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Bio-Rad's Droplet Digital PCR technology highlighted at the 2014 AACR Annual Meeting
Date:4/5/2014

levels of mutant and total BRAF DNA in the plasma of patients with stage IV melanoma change as the disease progresses. The study also demonstrated that ddPCR could be used to quantify those changes, showing promise that this assay could be used to predict disease progression.

Polsky's poster, titled "Quantitative assessment of circulating BRAF DNA in stage IV melanoma patients undergoing BRAF inhibitor treatment," will be shown on April 7, 1 5 PM in Hall A-E (Abstract Number 2847, Poster Section 37, Poster Board Number 28).

Comparatively Easy Quantification of Genetic Aberrations and Other Promising New Applications of ddPCR

A research group headed by Dr. Hanlee Ji, an assistant professor at the Stanford University School of Medicine, will present a new ddPCR method that allows researchers to investigate genetic aberrations without using traditional fluorogenic probes. His research group developed a new technique using a nonspecific DNA binding dye that is faster and more cost-effective than the use of TaqMan hydrolysis probes for applications such as copy number variation (CNV) and single nucleotide variation (SNV) assessment. This has broad applicability for the assessment of genetic aberrations associated with cancer.

Ji's poster, titled "Highly sensitive and specific digital quantification of cancer genetic aberrations," will be shown on Monday, April 7, 8 AM - PM in Hall A-E (Abstract 1507, Poster Section 21, Poster Board Number 6).

Bio-Rad application scientists will demonstrate additional novel applications of ddPCR including single-cell transcript profiling as a more sensitive, cost-effective, and rapid alternative to RNA-Seq; rapid, inexpensive and high-throughput cross-validation of NGS methylated tar
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Contact: Ken Li
kli@chempetitive.com
312-532-4675
Chempetitive Group
Source:Eurekalert

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