Navigation Links
Beckman Coulter Life Sciences Webinar Pinpoints Precise Quantification of Cell Viability
Date:8/26/2015

The Webinar, hosted by LabRoots, will focus on a protocol for the simultaneous detection of plasma membrane integrity or nuclear morphology, mitochondrial functions, and caspase activation, September 15, 2015

Cellular viability is customarily determined by measuring the cells’ capacity to exclude vital dyes (eg, 4',6-diamidino-2-phenylindole (DAPI)), or by evaluating nuclear morphology with chromatinophilic plasma membrane-permeant dyes, such as Hoechst 33342.

These methods are not foolproof, though. A fraction of cells that exclude DAPI, or even some that display normal nuclear morphology, have already lost mitochondrial functions and/or manifest massive activation of apoptotic caspases, and thus are irreversibly doomed to death.

Is there a clear-cut approach for pinpointing these conundrums?

As part of the Beckman Coulter Webinar Series, Beckman Coulter Life Sciences is sponsoring a new, free educational webinar, “Less False Negatives: Quantifying Cell Viability by Simultaneous Triple Staining.” Experts will discuss a protocol they developed for the simultaneous detection of plasma membrane integrity (based on DAPI) or nuclear morphology (based on Hoechst 33342), mitochondrial functions (based on the mitochondrial transmembrane potential probe DiOC6(3)), and caspase activation (based on YO-PRO®-3, which can enter cells exclusively upon the caspase-mediated activation of pannexin 1 channels). This method, which provides for the precise quantification of dead, dying, and healthy cells, can be performed on epifluorescence microscopy or flow cytometry platforms and is compatible with a robotized, high-throughput workflow.

The speakers are Oliver Kepp, PhD, research scientist, Kroemer Lab, Paris, and Allan Sauvat, research engineer, Institut Gustave Roussy, Villejuif, France, and the Kroemer Lab.

Dr. Kepp received his PhD in 2006 from the Humboldt University of Berlin and the Max Planck Institute for Infection Biology, Berlin. He is presently a research scientist in the laboratory of Guido Kroemer, MD, PhD, where Dr. Kepp investigates numerous aspects of immunogenic cell death, focusing on systems biology approaches.

Allan Sauvat earned his engineer’s degree in 2011 from the Institut Supérieur de BioSciences, Paris, and is now a research engineer on the BioCell platform in Villejuif, where he is focusing on HTS assay and software development for systems biology.

The complementary webinar, hosted by LabRoots, will be presented on September 15, 2015, at 7 am Pacific Time/10 am Eastern Time.

For full details and free registration, click here.

About Beckman Coulter:
Beckman Coulter develops and manufactures products that simplify, automate, and innovate complex biomedical testing. More than 275,000 Beckman Coulter systems operate in both diagnostics and life sciences laboratories on seven continents. For more than 75 years, the company’s products have been making a difference in peoples' lives by improving the productivity of medical professionals and scientists, supplying critical information for improving patient health, and delivering trusted solutions for research and discovery.

About LabRoots:
LabRoots is the leading professional networking website designed to connect all science verticals. Founded in March 2008, LabRoots’ vision was to connect the scientific world leveraging a myriad of unique features and tools, discovering meaningful collaborations across geographic boundaries and fields of expertise. LabRoots is the owner and producer of BioConference Live – which has grown into the world’s largest series of virtual events within the Life Sciences and Clinical Diagnostics community.

Read the full story at http://www.prweb.com/releases/2015/08/prweb12916601.htm.


'/>"/>
Source: PRWeb
Copyright©2015 Vocus, Inc.
All rights reserved

Related medicine news :

1. Beckman Coulter Webinar Highlights the Use of Analytical Ultracentrifugation to Characterize Macromolecules
2. Beckman Coulter Webinar Presents the Idea that Retroviruses Exploit the Exosomal Machinery to Generate Infectious Viral Particles
3. Beckman Coulter Webinar Highlights Large-scale Purification of RNA and RNA-based Nanoparticles
4. Beckman Coulter Webinar Discusses Quantitative Determination of Reaction Stoichiometry, Interaction Energies, and Solute Masses for Sedimentation Analysis
5. Physicians Choice Laboratory Services (PCLS) is Now Offering Beckman Coulter Diagnostics’ Prostate Health Index (phi).
6. Beckman Coulter Genomics Presents Using Next-Gen Sequencing to Study the Evolution of Neural Circuitry
7. Beckman Coulter Webinar Series Presents Centrifugal Elutriation- Utility in the Flow Cytometry Laboratory
8. Beckman Coulter Webinar Series Presents Insight into New Demands in Counting Particles in Liquids
9. Beckman Coulter Webinar Series Presents Advances in Sedimentation Analysis
10. Beckman Coulter Webinar Series Presents Diversity of Extracellular Vesicles and Their Cargo in Cell-to-cell Communication
11. Vixiar Medical™ Presents at Coulter Foundation Translational Research Investment Forum in Palo Alto
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/23/2017)... ... June 23, 2017 , ... MD Now Urgent Care ... MD Now’s 28th facility overall and marks the urgent care center's eighth location in ... North of The Falls shopping mall. The new clinic offers a wide array of ...
(Date:6/23/2017)... ... ... American Farmer, will feature Chr. Hansen, Inc (CHR Hansen) in an upcoming episode ... Farmer airs Tuesdays at 8:30aET on RFD-TV. Check your local listings for more info. ... 1874 after a groundbreaking discovery of how to industrialize enzymes. Rooted in science and ...
(Date:6/23/2017)... Chicago, IL (PRWEB) , ... June 23, 2017 ... ... insurance management assistance and financial planning services to communities in the greater Chicago ... Children’s Association to offer assistance to underprivileged youth in Chicago. , Founded in ...
(Date:6/23/2017)... ... June 23, 2017 , ... Moore Insurance, ... area with access to asset protection and financial planning services, is teaming up ... improving the lives of children with cancer and other chronic diseases. , The ...
(Date:6/22/2017)... ... June 22, 2017 , ... ... is pleased to welcome Whipple & Company as its newest Partner Firm. Headquartered ... of balancing their clients’ risk while tailoring optimized benefit packages that strengthen the ...
Breaking Medicine News(10 mins):
(Date:6/2/2017)... , June 2, 2017  NxStage Medical, Inc. (Nasdaq:  NXTM), a ... today announced new findings demonstrating positive biochemical outcomes related ... System One™. The data will be presented at the ... Madrid, Spain . The ... Home Dialysis Network in Europe ...
(Date:5/29/2017)... Israel, May 29, 2017  Cellect Biotechnology Ltd. (NASDAQ: ... technology which enables the functional selection of stem cells, ... for the first quarter ended March 31 st , ... our accomplishments in the first quarter of 2017," said ... quarter, we announced the treatment of the first blood ...
(Date:5/22/2017)... VALLEY COTTAGE, N.Y. , May 22, 2017 ... natural antiviral treatment Gene-Eden-VIR/Novirin, announces the launch of ... in plain English, the results of a clinical ... after one year treatment with Gene-Eden-VIR/Novirin in individuals ... is important to note that there are no ...
Breaking Medicine Technology: