Navigation Links
A novel in vitro model for light-induced wound healing
Date:3/4/2010

Washington, DC, USA Today, during the 39th Annual Meeting of the American Association for Dental Research, convening at the Walter E. Washington Convention Center in Washington, DC, lead researcher C. Millan (U.S. Army Dental Corps, Martinez, Georgia) will present a poster of a study titled "A Novel In Vitro Model for Light-Induced Wound Healing." Studies have suggested that exposure to minimal doses of blue-violet light (400-500 nm) elicits production of small amounts of reactive oxygen species (ROS), and contributes to increased mitochondrial activity and cell growth in epithelial cells. Many growth factor signaling pathways generate ROS.

In this study, Millan and other researchers involved in this study hypothesize that exposure to blue-violet light may enhance cell growth. To test this hypothesis, they developed a novel in-vitro wound healing model that allowed them to monitor the cellular responses to a single, small dose of light in cultured cells.

Normal human epidermal keratinocytes (NHEK) and human gingival fibroblasts (HGF) were plated around cloning cylinders. At confluency, the cylinders were removed to create a wound. Cells were treated with a single 5 J/cm2 light dose delivered by a quartz-tungsten-halogen light source. Mitochondrial succinate dehydrogenase activity was measured via a standard MTT assay, and cell proliferation was assessed using DRAQ5 DNA dye. Conditioned media were collected at each time point and used in a growth factor antibody array (RayBio) to compare the secretion products.

Growth factor array results showed that NHEK responded to blue light exposure by increasing secretion of several growth factors including insulin-like growth factor binding protein-1, amphiregulin, epidermal growth factor and fibroblast growth factor-b. Likewise, mitochondrial dehydrogenase activity and cell proliferation were enhanced in NHEK. In contrast, HGF did not respond to blue light exposure significantly in any of the parameters tested.

These results show that NHEK cells responded robustly to a single, small dose of light by increasing their mitochondrial activity, DNA synthesis, and production of growth factors. Together, these data suggest that blue light may be useful to enhance epithelial cell growth in a wound site.


'/>"/>

Contact: Ingrid Thomas
ithomas@aadronline.org
703-299-8084
International & American Association for Dental Research
Source:Eurekalert

Related medicine news :

1. Novel 3-D cell culture model shows selective tumour uptake of nanoparticles
2. Novel virus detection identifies new viruses in study of respiratory infections and asthma attacks
3. Researchers uncover novel mechanism that balances the sizes of functional areas in the brain
4. Novel Cooling Therapy May Have Aided Injured Football Player
5. ECLIPSE data on effects of Otsukas investigational novel treatment, Tolvaptan
6. SNM seeks novel approaches to molecular imaging to showcase at annual meeting
7. GrantAdler Shipping Novel Implantable Vascular Access Port
8. SNM seeks novel approaches to molecular imaging to showcase at annual meeting
9. Novel strategy under study for aggressive leukemia
10. ISTO Technologies Receives 510(k) Clearance for a Novel Bone Graft Substitute and Bone Graft Extender
11. 454 Sequencing(TM): Science Paper Describes a Novel, Highly Efficient Method of Sequencing Ancient DNA; Sequences the Mitochondria of 10 Individual Mammoths to Prove It
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/23/2017)... ... 23, 2017 , ... Yesterday, U.S. Senate Republicans revealed details ... and replace the Affordable Care Act (ACA). Like the bill narrowly passed by ... a public health insurance program for low-income children, pregnant women, parents of dependent ...
(Date:6/23/2017)... ... June 23, 2017 , ... The Military Officers Association of America (MOAA) ... of Veterans Affairs, retired Marine Col. Thomas G. Bowman. , Bowman currently serves as ... brings an intimacy with the issues and challenges veterans face with the VA. Following ...
(Date:6/23/2017)... ... June 23, 2017 , ... Military Connection friend and ... to the JFK Virgin Atlantic lounge. , Bensko is no stranger to the ... ago, Bensko dedicated her life to supporting our wounded veterans. A world-class photographer, her ...
(Date:6/23/2017)... DeSoto, Kan. (PRWEB) , ... June 23, 2017 , ... ... Kan. , The restaurant is located at 4210 SW 21st St. Topeka, Kan. ... Brandon Graham, owner of Graham Restaurant Group who owns three other locations in the ...
(Date:6/23/2017)... Washington, D.C. (PRWEB) , ... June 23, 2017 ... ... temperature during cooking is essential, and two new videos highlight the importance of ... Extension Specialist Christine Bruhn, Ph.D., who has done extensive research on consumer food ...
Breaking Medicine News(10 mins):
(Date:6/16/2017)... BOSTON , June 16, 2017  Exactly 50 years ... kicked off what later became known as the San Francisco ... www.northernlight.com ) is unveiling two radical innovations in strategic market ... summer.  This announcement marks the beginning of Northern Light,s "Summer ... ...
(Date:6/14/2017)... N.C. , June 14, 2017  In 2016, ... Creative Startups pitch competition and came away ... reality platform is described by Forbes as "entering the ... American Medical Association as teaching "empathy to medical professionals ... the startup was recently named a finalist for the ...
(Date:6/11/2017)... , June 10, 2017  Eli Lilly and Company (NYSE: ... three Phase 3 studies of galcanezumab, an investigational treatment ... late-breaking data on several key secondary endpoints for galcanezumab ... from these studies (EVOLVE-1, EVOLVE-2 and REGAIN) will be ... scientific meeting in Boston . ...
Breaking Medicine Technology: