Navigation Links
A molecular 'superglue' based on flesh-eating bacteria
Date:4/11/2013

NEW ORLEANS, April 11, 2013 In a classic case of turning an enemy into a friend, scientists have engineered a protein from flesh-eating bacteria to act as a molecular "superglue" that promises to become a disease fighter. And their latest results, which make the technology more versatile, were the topic of a report here today at the 245th National Meeting & Exposition of the American Chemical Society, the world's largest scientific society.

"We've turned the tables and put one kind of flesh-eating bacterium to good use," said Mark Howarth, Ph.D., who led the research. "We have engineered one of its proteins into a molecular superglue that adheres so tightly that the set-up we used to measure the strength actually broke. It resists high and low temperatures, acids and other harsh conditions and seals quickly. With this material we can lock proteins together in ways that could underpin better diagnostic tests for early detection of cancer cells circulating in the blood, for instance. There are many uses in research, such as probing how the forces inside cells change the biochemistry and affect health and disease."

Howarth's team at the University of Oxford in the United Kingdom genetically engineered the glue from a protein, FbaB, that helps Streptococcus pyogenes (S. pyogenes) bacteria infect cells. S. pyogenes is one of the microbes that can cause the rare necrotizing fasciitis, or flesh-eating bacteria syndrome, in which difficult-to-treat infections destroy body tissue.

They split FbaB into two parts, a larger protein and a smaller protein subunit, termed a peptide. Abbreviating S. pyogenes as "Spy," they named the small peptide "SpyTag" and the larger protein "SpyCatcher." The gluing action occurs when SpyTag and SpyCatcher meet. They quickly lock together by forming one of the strongest possible chemical bonds. SpyCatcher and SpyTag can be attached to the millions of proteins in the human body and other living things, thus gluing proteins together.

In an advance reported at the meeting, Howarth described how Jacob Fierer, a graduate student on the research team, greatly reduced the size of the SpyCatcher part of the technology. That achievement makes the technology more flexible, enabling scientists to connect proteins into new architectures, he said.

One of the applications on the horizon involves testing the technology as a new way to detect "circulating tumor cells," or CTCs. Tumors shed these cells into the bloodstream, where they may act as seeds, spreading or metastasizing cancer from the original site to other parts of the body. That spreading is the reason why cancer is such a serious health problem. Detecting CTCs is an active area of research worldwide because of its potential for early diagnosis of cancer from blood samples rather than biopsies and determining when new treatments may be needed to prevent the disease from spreading.

Howarth said that the Spy technology has advantages over other molecular gluing systems that are available. SpyCatcher and SpyTag, for instance, can glue two proteins together at any point in the protein. "That flexibility allows us many different ways to label proteins and gives us new approaches to assemble proteins together for diagnostic tests," Howarth explained.

Howarth and colleagues are working with Isis Innovation, the University of Oxford's technology transfer company, to find potential partners to bring the Spy system to the market.


'/>"/>

Contact: Michael Bernstein
m_bernstein@acs.org
504-670-4707 (New Orleans Press Center, April 5-10)
202-872-6042

Michael Woods
m_woods@acs.org
504-670-4707 (New Orleans Press Center, April 5-10)
202-872-6293
American Chemical Society


Source:Eurekalert

Related medicine news :

1. Tsai wins innovator award for plan to map molecular path to skin cancer
2. Advances in molecular testing offer new hope for lung cancer patients
3. Scientists identify brains molecular memory switch
4. Study finds molecular signature for rapidly increasing form of esophageal cancer
5. Flip of a single molecular switch makes an old brain young
6. Molecular coordination in evolution: A review in Nature Reviews Genetics
7. Obesity, physical inactivity linked with risk for certain molecular subtype of colorectal cancer
8. UNC-led study documents head and neck cancer molecular tumor subtypes
9. Molecular master switch for pancreatic cancer identified, potential predictor of treatment outcome
10. New details on the molecular machinery of cancer
11. U of M researchers develop a molecular calcium sponge to tackle heart failure
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/26/2017)... ... July 26, 2017 , ... A major challenge many labs face is a ... and a lengthy freeze dry cycle are a few common challenges that can overcome ... well as new accessories and advancements in laboratory freeze dryers that will improve quality ...
(Date:7/26/2017)... St. Petersburg, FL (PRWEB) , ... July 27, 2017 , ... It's time to ... "We loaded the club with great benefits," says Kathy Heshelow, founder of Sublime Naturals and ... a full size therapeutic-grade essential oil each month, mailed by the 5th. , ...
(Date:7/26/2017)... Jupiter, Florida (PRWEB) , ... July 26, 2017 ... ... care for dogs and cats has opened in Jupiter. The state of ... patients (cardiology) and class leading treatments with a goal of providing heart patients ...
(Date:7/26/2017)... ... July 26, 2017 , ... Wendy M. Musielak, a partner at the ... as DuPage County Bar Association’s third Vice President earlier this year. She will serve the ... of the second Vice President, in accordance with the organization’s by-laws. , Musielak ...
(Date:7/25/2017)... SPRINGS, California (PRWEB) , ... July 25, 2017 , ... ... involve cranking up the air conditioner — think homemade gourmet ice cream, sparkling pools, ... inside a gift shop featuring fun mugs, journals, and other must-have knick knacks. Ice ...
Breaking Medicine News(10 mins):
(Date:7/21/2017)... and EDMONTON, Alberta , ... scientists and the University of Alberta in ... data in Nature,s partner journal, Schizophrenia 1 , ... predict instances of schizophrenia with 74% accuracy. This ... severity of specific symptoms in schizophrenia patients with ...
(Date:7/21/2017)... 21, 2017 Did you know that PhRMA member companies ... that combined spending on brand medicines, generics and the supply chain ... accounting for just half of this (7 percent)? Or that ... the world,s venture capital investments in high-growth biopharmaceutical startups? ... ...
(Date:7/17/2017)... -- MedX Holdings, Inc., the manufacturer and global distributor of ... today announced the national roll out of its MedX ... standard for the treatment of low back injuries and ... How it works: MedX ... who prescribe the MedX Home Back Machine Program for ...
Breaking Medicine Technology: