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ATCC Examined the Importance of High-quality Controls in Assay Design and Development During the Association for Molecular Pathology (AMP) 2013 Annual Meeting
Date:11/25/2013

Manassas, VA (PRWEB) November 25, 2013

The use of well-defined controls in assay development is essential for determining the reliability and reproducibility of data obtained from molecular applications. ATCC spoke out on this topic during its first participation at the Corporate Workshop Day at the AMP 2013 Annual Meeting, emphasizing the importance of using authenticated and highly characterized biomaterials for assay development and verification. More than 75 researchers from pharmaceutical and molecular diagnostics companies attended the workshop. The presentation was divided into two parts, first focusing on controls for genetic mutations in cancer biology, followed by a discussion on the detailed characterization of ATCC infectious disease strains and nucleic acid controls.

Fang Tian, Ph.D., Lead Scientist for ATCC Cell Systems, led the discussion by describing the rapidly changing landscape of diagnostics, including the vast amount of human genetic data currently available via open-access. Moreover, with the development of smaller, faster, and more affordable sequencing technologies, investigators now require a stable and renewable source of controls, such as fully characterized and authenticated cell lines. To meet these needs, ATCC organized Tumor Cell Panels representing cell lines grouped by tissue type and annotated with published gene mutation data. Further, Dr. Tian explained, “ATCC is not only pairing cell lines with published gene mutation data, we are also performing extensive laboratory analyses to confirm the presence, stability, and expression of genetic mutations using tools like sequencing, PCR, and Western Blot.”

Liz Kerrigan, Director of Standards at ATCC noted, “There are a lot of choices for controls in research, but it’s imperative to obtain controls from a reliable source; knowing how they were produced and how identities were verified – that’s the importance of authentication.” ATCC
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