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Beating a Global Killer: HIV/AIDS
Date:4/1/2009

for the pathogenesis of resistant profiles of minority subspecies.

Methods

Flow cytometric analysis of these cells was conducted using DC monoclonal antibodies and Annexin-V. A Biacore assay system that measures changes in the surface mass concentration was used to determine interactions between IPF molecules and CD4+ cells. Changes were expressed in resonance units (RU), with one RU representing a change in concentration of 1 pg/mm. T-cells were purified from peripheral blood mononuclear (PBMC) cells using antibody coated magnetic beads.

Results

According to David Miller, V.P. Governmental Affairs and Clinical Development of Immunotech Laboratories, and founder of ACT UP HIV/AIDS activist group, "Laboratory analysis indicates that IPF is able to mediate maturation of dendrite cells in vitro, as determined by up-regulation of MHC class-ll, CD86 and CD83 molecules, regulate pro-inflammatory cytokines IL-12 and INFy, and enhanced T-cells' stimulatory capacity. Observable characteristics include modulation of complement activation, saturation of Fc receptors on macrophages, and suppression of various inflammatory mediators, including cytokines and chemokines. IPF demonstrated increased synthesis of Th-1 cells. IPF displayed spontaneous binding with gp41 when prepared for gel electrophoresis, and subsequent fusion inhibition of HIV with CD4+ cells and increased gp41 and gp120 antigenic activity. Virus-specific CD8 cells were stimulated. Flow cytometric analysis revealed apoptosis in CD4+ cells and stimulation of virus-specific CD8 cells."

Conclusions

According to Harry Zhabilov, Chief Science Officer of Immunotech Laboratories, "IPF appears to modulate helper Th-1 cells' expression of elaborate cytokines INFy, IL-2, which selectively promote cell-mediated immune response and subsequently stimulate cytotoxic lymphocytes. These ly
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SOURCE Immunotech Laboratories, Inc.
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