Navigation Links
Aqueous two-phase systems enable multiplexing of homogeneous immunoassays
Date:7/16/2014

A new protein biomarker test platform developed by researchers at the University of Michigan and Indiana University promises to improve diagnostic testing. The test can accurately and simultaneously measure multiple proteins that indicate the presence of diseases like graft-versus-host disease (bone marrow transplant rejection) in only two hours, no washing steps, and using only a minute volume of blood plasma. A report on this new technology can be found online in the journal TECHNOLOGY.

The protein test uses a micropatterning method developed in Shuichi Takayama's Micro/Nano/Molecular Biotechnology Lab. "Just as oil and water remain immiscible, we use two aqueous solutions that do not mix with each other," said Dr. Takayama, Professor of Biomedical Engineering and Macromolecular Science and Engineering. "Interestingly, these solutions can be patterned into arrays, whereas standard no-wash protein test reagents normally just mix together in solution. This novel capability makes it possible, for the first time, to measure multiple diagnostic proteins at a time in a no-wash format test."

To perform the assay, a few microliters of blood plasma is mixed with poly(ethylene glycol) and added to a microwell in a custom 384-well microplate. Next, microdroplets of dextran, containing complimentary pairs of antibody-beads, are dispensed into microbasins within the sample well. During a two-hour incubation, target plasma protein biomarkers diffuse from the poly(ethylene glycol) phase to the dextran droplets and become sandwiched by the antibody beads. The microplate is then read on a commercially available plate reader.

The team demonstrated the effectiveness of their bioassay by measuring protein biomarkers from cytokine-stimulated cells, as well as from the plasma of bone marrow transplant patients. Detecting levels of proinflammatory cytokines and chemokines in blood plasma can be crucial in the diagnosis of graft-versus-host disease (GVHD) - the leading cause of death among allogeneic bone marrow transplant patients.

"We envision that our user-friendly and highly accurate platform will be widely used by academic and clinical researchers for diagnostics as well as other applications," said Arlyne Simon, Ph.D., the lead author on this publication. "To ease the adoption of our technology into research and clinical labs, we designed custom microplates that can be analyzed by commercially available plate readers."


'/>"/>

Contact: Philly Lim
mllim@wspc.com
65-646-65775
World Scientific
Source:Eurekalert  

Related medicine news :

1. Is it time for regional cardiovascular emergency care systems across the US?
2. Program to coordinate regional systems to speed heart attack care
3. A systems approach to preventing obesity in early life
4. Researchers call for obesity prevention efforts to focus on community-wide systems
5. Cancers with disorganized traffic systems more difficult to treat: U of A research
6. Fitness 4 Home Online Introduces the Saber Strength Adjustable Weight Systems
7. Study looks at efforts to improve local food systems through policy
8. Systems biologist receives $2.5 million Pioneer Award for genome research
9. Students win $100K for 3-D printer to turn waste plastic into composting toilets, rainwater systems
10. In financial ecosystems, big banks trample economic habitats and spread fiscal disease
11. UCAN Selects Defran Systems to Support its Wide Range of Youth and Family Programs
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Aqueous two-phase systems enable multiplexing of homogeneous immunoassays
(Date:5/24/2016)... ... May 24, 2016 , ... Women's ... , Roberta Jordan is a Certified Nurse Midwife who was born and raised ... then went on to complete her masters degree from the University of Mexico. ...
(Date:5/24/2016)... ... 24, 2016 , ... Educational opportunities, and therefore life chances, ... communities providing richer opportunities. Recognizing the key role of housing in this system, ... school choice policies; (c) school desegregation policies; (d) wealth-focused policies; and (e) housing-focused ...
(Date:5/24/2016)... ... 24, 2016 , ... Regenerative Medicine is being transformed by ongoing research and ... protocols and patient results as have been achieved with Okyanos Cell Therapy ... of care for patients worldwide. , As the Medical Advisory Chairman at Okyanos, Eric ...
(Date:5/24/2016)... ... 2016 , ... Backed by decades of experience, Metroloji Okulu ... the best suited solution to meet regulatory requirements. Their professional staff also performs ... Istanbul. , Metroloji Okulu specializes in MadgeTech’s line of medical and pharmaceutical ...
(Date:5/24/2016)... ... May 24, 2016 , ... American Gene Technologies ... to its board of directors. Otterstatter is co-founder, president and CEO of ... technological innovations that lead to broad-based healthcare solutions. , “Jon knows how to ...
Breaking Medicine News(10 mins):
(Date:5/24/2016)... , May 24, 2016 ... beide primären Endpunkte und demonstriert ... in ‚ausgezeichneter plus guter , ...    ,      (Logo: http://photos.prnewswire.com/prnh/20130829/633895-a ... heute neue positive Daten von der MORA-Studie der ...
(Date:5/24/2016)... LAWRENCE, Mass. , May 24, 2016  NxStage ... medical technology company focused on advancing renal care, today ... Officer, plans to participate in the following schedule of ... will be made available at http://ir.nxstage.com/ . ... Jefferies Healthcare Conference NY, NY           Friday, ...
(Date:5/24/2016)... , May 24, 2016 ... de doble terapia del mundo, introduce catéteres para ... OrbusNeich, una compañía global especializada en ... vidas, ha expandido su cartera incluyendo productos para ... JADE™ y Scoreflex™ PTA son los dispositivos de ...
Breaking Medicine Technology: