Navigation Links
BT-R3 mediates killing of the malaria vector Anopheles gambiae by Bacillus thuringiensis
Date:8/23/2013

Researchers at The University of Texas at Dallas (UTD), led by Dr. Lee Bulla, have demonstrated for the first time the selective cytotoxicity of Bacillus thuringiensis subsp. israelensis Cry4B toxin is mediated by BT-R3. The Cry toxins produced by Bacillus thuringiensis exert their insecticidal activity by binding with high-affinity to their cognate cadherin receptors located on the surface of epithelial cells that line the midgut of susceptible insects. In the case of Anopheles gambiae, binding of the Cry4B toxin by BT-R3, in turn, triggers an internal signaling event that turns on a cell death pathway. The novelty of the research done by the UTD scientists is that they were able to establish the direct involvement of the BT-R3 receptor, cloned from Anopheles gambiae, in mediating toxicity of the Cry4B toxin in living cells. The research reported in this article in the July 2013 issue of Experimental Biology and Medicine is a culmination of proteomics, genomics and bioinformatics strategies developed in the Bulla laboratory.

Validation of BT-R3 as a functional receptor for Cry4B exemplifies the power of proteomics, genomics and bioinformatics to identify target proteins such as the BT-R3 receptor. The process of target selection starts with data mining of archived protein sequences available in various genome and proteome databases, and results in the selection and annotation of candidate proteins based on their potential to mediate insecticidal action. It brings together genome- and proteome-based target identification and target-directed screening for validating the action of insecticidal proteins such as the Cry4B toxinengineered or otherwise. Targets selected for consideration can then be analyzed in silico by docking calculations, molecular dynamics simulations and other techniques to characterize appropriate target interactions with chemically or genetically altered Cry toxins.

Dr. Mohamed Ibrahim, senior author of the paper, said "this kind of strategy will facilitate protein design for creation of new customized Cry proteins and peptide mimics that might be more effective than the natural toxins themselves against Anopheles gambiae and other mosquitoes, and, hopefully, less able to bring about insect host resistance."

New approaches are needed in the area of malaria control because no effective vaccine currently exists and no available anti-malarial medications have been designed or discovered that impede parasite resistance with long-term use. A few drugs have been able to provide relief to patients but a preventive anti-malarial vaccine is yet to be developed. Certainly, genetic modification of wild mosquito vector populations to reduce vectorial capacity is fraught with unknown and hidden trials and tribulations.

Dr. Steve Goodman, Editor-in-Chief of Experimental Biology and Medicine said "Lee Bulla and his colleagues have combined genomics, proteomics and in silico analysis to establish the role of the cadherin receptor BT-R3 in the killing action of Cry4B mosquito toxin. Understanding of this Cry4B-BT-R3 complex will allow the design of custom proteins and peptides to help control the spread of malaria by mosquitoes".


'/>"/>

Contact: Dr. Lee A. Bulla, Jr.
bulla@utdallas.edu
Society for Experimental Biology and Medicine
Source:Eurekalert

Related medicine news :

1. Resistance is futile: Researchers identify gene that mediates cisplatin resistance in ovarian cancer
2. Coronary artery disease continues to be neglected in women, despite it killing at least as many women as men
3. Critical Acclaim Pouring in for Killing Ideas: You Can Kill an Idea, You Can’t Kill an Opportunity by Dr. Pam Henderson: Book Shows Companies How to Maximize Growth Through BIG Opportunities
4. Humane, or Just Hypocritical? Lawyers at Console & Hollawell Respond to PETA Killing Controversy
5. Find Out Why Online Job Applications are Killing Employment Chances with a Complimentary Webinar on May 14th from Career Confidential
6. Get New Patients, INC Releases New Infographic titled 'Are Negative Reviews Killing Your Medical Practice?"
7. Paired CT scans catch chemo-killing of liver tumors in real time
8. Theranostic imaging offers means of killing prostate cancer cells
9. Talk to Your Kids About the Colorado Killings
10. Wartime Killing May Raise Veterans Thoughts of Suicide
11. Killing in war linked with suicidal thoughts among Vietnam veterans, study finds
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2016)... ... , ... On Friday, June 10, Van Mitchell, Secretary of the Maryland Department ... in recognition of their exemplary accomplishments in worksite health promotion. , The Wellness at ... Wellness Symposium at the BWI Marriott in Linthicum Heights. iHire was one of 42 ...
(Date:6/24/2016)... , ... June 24, 2016 , ... Those who have ... these feelings, many turn to unhealthy avenues, such as drug or alcohol abuse, as ... Michigan, has released tools for healthy coping following a traumatic event. , Trauma sufferers ...
(Date:6/24/2016)... Texas (PRWEB) , ... June 24, 2016 , ... ... International Conference and Scientific Sessions in Dallas that it will receive two significant ... of the grants came as PHA marked its 25th anniversary by recognizing patients, ...
(Date:6/24/2016)... ... 24, 2016 , ... Comfort Keepers® of San Diego, CA is excited to ... Recovery® program to drive cancer patients to and from their cancer treatments. Comfort ... quality of life and ongoing independence. Getting to and from medical treatments is ...
(Date:6/24/2016)... (PRWEB) , ... June 24, 2016 , ... ... today at the Clinical Decision Making in Emergency Medicine conference in Ponte Vedra ... authored journal articles published in Emergency Medicine Practice and Pediatric Emergency ...
Breaking Medicine News(10 mins):
(Date:6/23/2016)... 23, 2016 Research and Markets ... Market by Type (Organic Chemical (Sugar, Petrochemical, Glycerin), Inorganic ... Coating, Parenteral) - Global Forecast to 2021" report ... The global pharmaceutical excipients market is projected to ... of 6.1% in the forecast period 2016 to 2021. ...
(Date:6/23/2016)... Capricor Therapeutics, Inc. ... company focused on the discovery, development and commercialization ... in its ongoing randomized HOPE-Duchenne clinical trial (Halt ... its 24-patient target. Capricor expects the trial to ... 2016, and to report top line data from ...
(Date:6/23/2016)... RIDGE, Ill. and INDIANAPOLIS ... of students receiving a Lilly Diabetes Tomorrow,s Leaders Scholarship ... The 2016 scholarship winners, announced today online at ... to let type 1 diabetes stand in the way ... Diabetes has supported the Foundation,s scholarship program since 2012, ...
Breaking Medicine Technology: