Navigation Links
Anatomy of an outbreak
Date:5/5/2011

GALVESTON, Texas What causes a virus to suddenly begin infecting large numbers of people?

Scientists have long known that the process they call "viral emergence" involves a wide variety of factors. Some are changes in the environment, either generated by natural causes or human activity. Others are internal, arising from accidental changes mutations in the virus' genetic code.

Studying such mutations in different strains of the chikungunya virus has helped University of Texas Medical Branch researchers solve one of the most puzzling mysteries of chikungunya's emergence in Asia. They describe their results in an article in the online "Early Edition" section of the Proceedings of the National Academy of Sciences.

Chikungunya, which originated in Africa, is carried by mosquitoes and causes intensely painful arthritis that can last for months or years. Thanks to a discovery made at UTMB, scientists know that the virus' rapid spread was launched by a single mutation in an African strain of the virus.

The alteration was so small a single amino acid change in one of the virus' exterior "envelope" proteins that a researcher compared it to "a single missing comma in a six-page short story." But this so-called "E1-A226V mutation" made it possible for the virus to efficiently infect Aedes albopictus, a species of mosquito found nearly worldwide.

The mutated strain of the virus took full advantage of its new host, infecting millions of people as it spread across India, Thailand and Malaysia. It even jumped to northern Italy, carried by an infected traveler, where it established itself in the local Aedes albopictus mosquito population.

This albopictus-adapted strain's success raised a fundamental question, for this was not chikungunya's first visit to Asia. Strains of the virus transmitted by another mosquito species, Aedes aegypti, have caused sporadic outbreaks there for nearly six decades. If the virus was changing all the time, and only one minor mutation was necessary to switch the virus from aegytpi to albopictus a more widespread vector why hadn't that mutation happened in the strains that had arrived in Asia 60 years before?

"Asia is Aedes albopictus' native territory, but we can't find any evidence of chikungunya transmission by albopictus until the arrival of this new strain," said UTMB pathology professor Scott Weaver, senior author on the PNAS paper. "It was surprising to us that strains of this apparently very adaptable virus circulated in Asia for 60 years without making the adaptation that would allow them to be transmitted by albopictus."

To find out what was going on, Weaver and his colleagues lead author and postdoctoral fellow Konstantin Tsetsarkin, postdoctoral fellow Rubing Chen, research technician Grace Leal, assistant professor Naomi Forrester, professor Stephen Higgs, and research associate Jing Huang conducted experiments based on the hypothesis that some part of the Asian chikungunya strains' genetic code was suppressing the key mutation whenever it occurred and thus keeping it from infecting Aedes albopictus.

Using two different Asian strains into which they inserted the E1-A226V mutation, they systematically added additional genetic portions from the African strain, followed by specific mutations to determine which interacted with E1-A226V. Then they tested each change to see whether it affected Aedes albopictus infectivity.

Ultimately, they found that a single genetic element which also changed an amino acid in the same envelope protein altered by the E1-A226V mutation increased the Asian chikungunya strains' ability to infect Aedes albopictus by a hundredfold.

"This old Asian lineage needs an additional mutation to adapt to Aedes albopictus, and we think that's what protected India and Southeast Asia from much larger epidemics over the last 60 years," Weaver said. "But some African strains only needed one mutation to spark much larger outbreaks. And now, a strain that emerged from Africa in 2004 seems to be displacing the old Asian strains wherever it goes."

The chikungunya story, Weaver said, demonstrates how small genetic differences among viruses can have dramatic and unexpected effects on their ability to cause human disease. This study also will allow researchers to predict the amount of disease chikungunya may cause if it becomes endemic in the Americas.

"We don't really have the ability to predict when these viruses are going to suddenly mutate and change from one host to another. We can figure out how it happened retrospectively, but we have no idea how many near misses there are," Weaver said. "This was an opportunity to understand one situation where for a long time epidemic emergence didn't happen for a virus in Asia, and how it did happen suddenly when another strain got loose from Africa and started spreading around the globe."


'/>"/>

Contact: Jim Kelly
jpkelly@utmb.edu
409-772-8791
University of Texas Medical Branch at Galveston
Source:Eurekalert

Related medicine news :

1. Those To-Die-For High Heels May Alter Anatomy
2. Olsen, Drake, Schoenwolf receive highest honors from anatomy society
3. Vaccine Not Fail-Safe in Ongoing Mumps Outbreak
4. Dry Weather Boosts Odds of Flu Outbreaks
5. Massive Food Flavoring Recall Underscores Need for New Tracking Technology to Contain Outbreaks of Foodborne Illness
6. Preventive behaviors limited household transmission of H1N1 influenza during initial outbreak
7. Measles Outbreak Triggered by Unvaccinated Child
8. £3M awarded for climate model to predict disease outbreak
9. Food Safety Law Firm Investigates Major Outbreak of E. coli Linked To Lettuce
10. Jill Greenfield Comments on the Numerous Failings Highlighted by the Griffin Inquiry into Godstone Farm E.Coli 0157 Outbreak
11. Employers took many measures to protect employees and avoid business impact of H1N1 flu outbreak
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/8/2016)... Francisco, CA (PRWEB) , ... February 08, 2016 , ... ... raise $792,000 to help combat pancreatic cancer. , Gary D. Radine, who recently retired ... also was the American Cancer Society’s 2015 CEO of the Year , helped ...
(Date:2/8/2016)... ... February 08, 2016 , ... ... of organizations. DocuSyst provides a cloud hosted environment for FileHold software that ... integration with various 3rd party applications using the FileHold web services API. DocuSyst ...
(Date:2/8/2016)... ... February 08, 2016 , ... TopConsumerReviews.com recently awarded their ... removal products. , Moles are derived from a cluster of melanin when exposed to ... wrong places and create a lifetime of embarrassment. Historically, mole removal has ...
(Date:2/8/2016)... ... February 08, 2016 , ... According to ... the U.S. require dental technicians to be certified or obtain continuing education. To ... created the “What’s In Your Mouth?” campaign to inform dentists and patients about ...
(Date:2/7/2016)... ... February 07, 2016 , ... ... has added Kybella® to his medical and surgical expertise. Technically known as deoxycholic ... injectable medication used as a non-surgical alternative for reduction of fat below the ...
Breaking Medicine News(10 mins):
(Date:2/8/2016)... India , February 8, 2016 ... a new market research report "Ablation Technologies Market by ... (Csardiovascular, Cancer, Pain Management, Cosmetic Surgery, Ophthalmology, Gynecology) - ... studies the global market over the forecast period of ... $4.44 Billion by 2020, at CAGR of 10.5% from ...
(Date:2/8/2016)... Kan. , Feb. 8, 2016 /PRNewswire/ ... organization that formerly specialized in the development ... recently announced that it has divided its ... Capital. While Nueterra Capital will continue the ... services, NueHealth will operate a national system ...
(Date:2/8/2016)... , Feb. 8, 2016  A research team ... scientists has discovered details of how the abnormal breakage ... a particularly aggressive form of acute lymphoblastic leukemia (ALL). ... which genetic mutations trigger overproduction of immature cells, called ... The discoveries of the malfunction underlying the type called ...
Breaking Medicine Technology: