Navigation Links
A better way to count molecules discovered
Date:11/21/2011

Researchers at the Swedish medical university Karolinska Institutet have developed a new method for counting molecules. Quantifying the amounts of different kinds of RNA and DNA molecules is a fundamental task in molecular biology as these molecules store and transfer the genetic information in cells. Thus, improved measurement techniques are crucial for understanding both normal and cancer cells.

It is very difficult to detect small individual molecules in a complex mixture. Therefore, the signal is usually first amplified by making many copies of each molecule. Unfortunately, the copying complicates tracking the exact number of original molecules. The reason is that it is virtually impossible to tell afterwards exactly how many times each original molecule was copied as all copies originating from same type of molecules are indistinguishable from each other.

In an article published by the scientific journal Nature Methods the researchers present a method in which the molecules are first artificially made different in such a way that the copies made from different original molecules can be later distinguished. Then the molecules can be efficiently counted using the new high-throughput sequencers that can read millions of short DNA stretches in parallel. The idea behind the method is astonishingly simple, yet it enables counting the absolute number of molecules in a cell sample whereas many current methods can only measure relative differences between samples.

Professor Jussi Taipale's group applied the new method to simultaneously count thousands of different types of messenger RNA molecules present in cells. The new method proved to be more accurate than the one that has been commonly used for this task. Efficient and reliable counting of messenger RNA molecules is important because their abundances reveal which genes are active in the cells of interest. Professor Taipale's group studies regulation of cell growth and thus wants to understand not only which genes are active in normal cells but also genes that are aberrantly activated in cancer cells.

The new molecule counting method was developed as collaboration between Jussi Taipale's and Sten Linnarsson's groups at Karolinska Institutet, The method has turned out to be especially suitable for counting molecules from a small number of cells. Thus, Sten Linnarsson plans to apply it to counting molecules from a single cell a very exciting and challenging task. The principle of the new method can also be used to improve other important measurement techniques, and to develop technologies that allow more accurate sequencing of genomes of cancer cells and various organisms.

Teemu Kivioja and Anna Vhrautio, the first two authors, are affiliated with both University of Helsinki and Karolinska Institutet.


'/>"/>

Contact: Press Office
pressinfo@ki.se
46-852-486-077
Karolinska Institutet
Source:Eurekalert

Related medicine news :

1. Paving the way for better prevention and management of delirium
2. Newer Drug Seems Better at Controlling Lupus Kidney Complication
3. Exercise may be better than stents for PAD patients
4. Blood-based genomic test better than imaging test for ruling out obstructive coronary artery disease
5. Better understanding of neurologic defects improves post-cardiac-arrest discharge
6. Tis better to give than to receive?
7. In a childhood cancer, basic biology offers clues to better treatments
8. New international health survey of sicker adults: Those with a medical home fare better
9. Prostate cancer surgery better at teaching hospitals
10. Study identifies factors linked with better medication response for treatment of juvenile arthritis
11. Newer Not Necessarily Better for Football Helmet Design
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/12/2016)... ... February 12, 2016 , ... Itopia, a ... the integration of Clarity Intelligence Platform (CIP) into Cielo®, a discovery, migration and ... intelligence (BI) to their small and medium business (SMB) clients. , ...
(Date:2/12/2016)... ... 12, 2016 , ... Vail knee specialist Robert LaPrade, MD, PhD ... . The list consists of physicians establishing, leading and partnering with ambulatory surgery centers ... An Ambulatory Surgery Center, also known as an ASC, is a modern health care ...
(Date:2/12/2016)... , ... February 12, 2016 , ... For Coast Dental ... Suzan Sweeney, RDH, and dental assistant Terrell Moore shortly before 7 a.m. to volunteer ... of dental professionals, donating their time and skills to help hundreds of uninsured and ...
(Date:2/12/2016)... Malvern, Pa. (PRWEB) , ... February 12, 2016 ... ... help sort through a world of exterior design possibilities. CertainTeed, North America’s leading ... visualization tools by expanding the product offerings showcased in the mobile version of ...
(Date:2/12/2016)... (PRWEB) , ... February 12, 2016 , ... ... creating explosive growth in the field of long term care. With that, says ... for well-trained healthcare professionals in administrative roles in long term care environments. His ...
Breaking Medicine News(10 mins):
(Date:2/12/2016)... --> ... Global Clinical Trials Review, H2, 2015 provides an ... trials landscape along with top level data related ... & E7), Trial Status, Trial Phase, Sponsor Type ... involved and enlists all trials (Trial title, Phase, ...
(Date:2/11/2016)... 11, 2016 Stem cells are primitive cells ... self-renewal and the capacity to differentiate into mature cell ... as the first mouse embryonic stem cells were derived ... 1995 that the first culturing of embryonic stem cells ... not produced until 2006 As a result of these ...
(Date:2/11/2016)... -- Kindred Biosciences, Inc. (NASDAQ: KIN ), a biopharmaceutical ... pets, today announced the submission to FDA of the ... (NADA) for Zimeta™ (dipyrone injection, KIND-012).  Positive topline results ... the control of pyrexia (fever) in horses were recently ... --> The Chemistry, Manufacturing, and Controls technical ...
Breaking Medicine Technology: