Navigation Links
Analyzing living cells quickly and accurately

The Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB in Stuttgart can now analyze living cells quickly and accurately with the help of Raman spectroscopy. The non-invasive optical procedure, which recognizes the molecular fingerprint of different materials, has primarily been employed in quality control for medications and pharmaceutical substances. Now biologists and biomedical researchers can also use this technology thanks to the research work at IGB. The technology is suited to investigating living cells without invasive techniques or altering them with dyes. In order to characterize stem cells or identify changes to tissues that are caused by tumors, inflammations, fungi, or bacteria, it is now sufficient to determine the individual cells' Raman spectrum a specialized energy spectrum having particular analytical capability,

"Under joint projects with universities, industrial partners, and the State of Baden-Wrttemberg, IGB has developed comprehensive know-how in this area over the last years and has advanced the technology from use in pure research to industrial implementation. We can now investigate not just individual cells in this manner, but entire tissue structures and organs. Next we want to further refine the technology and develop more applications," says Prof. Katja Schenke-Layland from IGB.

The unmistakable Raman spectrum

Cell biologists at IGB use a specially developed Raman spectroscope jointly designed and built with physicists at the Fraunhofer Institute for Physical Measurement Techniques IPM in Freiburg, Germany. The device is compact and can be conveniently used to investigate a wide range of scientific problems. The scientists are accumulating the spectra they have recorded into a database. "Each cell has a unique, unmistakable Raman spectrum. Doctors are able to compare the sample from their patients' cells with our data base and complete the diagnosis more quickly," says Schenke-Layland.

The technology is already being employed on a practical basis by industrial partners. The scientists are working at present on a rapid test for cancer diagnosis. "Doctors using mobile Raman spectroscopes during an operation could unambiguously say whether the patient has cancer or not simply by comparing the cell sample with the data base," according to Schenke-Layland.

Cancer diagnoses are still complicated and prolonged. After excising the tissue for biopsy, it first must be prepared for further analysis for example by suitably sectioning or dying it to identify biomarkers. "But this always requires intervention in the specimen and manipulating it in some way," according to Schenke-Layland.

The specimen is then transferred to a pathologist who analyses whether the tissue contains malignant or benign cells. This method is error prone and can lead to the specimen being unusable for other tests in the end. "Human error is reduced by a software-based comparison with our data base," according to Schenke-Layland.

Employment in cancer diagnostics and regenerative medicine

There are numerous additional applications for this non-invasive optical technology especially in regenerative medicine. Artificially grown tissue may replace diseased cells in patients and thereby help the tissue to heal. To accomplish this, tissue-specific cells from bone marrow, for instance, must be removed and the stem cells extracted. Bone marrow is made up of highly diverse cells and it is complicated to differentiate the adult stem cells from the ordinary tissue cells. In addition, the stem cells must be 100% correctly identified and separated. If this does not happen and other types of cells are cultured into the implant, the body may not react as hoped, causing implant rejection or tumor formation.

Raman spectroscopy is a process to unambiguously identify and differentiate various materials from one another. It is based on the interaction of electromagnetic radiation and matter. If matter is irradiated with light at a precisely defined frequency, some of the photons of this light interact with the molecules of the matter, which thereby shifts the photons' own energy spectrum. This frequency shift of the laser light, also known as inelastic optical scattering, is recorded by the Raman spectroscope. The effect is named after the Indian physicist C. V. Raman, who received the 1930 Nobel Prize in Physics for his work. The frequency shifts are a function of the material and every material possesses an unmistakable spectral fingerprint.


Contact: Dr. Katja Schenke-Layland

Related medicine news :

1. First validated method for analyzing flavanols and procyanidins in cocoa products could help scientists and the industry in standardized reporting
2. Analyzing the Facebook Effect on organ and tissue donation
3. Donated time, a tool for analyzing housework and care
4. Analyzing Data to Improve Clinical, Quality, and Process Improvement in the Era of Payment Reform
5. Test Your Hair Launches New Indiegogo Campaign to Promote Awareness of Hair Mineral Analysis, the Scientific and Reliable Method of Analyzing Your Health
6. White Women Make Up Bulk of Assisted-Living Residents
7. Living Near Major Roads May Shorten Heart Attack Survival
8. Heart attack survivors living close to highways face higher 10-year death risk
9. Living longer - variability in infection-fighting genes can be a boon for male survival
10. Cash, Coaching May Boost Healthy Living
11. Loneliness, Living Alone Might Shorten Life
Post Your Comments:
Related Image:
Analyzing living cells quickly and accurately
(Date:11/27/2015)... ... 2015 , ... "When I was traveling, I was very ... "Many people catch diseases simply from sitting on such dirty toilet seats. I ... germs." , He developed the patent-pending QUDRATECS to eliminate the need to sit ...
(Date:11/27/2015)... Aliso Viejo, CA (PRWEB) , ... November 27, 2015 , ... ... exclusively for use in Final Cut Pro X. With ProSidebar: Fasion, video editors ... banners, or use ProSidebar as a minimalist title opener. Utilize presets featuring self-animating ...
(Date:11/27/2015)... ... November 27, 2015 , ... ... Microsoft Dynamics SL User Group (MSDSLUG). Recognized as Microsoft’s official group for end ... Microsoft Dynamics SL software users, partners, industry experts and representatives. Intellitec Solutions’ membership ...
(Date:11/27/2015)... (PRWEB) , ... November 27, 2015 , ... ... Health-E-minds, an innovative online platform for mental health and wellness consultation, has collaborated ... This partnership will bridge the knowledge gap experienced by parents and bring advice ...
(Date:11/27/2015)... , ... November 27, 2015 , ... Dr. Thomas ... Associates, Inc. and Dr. Tucker Bierbaum with Emergency Medicine at St., Joseph ... that both STEMI and Sepsis conditions present in similar ways and require time-critical intervention ...
Breaking Medicine News(10 mins):
(Date:11/26/2015)... 26, 2015 Research and Markets ( ) ... Pacemaker Market Outlook to 2019 - Rise in Cardiac Disorders ... report to their offering. Boston ... Boston scientific and others. --> ... Biotronik, Boston scientific and others. ...
(Date:11/26/2015)... , November 26, 2015 ... addition of the "2016 Future Horizons ... of Abuse Testing Market: Supplier Shares, Country ... report to their offering. --> ... the "2016 Future Horizons and Growth ...
(Date:11/26/2015)... November 26, 2015 ... universitetssjukhus ser potential att använda SyMRI för ... för patienter med multipel skleros (MS) ... med SyntheticMR AB för att kunna använda ... sjukhuset. Med SyMRI kan man generera flera ...
Breaking Medicine Technology: