Navigation Links
A classic method for modeling skin cancer is featured in Cold Spring Harbor Protocols
Date:9/4/2007

COLD SPRING HARBOR, N.Y. (Tues., Sept. 4, 2007) Skin cancer is the most prevalent form of human cancer in the United States, according to the Centers for Disease Control. But in order to more fully understand skin cancer in humans, scientists must use model organisms, such as mice, to study the disease in the laboratory.

This months release of Cold Spring Harbor Protocols (www.cshprotocols.org) includes free access to a protocol for generating mice with squamous cell carcinoma (SCC), one of the most common types of skin cancer. The procedure involves injecting mice with a drug called DMBA, which mutates (and thereby activates) a tumor-promoting gene. A second drug, called TPA, then encourages the proliferation of cells that carry the mutated gene. The resulting mass of cells is a tumor.

The protocol, freely available at http://www.cshprotocols.org/cgi/content/full/2007/18/pdb.prot4837, describes how to monitor and evaluate the mice for clinical signs of tumorigenesis. It also includes methods for preparing the tumor tissues for histological analysis, which allows scientists to study characteristics of the tumors at a microscopic level.

The protocol is from Dr. Michael Girardis group at the Yale University School of Medicine (http://info.med.yale.edu/dermatology/dept/girardi.html). Girardis team has used the procedure to examine the role of the immune system in susceptibility to SCC. It can also be used to test other physiological and environmental factors that may influence the growth and progression of skin cancer in mice, and will ultimately help scientists to better understand and control the disease in humans.

Also freely available from Cold Spring Harbor Protocols this month is an article that describes an efficient method for testing individuals for specific DNA variations called SNPs (single-nucleotide polymorphisms) (http://www.cshprotocols.org/cgi/content/full/2007/18/pdb.prot4843). The method, called the oligonucleotide ligation assay (OLA), will be useful for identifying individuals with disease-related mutations and other genetic variants. It was contributed by Dr. Stuart Macdonald from the University of Kansas (http://web.ku.edu/sjmac/).


'/>"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
Source:Eurekalert

Related medicine news :

1. Ultrasound may mean end to classic stethoscope
2. New methods to prevent injury of patients
3. A new method to help smokers quit
4. Methods to have a flat stomach
5. Bioengineering production strains - Microbia develops new methods
6. New method of measuring arterial stiffness in elderly hypertensives
7. Easier Methods Required to Screen Insulin Resistance
8. Easier Methods Required to Screen Insulin Resistance
9. Accuracy of Some Natural Family Planning Methods Questioned .
10. New Method Of Delivering Chemotherapy
11. Adopting Easier Methods To Screen Insulin Resistance
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/26/2017)... ... 26, 2017 , ... On May 24, the Congressional Budget Office (CBO) projected ... on May 4, would result in 23 million Americans losing their health insurance by ... Patient Protection and Affordable Care Act (ACA). , “It is clear from the ...
(Date:5/26/2017)... ... May 26, 2017 , ... Silver Birch of Hammond, a new assisted ... more than four acres of land at 5620 Sohl Avenue in Hammond, serves older ... building includes 125 studio and one-bedroom apartments. Each of the private apartments at Silver ...
(Date:5/26/2017)... ... May 26, 2017 , ... “When the Stars Lead ... is the creation of published author Laura Weigel Douglas, an avid reader who lives ... a house that sometimes feels like Green Hills Adventure Camp. She couldn’t be more ...
(Date:5/26/2017)... ... ... “Just What Happened in the Garden of Eden”: retells the stories of three Bible figures ... of published author, Penelope Colt, mother, trader, horse farmer, artist and a former GM journeyman. ... three. At six, they moved to Dayton, Ohio, where Penny graduated high school. ...
(Date:5/24/2017)... ... 2017 , ... Last month, representatives from Rendina Healthcare Real ... officials to celebrate the grand opening of the 87,000 square foot medical office/ICU ... its ongoing relationship with RWJBarnabas Health, New Jersey’s largest health system. Two years ...
Breaking Medicine News(10 mins):
(Date:5/8/2017)... Mich. , May 8, 2017  Diplomat Pharmacy, ... WRB Communications, Inc. ("WRB"), a health care service ... . WRB specializes in relationship management programs ... WRB will join ... Diplomat,s commercialization support services for manufacturers, biotech firms, and ...
(Date:5/6/2017)... N.Y. , May 5, 2017  Hill-Rom Holdings, ... will add approximately 100,000 square feet to its Welch ... September 2016 its commitment to bring more than 100 ... , where Welch Allyn has maintained a significant presence ... help accommodate these new positions, a large portion of ...
(Date:5/4/2017)... DIEGO , May 4, 2017 ... demonstrating its wireless, handheld ultrasound scanners this week ... Annual Scientific Meeting (ACOG) in San ... "Clarius is the perfect tool ... growth and heart rate, and evaluate pregnancy-related complications ...
Breaking Medicine Technology: