Contact: Yang Xu
scinews@ucsd.edu
University of California - San Diego
Source:Eurekalert

Page: 1 2 3

Related medicine news :

1. Biologists Question Animal Brain Size Theory
2. Where tumor cells boldly go: Weill Cornell cancer biologists shed light on the metastatic niche
3. Cell biologists identify new tumor suppressor for lung cancer
4. Microbiologists receive top Canadian recognition
5. Boston College biologists build a better mouse model for cancer research
6. Biologists Discover How Dengue Virus Matures
7. FDA clears TransOral robotic surgery developed at Penn
8. Michael J. Fox Foundation Awards $1 Million for Critical Step Toward Development of Dyskinesia Treatments
9. Nu-Tek Products Develops Cultured Soymilk Powder
10. Milton & DeKruif Launches New Client Development Campaign
11. Safety and Quality Software Developer Quantros, Inc. Launches Healthcare IT Consulting Division
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/28/2015)... ... August 28, 2015 , ... The 6th Annual Survivor in the City, ... added yet another accolade to its cadre of widespread support—an official letter of support ... help of the medical community and the University of Illinois at Chicago’s Department of ...
(Date:8/28/2015)... ... August 28, 2015 , ... Saint Petersburg, FL —Pocket Protein™, ... fat, carbs, sugar, gluten, lactose or caffeine in a convenient 2 oz. pouch ... CPG Editor’s Choice Awards. , Five consumer packaged goods (CPG) products were selected ...
(Date:8/28/2015)... ... August 28, 2015 , ... Drugs and ... James Strawbridge, gives a new approach on solutions in his new book ":Unraveling ... employer and employees getting together for meaningful group meetings. "This would educate the ...
(Date:8/28/2015)... ... 28, 2015 , ... CARF International announced that West Ridge ... treatment and intensive family based services. , By pursuing and achieving accreditation, West ... of excellence. As a nonprofit charity for more than 50 years, West Ridge ...
(Date:8/28/2015)... Rochester, NY (PRWEB) , ... August 28, 2015 , ... ... injectable, Kybella™. It is the first and only nonsurgical product approved by the ... Dr. Vito Quatela and Dr. William Koenig are among ...
Breaking Medicine News(10 mins):Health News:Chicago Mayor Pens Official Support of Annual Survivor in the City Event Benefitting Brain Aneurysm Foundation 2Health News:Chicago Mayor Pens Official Support of Annual Survivor in the City Event Benefitting Brain Aneurysm Foundation 3Health News:Pocket Protein™ Named 2015 CPG Editor’s Choice Award Finalist by Informa’s SupplySide 2Health News:Pocket Protein™ Named 2015 CPG Editor’s Choice Award Finalist by Informa’s SupplySide 3Health News:West Ridge Academy Awarded CARF Three-Year Accrediation 2Health News:Non-surgical Treatment to Get Rid of The "Double Chin," Kybella™, Now Available at the Quatela Center for Plastic Surgery 2Health News:Non-surgical Treatment to Get Rid of The "Double Chin," Kybella™, Now Available at the Quatela Center for Plastic Surgery 3
... News) -- A growing number of Americans are getting advice ... rather than friends, a new survey indicates. About ... media such as Facebook and Twitter, a substantial increase from ... who sought information from friends fell from 63 percent in ...
... Moffitt Cancer Center in Tampa, Fla., have discovered that ... roles in tobacco-related non-small cell lung cancers. Tobacco carcinogens ... The study was published in a recent issue ... identified oconogene, a gene linked to cancer," said study ...
... BOSTON (2-8-12) Observing that certain cancer cells may ... that this capability promotes rapid tumor growth. Now computer ... Muhammad Zaman and collaborators at the University of Texas ... model of healthy and cancer cells that they,ve created ...
... In a study to be presented today at the ... , in Dallas, Texas, researchers will report findings that ... section appear to know little about the risks and ... trial of labor to attempt a vaginal delivery, and ...
... TAMPA, Fla. and ORLANDO, Fla. Moffitt Cancer ... have announced they will collaborate on the creation ... Florida). The partnership will conduct collaborative research to ... areas of cancer and metabolic diseases, including obesity, ...
... supports an association between atypical fractures of the femur a ... and the use of bisphosphonates, drugs proven to enhance bone ... risk for suffering an atypical femur fracture while taking bisphosphonates ... after six years of treatment research presented today at ...
Cached Medicine News:Health News:More Patients Seek Plastic Surgery Advice From Facebook, Twitter 2Health News:Researchers find important 'target' playing role in tobacco-related lung cancers 2Health News:Boston University research suggests new pathways for cancer progression 2Health News:Study finds pregnant women with prior cesarean choose the delivery method preferred by their doctor 2Health News:Moffitt, Sanford-Burnham and Florida Hospital create Personalized Medicine Partnership of Florida 2Health News:Moffitt, Sanford-Burnham and Florida Hospital create Personalized Medicine Partnership of Florida 3Health News:Halting bone-building osteoporosis drug use cuts risk for additional atypical femur fracture in half 2
(Date:8/28/2015)... SEOUL, South Korea , Aug. 28, 2015 /PRNewswire/ ... Chungbuk National University and Dr. Ra from Biostar ... , Confirmed 30% increase of lifespan through multiple ... cognitive function, physical vitality, and life expectancy found ... Researchers in Korea have determined the possibility that adult ...
(Date:8/27/2015)... , Aug. 27, 2015  The Academy of ... and Drug Administration,s (FDA,s) draft guidance and proposed ... and biosimilars, to bear a nonproprietary name with ... been seeking a decision from the FDA on ... health care stakeholders urging the agency to use ...
(Date:8/27/2015)... -- According to a new market research report "Medical ... Bariatric ), Material (Nylon, Padded, Canvas), Usage Type (Disposable, Reusable), End ... the Medical Lifting Sling Market is poised to reach USD 706.0 ... CAGR of 11.4 % from 2015 to 2020. ... T ables and 65 F ...
Breaking Medicine Technology:Korean Researchers Find the Possibility that Adult Mesenchymal Stem Cells (MSCs) May Extend Lifespan 2AMCP Is Disappointed in FDA's Draft Guidance and Proposed Rule Calling for Suffix on Nonproprietary Names of Biological Products 2Medical Lifting Sling Market Worth USD 706.0 Million by 2020 2Medical Lifting Sling Market Worth USD 706.0 Million by 2020 3Medical Lifting Sling Market Worth USD 706.0 Million by 2020 4
... 2011 Hurricane Irene came rushing up the East Coast as ... the United States, many states in the Northeast began to worry. ... to hurricanes. Coastal towns from New Jersey all the way up ... York City was one that took steps that had not been ...
... DIEGO, Aug. 30, 2011 ADVENTRX Pharmaceuticals, Inc. (NYSE ... on its planned meetings with the U.S. Food and ... Exelbine (vinorelbine injectable emulsion) Type ... is to discuss the complete response letter regarding the ...
Cached Medicine Technology:Hospitals Evacuated in Preparation for Hurricane Irene with Aid of Pulse Oximeter 2ADVENTRX Provides Update on All Three Pipeline Products 2ADVENTRX Provides Update on All Three Pipeline Products 3ADVENTRX Provides Update on All Three Pipeline Products 4
Denman brown nucleus cracker with 30 angled shafts, tip to angle length 14.5 mm. Standard cross action handle. 1.1 mm x 1.2 mm paddle style tips....
Ernest nucleus cracker extra delicate cross-action serrated paddle shaped jaws for cracking the nucleus through a 2.5 mm incision....
Epilation forceps straight ends size 3.5"....
Removal Forceps, very fine pointed tips, with textured jaws, ideal for grasping wet sutures....
Medicine Products: