Navigation Links
Aqueous two-phase systems enable multiplexing of homogeneous immunoassays
Date:7/16/2014

A new protein biomarker test platform developed by researchers at the University of Michigan and Indiana University promises to improve diagnostic testing. The test can accurately and simultaneously measure multiple proteins that indicate the presence of diseases like graft-versus-host disease (bone marrow transplant rejection) in only two hours, no washing steps, and using only a minute volume of blood plasma. A report on this new technology can be found online in the journal TECHNOLOGY.

The protein test uses a micropatterning method developed in Shuichi Takayama's Micro/Nano/Molecular Biotechnology Lab. "Just as oil and water remain immiscible, we use two aqueous solutions that do not mix with each other," said Dr. Takayama, Professor of Biomedical Engineering and Macromolecular Science and Engineering. "Interestingly, these solutions can be patterned into arrays, whereas standard no-wash protein test reagents normally just mix together in solution. This novel capability makes it possible, for the first time, to measure multiple diagnostic proteins at a time in a no-wash format test."

To perform the assay, a few microliters of blood plasma is mixed with poly(ethylene glycol) and added to a microwell in a custom 384-well microplate. Next, microdroplets of dextran, containing complimentary pairs of antibody-beads, are dispensed into microbasins within the sample well. During a two-hour incubation, target plasma protein biomarkers diffuse from the poly(ethylene glycol) phase to the dextran droplets and become sandwiched by the antibody beads. The microplate is then read on a commercially available plate reader.

The team demonstrated the effectiveness of their bioassay by measuring protein biomarkers from cytokine-stimulated cells, as well as from the plasma of bone marrow transplant patients. Detecting levels of proinflammatory cytokines and chemokines in blood plasma can be crucial in the diagnosis of graft-versus-host disease (GVHD) - the leading cause of death among allogeneic bone marrow transplant patients.

"We envision that our user-friendly and highly accurate platform will be widely used by academic and clinical researchers for diagnostics as well as other applications," said Arlyne Simon, Ph.D., the lead author on this publication. "To ease the adoption of our technology into research and clinical labs, we designed custom microplates that can be analyzed by commercially available plate readers."


'/>"/>

Contact: Philly Lim
mllim@wspc.com
65-646-65775
World Scientific
Source:Eurekalert  

Related medicine news :

1. Is it time for regional cardiovascular emergency care systems across the US?
2. Program to coordinate regional systems to speed heart attack care
3. A systems approach to preventing obesity in early life
4. Researchers call for obesity prevention efforts to focus on community-wide systems
5. Cancers with disorganized traffic systems more difficult to treat: U of A research
6. Fitness 4 Home Online Introduces the Saber Strength Adjustable Weight Systems
7. Study looks at efforts to improve local food systems through policy
8. Systems biologist receives $2.5 million Pioneer Award for genome research
9. Students win $100K for 3-D printer to turn waste plastic into composting toilets, rainwater systems
10. In financial ecosystems, big banks trample economic habitats and spread fiscal disease
11. UCAN Selects Defran Systems to Support its Wide Range of Youth and Family Programs
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Aqueous two-phase systems enable multiplexing of homogeneous immunoassays
(Date:2/23/2017)... ... February 23, 2017 , ... Rare Disease Report®, which ... participating in Rare Disease Day events, hosted by the Rare Disease Legislative Advocates ... a website, weekly e-newsletter and quarterly publication, will be conducting interviews with patients ...
(Date:2/23/2017)... ... February 23, 2017 , ... Los Angeles-based weight loss surgeon Michael ... “Mama June: From Not to Hot,” which will begin airing on February 24, 2017. ... to millions from the 2012 reality television series, “Here Comes Honey Boo Boo.” The ...
(Date:2/23/2017)... , ... February 23, 2017 , ... ... thought leadership , media relations, content marketing, social media management, corporate communications, SEO ... already in the state and in nearby New Hampshire, Massachusetts and Canada, Rosica ...
(Date:2/23/2017)... ... February 23, 2017 , ... ... will host a diverse symposium on “Doping in Sport: How the Culture ... Mullin Richter & Hampton LLP. The symposium will be held at Pepperdine University ...
(Date:2/23/2017)... ... February 23, 2017 , ... Pink Pig Publishing ... generations converge and explore the world from different perspectives. By providing a place ... to gain understanding, increase empathy, and find greater happiness. , "Our approach ...
Breaking Medicine News(10 mins):
(Date:2/24/2017)... CUPERTINO, Calif. , Feb. 24, 2017  In ... fourth quarter 2016 financial results press release, you are ... be broadcast live over the internet on Tuesday, March ... Pacific Time). A live audio webcast of ... at www.durect.com and clicking "Investor Relations."  If ...
(Date:2/24/2017)... , Feb. 23, 2017 The U.S. ... the PhenoTest BC Kit, performed on the Pheno ... organisms that cause bloodstream infections and provide information ... respond to (antibiotic sensitivity). The test also reduces ... this important information, which can guide antibiotic treatment ...
(Date:2/24/2017)... ITL Limited, ( ASX : ITD ), une société de ... excellents résultats semestriels clos le 31 décembre 2016 par ... « Résultats et mise à jour sur la croissance biomédicale ... Faits marquants Bénéfice ... hausse de 104 %) Bénéfice par action ...
Breaking Medicine Technology: