Navigation Links
A better way to count molecules discovered
Date:11/21/2011

Researchers at the Swedish medical university Karolinska Institutet have developed a new method for counting molecules. Quantifying the amounts of different kinds of RNA and DNA molecules is a fundamental task in molecular biology as these molecules store and transfer the genetic information in cells. Thus, improved measurement techniques are crucial for understanding both normal and cancer cells.

It is very difficult to detect small individual molecules in a complex mixture. Therefore, the signal is usually first amplified by making many copies of each molecule. Unfortunately, the copying complicates tracking the exact number of original molecules. The reason is that it is virtually impossible to tell afterwards exactly how many times each original molecule was copied as all copies originating from same type of molecules are indistinguishable from each other.

In an article published by the scientific journal Nature Methods the researchers present a method in which the molecules are first artificially made different in such a way that the copies made from different original molecules can be later distinguished. Then the molecules can be efficiently counted using the new high-throughput sequencers that can read millions of short DNA stretches in parallel. The idea behind the method is astonishingly simple, yet it enables counting the absolute number of molecules in a cell sample whereas many current methods can only measure relative differences between samples.

Professor Jussi Taipale's group applied the new method to simultaneously count thousands of different types of messenger RNA molecules present in cells. The new method proved to be more accurate than the one that has been commonly used for this task. Efficient and reliable counting of messenger RNA molecules is important because their abundances reveal which genes are active in the cells of interest. Professor Taipale's group studies regulation of cell growth and thus wants to understand not only which genes are active in normal cells but also genes that are aberrantly activated in cancer cells.

The new molecule counting method was developed as collaboration between Jussi Taipale's and Sten Linnarsson's groups at Karolinska Institutet, The method has turned out to be especially suitable for counting molecules from a small number of cells. Thus, Sten Linnarsson plans to apply it to counting molecules from a single cell a very exciting and challenging task. The principle of the new method can also be used to improve other important measurement techniques, and to develop technologies that allow more accurate sequencing of genomes of cancer cells and various organisms.

Teemu Kivioja and Anna Vhrautio, the first two authors, are affiliated with both University of Helsinki and Karolinska Institutet.


'/>"/>

Contact: Press Office
pressinfo@ki.se
46-852-486-077
Karolinska Institutet
Source:Eurekalert

Related medicine news :

1. Paving the way for better prevention and management of delirium
2. Newer Drug Seems Better at Controlling Lupus Kidney Complication
3. Exercise may be better than stents for PAD patients
4. Blood-based genomic test better than imaging test for ruling out obstructive coronary artery disease
5. Better understanding of neurologic defects improves post-cardiac-arrest discharge
6. Tis better to give than to receive?
7. In a childhood cancer, basic biology offers clues to better treatments
8. New international health survey of sicker adults: Those with a medical home fare better
9. Prostate cancer surgery better at teaching hospitals
10. Study identifies factors linked with better medication response for treatment of juvenile arthritis
11. Newer Not Necessarily Better for Football Helmet Design
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/25/2016)... ... 2016 , ... Conventional wisdom preaches the benefits of moderation, whether it’s a ... the bar too high can result in disappointment, perhaps even self-loathing. However, those who ... , Research from PsychTests.com reveals that behind the tendency to set ...
(Date:6/24/2016)... ... ... was in a crisis. Her son James, eight, was out of control. Prone to extreme ... “When something upset him, he couldn’t control his emotions,” remembers Marcy. “If there was ... other children and say he was going to kill them. If we were driving ...
(Date:6/24/2016)... , ... June 24, 2016 , ... Comfort Keepers® of ... Cancer Society and the Road To Recovery® program to drive cancer patients to and ... other adults to ensure the highest quality of life and ongoing independence. Getting ...
(Date:6/24/2016)... ... June 24, 2016 , ... The Haute Beauty Network, affiliated ... Weintraub as a prominent plastic surgeon and the network’s newest partner. , ... most handsome men, look naturally attractive. Plastic surgery should be invisible.” He stands ...
(Date:6/24/2016)... ... June 24, 2016 , ... Venture Construction Group (VCG) sponsors Luke’s ... June 20th at the Woodmont Country Club at 1201 Rockville Pike, Rockville, Maryland, 20852. ... helping service members that have been wounded in battle and their families. Venture Construction ...
Breaking Medicine News(10 mins):
(Date:6/24/2016)... -- According to a new market research ... Safety Pen Needles), Needle Length (4mm, 5mm, 6mm, 8mm, ... Purchase (Retail, Non-Retail) - Trends & Global Forecasts to ... for the forecast period of 2016 to 2021. This ... 2021 from USD 1.65 Billion in 2016, growing at ...
(Date:6/23/2016)... HOUSTON , June 23, 2016  MedSource ... platform as its e-clinical software solution of choice.  ... the best possible value to their clients by ... nowEDC.  The preferred relationship establishes nowEDC as the ... pricing for MedSource,s full-service clients.  "nowEDC has long ...
(Date:6/23/2016)...  In a startling report released today, National Safety Council ... a comprehensive, proven plan to eliminate prescription opioid overdoses. ... states are tackling the worst drug crisis in recorded U.S. history, ... Kentucky , New Mexico , ... the 28 failing states, three – Michigan , ...
Breaking Medicine Technology: