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Analyzing living cells quickly and accurately
Date:5/5/2014

The Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB in Stuttgart can now analyze living cells quickly and accurately with the help of Raman spectroscopy. The non-invasive optical procedure, which recognizes the molecular fingerprint of different materials, has primarily been employed in quality control for medications and pharmaceutical substances. Now biologists and biomedical researchers can also use this technology thanks to the research work at IGB. The technology is suited to investigating living cells without invasive techniques or altering them with dyes. In order to characterize stem cells or identify changes to tissues that are caused by tumors, inflammations, fungi, or bacteria, it is now sufficient to determine the individual cells' Raman spectrum a specialized energy spectrum having particular analytical capability,

"Under joint projects with universities, industrial partners, and the State of Baden-Wrttemberg, IGB has developed comprehensive know-how in this area over the last years and has advanced the technology from use in pure research to industrial implementation. We can now investigate not just individual cells in this manner, but entire tissue structures and organs. Next we want to further refine the technology and develop more applications," says Prof. Katja Schenke-Layland from IGB.

The unmistakable Raman spectrum

Cell biologists at IGB use a specially developed Raman spectroscope jointly designed and built with physicists at the Fraunhofer Institute for Physical Measurement Techniques IPM in Freiburg, Germany. The device is compact and can be conveniently used to investigate a wide range of scientific problems. The scientists are accumulating the spectra they have recorded into a database. "Each cell has a unique, unmistakable Raman spectrum. Doctors are able to compare the sample from their patients' cells with our data base and complete the diagnosis more quickly," says Schen
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Contact: Dr. Katja Schenke-Layland
katja.schenke-layland@igb.fraunhofer.de
49-711-970-4082
Fraunhofer-Gesellschaft
Source:Eurekalert  

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