Navigation Links
A versatile mouse that can teach us about many diseases and drugs
Date:3/13/2014

Scientists from the UK and Australia have created a mouse that expresses a fluorescing 'biosensor' in every cell of its body, allowing diseased cells and drugs to be tracked and evaluated in real time and in three dimensions.

This biosensor mimics the action of a target molecule, in this case a protein known as 'Rac', which drives cell movement in many types of cancer. Rac behaves like a switch, oscillating on the molecular level between two states active or inactive.

When Rac is active, the biosensor picks up chemical cues and glows blue. When Rac is inactive the biosensor glows yellow.

Using sophisticated imaging techniques, it is possible to follow Rac activation in any organ at any time, or watch moment-by-moment oscillation of Rac activity at the front or back of cells as they move in the body. This technology has been used to monitor Rac activity in many organs in response to drug treatment.

The biosensor is a single molecule probe named 'Raichu-Rac' and was invented by Japanese scientist, Professor Miki Matsuda, in 2002.

Although many researchers have used Raichu-Rac since 2002, this is the first time a mouse has been genetically modified successfully to express the molecule throughout the body without affecting cell function. The mouse can be used to study any cancer type by crossing it with other models, limiting expression of Raichu-Rac to specific cell or tissue types. The mouse can also easily be adapted to study diseases other than cancer by expressing the biosensor in different disease models.

Dr Paul Timpson began the study with colleagues from the Beatson Institute for Cancer Research in Glasgow and completed it at Sydney's Garvan Institute of Medical Research. He collaborated closely throughout the process with Dr Heidi Welch from the Babraham Institute in Cambridge the creator of the mouse who uses it to study the movement of immune cells, known as neutrophils. The study, now online, has been published in the prestigious journal Cell Reports.

"The great thing about this mouse is its flexibility and potential for looking at a broad range of diseases and molecular targets," said Dr Paul Timpson.

"It allows us to watch and map, in real time, parts of a cell or organ where Rac is active and driving invasion. In cancers, a lot of blue indicates an aggressive tumour that is in the process of spreading."

"You can literally watch parts of a tumour turn from blue to yellow as a drug hits its target. This can be an hour or more after the drug is administered, and the effect can wane quickly or slowly. Drug companies need to know these details - specifically how much, how often and how long to administer drugs."

Dr Heidi Welch is very modest about her role in creating the mouse, viewing it mostly as a tool that will help other scientists to understand Rac and work out how to stop cancer cells from moving.

"The credit must go to Professor Miki Matsuda, the genius who invented the biosensor in the first place 12 years ago," she said.

"He made his discovery freely available to the scientific community, and has been very open about his findings since."

"Miki Matsuda was super-helpful in suggesting the expression levels we should be looking for, and in recommending the exact biosensor we should use, out of many he developed. He was superb."

According to Welch, competition is growing rapidly in this area, with Matsuda himself making biosensor mice for a variety of target molecules.


'/>"/>

Contact: Alison Heather
a.heather@garvan.org.au
61-292-958-128
Garvan Institute of Medical Research
Source:Eurekalert

Related medicine news :

1. Versatile microRNAs choke off cancer blood supply, suppress metastasis
2. Blade Runner Golf Announces a Tour Model Chipper That Is Versatile from 7 Iron Distances and Provides Success for Any Players Skill Level
3. Mouse Study Hints at New Path for Diabetes Treatment
4. This mousetrap may save lives
5. Its Not Just What You Eat, Its When You Eat, Mouse Study Finds
6. Belly Membrane May Regulate Immune System, Mouse Study Finds
7. Mouse Study Suggests Certain Fats Could Trigger Crohns, Colitis
8. New mouse model helps explain gene discovery in congenital heart disease
9. TB treatment paradox: Mouse studies show bodys own response helps TB bacteria survive
10. Scientists develop mouse model that could lead to new therapies for liver cancer
11. Mouse With Human-Like Immune System Could Advance AIDS Research
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/27/2016)... ... 27, 2016 , ... TopConsumerReviews.com recently awarded their highest five-star rating to Best ... individuals in the United States and Canada wear eyeglasses. Once considered to be a ... and make a fashion statement. Even celebrities use glasses as a way of creating ...
(Date:6/26/2016)... ... June 26, 2016 , ... Brent Kasmer, a legally blind and certified personal trainer ... through a fitness app. The fitness app plans to fix the two major problems leading ... a one size fits all type program , They don’t eliminate all the ...
(Date:6/25/2016)... ... , ... "With 30 hand-drawn hand gesture animations, FCPX users can easily customize ... Pixel Film Studios. , ProHand Cartoon’s package transforms over 1,300 hand-drawn pictures into ... Simply select a ProHand generator and drag it above media or text in the ...
(Date:6/25/2016)... ... June 25, 2016 , ... Conventional wisdom preaches the benefits of ... of the latter, setting the bar too high can result in disappointment, perhaps even ... progress toward their goal. , Research from PsychTests.com reveals that behind ...
(Date:6/24/2016)... ... June 24, 2016 , ... The Pulmonary ... Dallas that it will receive two significant new grants to support its work ... marked its 25th anniversary by recognizing patients, medical professionals and scientists for their ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... 24, 2016 According to a ... (Standard Pen Needles, Safety Pen Needles), Needle Length (4mm, ... Hormone), Mode of Purchase (Retail, Non-Retail) - Trends & ... studies the market for the forecast period of 2016 ... 2.81 Billion by 2021 from USD 1.65 Billion in ...
(Date:6/24/2016)... Belgium , June 24, 2016 ... today announced the appointment of Dr. Edward ... as a Non-Executive Director, effective June 23, 2016.Dr. ... Compensation and Nominations and Governance Committees.  As a ... will provide independent expertise and strategic counsel to ...
(Date:6/23/2016)...  MedSource announced today that it has selected ... of choice.  This latest decision demonstrates MedSource,s commitment ... clients by offering a state-of-the-art electronic data capture ... as the EDC platform of choice in exchange ... has long been a preferred EDC platform by ...
Breaking Medicine Technology: