Navigation Links
A novel in vitro model for light-induced wound healing
Date:3/4/2010

Washington, DC, USA Today, during the 39th Annual Meeting of the American Association for Dental Research, convening at the Walter E. Washington Convention Center in Washington, DC, lead researcher C. Millan (U.S. Army Dental Corps, Martinez, Georgia) will present a poster of a study titled "A Novel In Vitro Model for Light-Induced Wound Healing." Studies have suggested that exposure to minimal doses of blue-violet light (400-500 nm) elicits production of small amounts of reactive oxygen species (ROS), and contributes to increased mitochondrial activity and cell growth in epithelial cells. Many growth factor signaling pathways generate ROS.

In this study, Millan and other researchers involved in this study hypothesize that exposure to blue-violet light may enhance cell growth. To test this hypothesis, they developed a novel in-vitro wound healing model that allowed them to monitor the cellular responses to a single, small dose of light in cultured cells.

Normal human epidermal keratinocytes (NHEK) and human gingival fibroblasts (HGF) were plated around cloning cylinders. At confluency, the cylinders were removed to create a wound. Cells were treated with a single 5 J/cm2 light dose delivered by a quartz-tungsten-halogen light source. Mitochondrial succinate dehydrogenase activity was measured via a standard MTT assay, and cell proliferation was assessed using DRAQ5 DNA dye. Conditioned media were collected at each time point and used in a growth factor antibody array (RayBio) to compare the secretion products.

Growth factor array results showed that NHEK responded to blue light exposure by increasing secretion of several growth factors including insulin-like growth factor binding protein-1, amphiregulin, epidermal growth factor and fibroblast growth factor-b. Likewise, mitochondrial dehydrogenase activity and cell proliferation were enhanced in NHEK. In contrast, HGF did not respond to blue light exposure significantly in any of the parameters tested.

These results show that NHEK cells responded robustly to a single, small dose of light by increasing their mitochondrial activity, DNA synthesis, and production of growth factors. Together, these data suggest that blue light may be useful to enhance epithelial cell growth in a wound site.


'/>"/>

Contact: Ingrid Thomas
ithomas@aadronline.org
703-299-8084
International & American Association for Dental Research
Source:Eurekalert

Related medicine news :

1. Novel 3-D cell culture model shows selective tumour uptake of nanoparticles
2. Novel virus detection identifies new viruses in study of respiratory infections and asthma attacks
3. Researchers uncover novel mechanism that balances the sizes of functional areas in the brain
4. Novel Cooling Therapy May Have Aided Injured Football Player
5. ECLIPSE data on effects of Otsukas investigational novel treatment, Tolvaptan
6. SNM seeks novel approaches to molecular imaging to showcase at annual meeting
7. GrantAdler Shipping Novel Implantable Vascular Access Port
8. SNM seeks novel approaches to molecular imaging to showcase at annual meeting
9. Novel strategy under study for aggressive leukemia
10. ISTO Technologies Receives 510(k) Clearance for a Novel Bone Graft Substitute and Bone Graft Extender
11. 454 Sequencing(TM): Science Paper Describes a Novel, Highly Efficient Method of Sequencing Ancient DNA; Sequences the Mitochondria of 10 Individual Mammoths to Prove It
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/26/2016)... North Carolina (PRWEB) , ... June 26, 2016 , ... ... release of a new product that was developed to enhance the health of felines. ... for centuries. , The two main herbs in the PawPaws Cat Kidney Support ...
(Date:6/25/2016)... Austin, TX (PRWEB) , ... June 25, 2016 , ... ... Fellow of the American College of Mohs Surgery and to Dr. Russell Peckham for ... popular and highly effective treatment for skin cancer. The selective fellowship in Mohs Micrographic ...
(Date:6/25/2016)... ... June 25, 2016 , ... As a lifelong Southern ... Laude and his M.D from the David Geffen School of Medicine at UCLA. He ... Los Angeles to complete his fellowship in hematology/oncology at the UCLA-Olive View-Cedars Sinai program ...
(Date:6/24/2016)... ... June 24, 2016 , ... A recent article published June ... with. The article goes on to state that individuals are now more comfortable seeking ... common operations such as calf and cheek reduction. The Los Angeles area medical group, ...
(Date:6/24/2016)... ... 24, 2016 , ... The Pulmonary Hypertension Association (PHA) learned ... receive two significant new grants to support its work to advance research and ... by recognizing patients, medical professionals and scientists for their work in fighting pulmonary ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... , June 24, 2016  Consumers have taken ... regulators/payers have placed more emphasis on patient outcomes. ... support programs in the pharmaceutical industry have evolved ... Consequently, pharmaceutical companies are focusing on becoming more ... providing products and services that improve health. ...
(Date:6/24/2016)... 24, 2016  Global Blood Therapeutics, Inc. (GBT) (NASDAQ: ... novel therapeutics for the treatment of grievous blood-based ... closing of its previously announced underwritten public offering ... public offering price of $18.75 per share. All ... by GBT. GBT estimates net proceeds from the ...
(Date:6/24/2016)... Dublin ... of the " Global Markets for Spectroscopy Equipment" ... This report focuses on the global ... including its applications in various applications. The report deals ... three main industries: pharmaceutical and biotechnology, food and beverage, ...
Breaking Medicine Technology: