Navigation Links
A new light on tumor immunotherapy for gastric cancer
Date:12/23/2008

Dendritic cells (DCs) are professional antigen presenting cells (APCs) that both initiate and modulate the immune response. DCs are cells in the pathway of antigen capture and presentation to T cells, with the unique ability to directly prime nave CD4+ and CD8+ T cells. Gastric cancer is one of the most common cancers. Although gastric cancer therapy has made great progress, it is still difficult to treat advanced gastric cancer, as it has spread to the lymph glands and metastasized. Currently, tumor immunotherapy for gastric cancer has potential. DCs are believed to be essential for stimulating tumor-specific cytotoxic T lymphocyte (CTL) and inducing the protective and therapeutic anti-tumor immunity.

A research team led by Dr. Liang Wang from China investigated whether bone marrow-derived DCs (BM-DCs) pulsed with tumor lysates induce immunity against gastric cancer. Their results will be published on December 14, 2008 in the World Journal of Gastroenterology.

In this study, c-kit+ hematopoietic progenitor cells were magnetically isolated with a MiniMACS separator from BALB/c mice bone marrow cells. These cells were cultured with cytokines GM-CSF, IL-4, and TNF-alpha to induce their maturation. They were analysed by morphological observation, phenotype analysis, and mixed lymphocyte reaction (MLR). BM-DCs were pulsed with tumor cell lysate obtained by rapid freezing and thawing at a 1:3 DC:tumor cell ratio. Finally, CTL activity and interferon-gamma secretion was evaluated ex vivo

They found that c-kit+ hematopoietic progenitor cells from mice bone marrow cells cultured with cytokines for 8 d showed the character of typical mature DCs. However, immature DCs cultured with cytokines for 5 d did not have typical DCs phenotypic markers and could not stimulate allogeneic T cells. Ex vivo primed T cells with SGC-7901 tumor cell lysate-pulsed (TP) DCs were able to induce effective CTL activity against SGC-7901 tumor cells (E:T = 100:1, 69.55% 6.05% specific lysis), but not B16 tumor cells, and produced higher levels of IFN-gamma when stimulated with SGC-7901 tumor cells but not when stimulated with B16 tumor cells (1575.31 60.25 pg/mL in SGC-7901 group vs 164.11 18.52 pg/mL in B16 group, P < 0.01). These results indicated that BM-derived DCs pulsed with tumor lysates can induce anti-tumor immunity specific to gastric cancer ex vivo.


'/>"/>

Contact: Lai-Fu Li
wjg@wjgnet.com
0086-105-908-0039
World Journal of Gastroenterology
Source:Eurekalert

Related medicine news :

1. Guardian Life Flight, Air Ambulance Service Provider, Announces Addition of Sam Kini, M.D. To Its Team
2. Annual Congress European Association of Urology: highlights in Stockholm
3. Festival of Lights Holiday Celebration
4. Easter Seals Study Sheds New Light on Parents Life-Long Fears, Anxieties and Critical Supports Needed to Raise a Child With Autism
5. Electronic Sports Launches Dogfight Fitness Flight Simulator: The Next Generation of Exergaming
6. U.S. Supreme Court Ruling Allowing Light Cigarette Lawsuits Is Victory for Consumers and Health
7. New Report to Highlight Need for Public Health Insurance Plan Outlined in Obamas Proposals
8. Narrow-band imaging comparable to white light colonoscopy in differentiating colorectal polyps
9. Light shines for potential early cancer diagnosis technique
10. Sinobiopharma Reports Highlights of Third Quarter Results for Its Operating Subsidiary, Dong Ying China
11. Experts Highlight New Tool as a Timely Addition to Asthma Treatment
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/9/2016)... ... December 09, 2016 , ... Sober College, the game-changing ... grand opening of the Sober College Robert Pfeifer Memorial Learning Center at its ... and was attended by an overwhelming amount of alumni, family, colleagues and friends ...
(Date:12/9/2016)... ... December 09, 2016 , ... Mediaplanet today announces distribution of the latest edition ... to sign up as an organ donor for the 123,000 people in the United ... donor can save up to 8 saves through organ donation and enhance many others ...
(Date:12/9/2016)... ... December 09, 2016 , ... MEDI+SIGN®, a ... today that a new solution for Emergency Departments (ED) has been added to ... in Emergency Department examination rooms, and with a simplified pallet of information available ...
(Date:12/9/2016)... Harrisburg, PA (PRWEB) , ... December 09, 2016 ... ... concussion education program through the Pennsylvania Cable Network (PCN) during the summer of ... Brain Injury Implementation Grant provided by the United States Department of Health and ...
(Date:12/8/2016)... ... December 08, 2016 , ... The Florida ... Undersea and Hyperbaric Medical Society (UHMS), the leading authority in hyperbaric medicine. This ... Only a few hospitals and facilities have earned this distinction. This is the ...
Breaking Medicine News(10 mins):
(Date:12/9/2016)... DUBLIN , Dec 9, 2016 ... "High-Throughput Screening Market - Forecast to 2021" report to ... ... adoption of open innovative models in the pharmaceutical and biotechnology ... spending and rising government funding. Emerging markets and growing research ...
(Date:12/9/2016)... , Dec 9, 2016 Research and Markets ... 2016-2020" report to their offering. ... The global travel vaccines market to grow ... The report covers the present scenario and the growth prospects of ... size, the report considers the revenue generated from the sales of ...
(Date:12/8/2016)... LONDON , Dec. 8, 2016 Australia ... GlobalData,s new report, "Australia Ophthalmic Lasers Market Outlook to ... Lasers market. The report provides value, in millions of ... within market segements - Excimer Lasers, Femtosecond Lasers and ... and distribution shares data for each of these market ...
Breaking Medicine Technology: