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American Chemical Society podcast: The first caffeine-'addicted' bacteria
Date:6/11/2013

The latest episode in the American Chemical Society's (ACS') award-winning Global Challenges/Chemistry Solutions podcast series describes bacteria that are "addicted" to caffeine in a way that promises practical uses ranging from decontamination of wastewater to bioproduction of medications for asthma.

Based on a report by Jeffrey Barrick, Ph.D., and colleagues in the journal ACS Synthetic Biology, the new podcast is available without charge at iTunes and from http://www.acs.org/globalchallenges.

Some people may joke about living on caffeine, but scientists now have genetically engineered E. coli bacteria to do that literally.

Barrick and colleagues note that caffeine and related chemical compounds have become important water pollutants due to widespread use in coffee, soda pop, tea, energy drinks, chocolate and certain medications. These include prescription drugs for asthma and other lung diseases.

The scientists knew that a natural soil bacterium, Pseudomonas putida CBB5, can actually live solely on caffeine and could be used to clean up such environmental contamination. So they set out to transfer genetic gear for breaking down caffeine from P. putida into that old workhorse of biotechnology, E. coli, which is easy to handle and grow.

The study reports their success in doing so, as well as use of the E. coli for decaffeination and measuring the caffeine content of beverages. It describes development of a synthetic packet of genes for breaking down caffeine and related compo
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Contact: Michael Bernstein
m_bernstein@acs.org
202-872-6042
American Chemical Society
Source:Eurekalert

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