Navigation Links
Aqueous two-phase systems enable multiplexing of homogeneous immunoassays
Date:7/16/2014

A new protein biomarker test platform developed by researchers at the University of Michigan and Indiana University promises to improve diagnostic testing. The test can accurately and simultaneously measure multiple proteins that indicate the presence of diseases like graft-versus-host disease (bone marrow transplant rejection) in only two hours, no washing steps, and using only a minute volume of blood plasma. A report on this new technology can be found online in the journal TECHNOLOGY.

The protein test uses a micropatterning method developed in Shuichi Takayama's Micro/Nano/Molecular Biotechnology Lab. "Just as oil and water remain immiscible, we use two aqueous solutions that do not mix with each other," said Dr. Takayama, Professor of Biomedical Engineering and Macromolecular Science and Engineering. "Interestingly, these solutions can be patterned into arrays, whereas standard no-wash protein test reagents normally just mix together in solution. This novel capability makes it possible, for the first time, to measure multiple diagnostic proteins at a time in a no-wash format test."

To perform the assay, a few microliters of blood plasma is mixed with poly(ethylene glycol) and added to a microwell in a custom 384-well microplate. Next, microdroplets of dextran, containing complimentary pairs of antibody-beads, are dispensed into microbasins within the sample well. During a two-hour incubation, target plasma protein biomarkers diffuse from the poly(ethylene glycol) phase to the dextran droplets and become sandwiched by the antibody beads. The microplate is then read on a commercially available plate reader.

The team demonstrated the effectiveness of their bioassay by measuring protein biomarkers from cytokine-stimulated cells, as well as from the plasma of bone marrow transplant patients. Detecting levels of proinflammatory cytokines and chemokines in blood plasma can be crucial in the diagnosis of graft-versus-host
'/>"/>

Contact: Philly Lim
mllim@wspc.com
65-646-65775
World Scientific
Source:Eurekalert  

Page: 1 2

Related medicine news :

1. Is it time for regional cardiovascular emergency care systems across the US?
2. Program to coordinate regional systems to speed heart attack care
3. A systems approach to preventing obesity in early life
4. Researchers call for obesity prevention efforts to focus on community-wide systems
5. Cancers with disorganized traffic systems more difficult to treat: U of A research
6. Fitness 4 Home Online Introduces the Saber Strength Adjustable Weight Systems
7. Study looks at efforts to improve local food systems through policy
8. Systems biologist receives $2.5 million Pioneer Award for genome research
9. Students win $100K for 3-D printer to turn waste plastic into composting toilets, rainwater systems
10. In financial ecosystems, big banks trample economic habitats and spread fiscal disease
11. UCAN Selects Defran Systems to Support its Wide Range of Youth and Family Programs
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Aqueous two-phase systems enable multiplexing of homogeneous immunoassays
(Date:7/31/2015)... ... ... Precision Aesthetics is New York’s only Thermage skin tightening pinnacle and black diamond award ... fat reduction in New York. Due to its unparalleled level of experience for ... patients travel to New York to get their non-surgical skin tightening and body shaping ...
(Date:7/31/2015)... ... July 31, 2015 , ... The 2nd annual Ride to ... Downtown Memphis on October 3, 2015. The Ride to Fight On is a cycling ... between Methodist Healthcare, The West Clinic and The University of Tennessee Health Science Center. ...
(Date:7/31/2015)... ... July 31, 2015 , ... ... at its new True North Conference Center. The medical community, social workers, law ... community may be more aligned in the effort to better understand and combat ...
(Date:7/31/2015)... ... July 31, 2015 , ... M3 USA MDLinx.com, source ... launch three new Board Exam Courses. , The additions of Gastroenterology, Pulmonology and ... Prep from MDLinx makes preparing for the exam easy and painless. MDLinx offers ...
(Date:7/31/2015)... ... July 31, 2015 , ... According to an article published ... if they could reach a consensus on the steps that need to be taken ... group reached 90 percent consensus on a series of steps that each surgeon must ...
Breaking Medicine News(10 mins):Health News:West Cancer Center Announces 2nd Annual Ride to Fight On 2Health News:West Cancer Center Announces 2nd Annual Ride to Fight On 3Health News:Lakeview Health Links Sex Trafficking and Addiction in Special Jacksonville Educational Workshop 2Health News:Lakeview Health Links Sex Trafficking and Addiction in Special Jacksonville Educational Workshop 3Health News:MDLinx Offers Three New Board Exam Prep Courses - Gastroenterology, Pulmonology and Allergy/Immunology 2Health News:MDLinx Offers Three New Board Exam Prep Courses - Gastroenterology, Pulmonology and Allergy/Immunology 3Health News:Article on Plastic Surgery Consensus Highlights the Duties of Veteran Plastic Surgeons, says Beverly Hills Physicians 2Health News:Article on Plastic Surgery Consensus Highlights the Duties of Veteran Plastic Surgeons, says Beverly Hills Physicians 3
... Patricia Raymond offers tips on controlling your flatulence , ... ... is normal and necessary. In fact, the average person passes gas ... three liters daily). However, every body behaves differently and some experience ...
... program provides clients unparalleled live support services for http://www.appointment-plus.com ... ... Scottsdale, AZ (PRWEB) October 8, 2008 -- Appointment-Plus, the ... software client base by bucking trends. In a time ...
... today the extension of its existing strategic alliance with Nano-Terra, Inc., ... ... Germany (Business Wire EON) October 7, 2008 -- The original ... innovative, nanotechnology-based products and solutions based on Merck,s materials. , , ...
... IPRO, one of the,nation,s leading quality ... Liability Company (LLC) that will assume all ... with managed care,organizations (MCOs). The new entity, ... in New York State., (Logo: ...
... CHADD, the nation,s,largest organization serving people ... Annual,International Conference on AD/HD November 12-15 ... some of the leading experts speaking ... adults, clinicians, professionals, and,educators. To view ...
... Getting out of bed sooner helped quality of life after ... The best medicine for intensive care unit (ICU) patients may ... as they,re able, a new report says. , Based ... School of Medicine researchers concluded that the routine use of ...
Cached Medicine News:Health News:Managing Gas Isn't Rocket Science 2Health News:Managing Gas Isn't Rocket Science 3Health News:Appointment-Plus Continues Innovation With Technical Support Program 2Health News: Merck KGaA and Nano-Terra Announce Extension of Nanotechnology Solutions Alliance 2Health News: Merck KGaA and Nano-Terra Announce Extension of Nanotechnology Solutions Alliance 3Health News:IPRO Spins Off Managed Care Review Affiliate 2Health News:Early Mobility Best for ICU Patients 2
(Date:7/31/2015)... CHICAGO , July 31, 2015 ... Composition Analyzers Market by Product (Bioimpedance Analyzers (BIA), Dual-Energy ... and Hydrostatic Weighing) - Global Analysis & Forecast to ... Market was pegged at $911.1 Million 2014 and is ... at a CAGR of 9.4% from 2014 to 2019. ...
(Date:7/30/2015)... July 30, 2015  ResMed (NYSE: ... a definitive agreement to acquire Curative Medical, ... and sleep-disordered breathing medical devices and accessories. ... in China, combined with ResMed,s global leadership in sleep and respiratory ... China suffering from sleep-disordered ...
(Date:7/30/2015)... IRVINE, Calif. , July 30, 2015 /PRNewswire/ ... Novartis /Alcon executives have recently contacted the Company ... will begin as early as Monday, August 3, ... been quoted as saying that the coming electronic ... billions of dollars over the next several years.  ...
Breaking Medicine Technology:Body Composition Analyzers Market Worth $1,425.7 Million by 2019 2Body Composition Analyzers Market Worth $1,425.7 Million by 2019 3Body Composition Analyzers Market Worth $1,425.7 Million by 2019 4ResMed Invests in High-Growth Chinese Sleep and Respiratory Care Market with Curative Medical 2ResMed Invests in High-Growth Chinese Sleep and Respiratory Care Market with Curative Medical 3
... Maine, July 14, 2011 IDEXX Laboratories, Inc. (Nasdaq: ... its 2011 second quarter financial results for Friday, July ... conduct an analyst conference call beginning at 9:00 a.m. ... Individuals can access a live Webcast of the ...
... Biomedical Corporation (OTCBB:QTXB), an emerging leader in the ... on its patented technology platforms for the worldwide ... the lawsuit with NURX, its prior Joint Venture ... operations to expand its over-the-counter products and laboratory ...
Cached Medicine Technology:QuantRx Biomedical Settles Suit: Launches New PAD-Based Genomic Diagnostic Products 2QuantRx Biomedical Settles Suit: Launches New PAD-Based Genomic Diagnostic Products 3
... Laboratories is a national reference laboratory and ... and development. ARUP offers an extensive test ... tests in clinical and anatomic pathology. Owned ... clients include more than half of the ...
... Laboratories is a national reference laboratory and ... and development. ARUP offers an extensive test ... tests in clinical and anatomic pathology. Owned ... clients include more than half of the ...
... is a national reference laboratory and a ... development. ARUP offers an extensive test menu ... in clinical and anatomic pathology. Owned by ... include more than half of the nation's ...
... ARUP Laboratories is a national reference laboratory ... research and development. ARUP offers an extensive ... medical tests in clinical and anatomic pathology. ... Laboratories' clients include more than half of ...
Medicine Products: