Navigation Links
t-HUVEC

Multiporator Transfection Protocol Protocol No. 4308 915.049 11/2001 Cell line t-HUVEC, transformed human umbilical endothelial cells, EC304 (ATCC) Transfection with pcDNA3-EGFP (endotoxin-free DNA-preparation; in bidistilled H2O) Electroporation buffer Eppendorf Electroporation Buffer with 200 mOsmol/kg Culture medium RPMI 1640 + 10% FCS Cuvette Eppendorf, 2 mm gap width, 400 l Temperature RT (20-25 C) Reference Ina Radtke, Edith Jasny Ruhr-Universitt Bochum Fakultt fr Chemie
Molekulare Neurobiochemie Universittsstrae 150 D-44780 Bochum
Phone +49 234 32 22067 Fax +49 234 32 14105 e-mail ina.radtke@ruhr-uni-bochum.de
  1. Harvest the cells in the exponential growth phase and centrifuge them (for 5 minutes, 160 x g, at room temperature).
  2. Resuspend the cells in RPMI 1640 with 1% FCS, determine the number of cells and centrifuge them (for 5 minutes, 160 x g, at room temperature). Remove supernatant.

    Note: The overall incubation time in the Eppendorf Electroporation Buffer must not exceed 30 minutes to guarantee a successful electroporation!

  3. <
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Saccharomyces cerevisiae
2. Saccharomyces cerevisiae
3. Saccharomyces cerevisiae
4. Electrophoretic Karyotypes of Wine Strains of Saccharomyces cerevisiae
5. Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Varian Cary Eclipse
6. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: HUVEC

(Date:9/22/2014)... Synageva BioPharma Corp. ("Synageva") ... developing therapeutic products for rare disorders announced today ... Chief Operating Officer.  Reporting directly to Sanj ... Robert will oversee the planned global commercial launch ... LAL Deficiency, and will have responsibility for the ...
(Date:9/21/2014)... to produce ultra-thin "diamond nanothreads" that promise extraordinary properties, ... strongest nanotubes and polymers. A paper describing this discovery ... a professor of chemistry at Penn State University, will ... the journal Nature Materials . , "From a ... the threads we formed have a structure that has ...
(Date:9/20/2014)... (PRWEB) September 20, 2014 ... urological and interventional radiology products and services, has ... subsidiary with Clinicon Corporation for its ... HealthTronics’ portfolio of minimally invasive, mobile, surgical technology—offering ... , Under the agreement, HealthTronics will make available ...
(Date:9/19/2014)... disease risks, the biological characteristics people care most about ... heightthe textbook example of a complex traitattributes like risk ... genetic and environmental influences, making it challenging to find ... typically mate two individuals that differ in key waysfor ... study their descendents, looking for genes that tend to ...
Breaking Biology Technology:Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 2Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 3Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 4Smallest possible diamonds form ultra-thin nanothreads 2Smallest possible diamonds form ultra-thin nanothreads 3Smallest possible diamonds form ultra-thin nanothreads 4HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 2HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 3Want to link genes to complex traits? Start with more diversity 2
... National Institutes of Health have turned simple baker,s yeast into ... for drugs to treat Parkinson,s disease. In a study ... researchers showed that they can rescue yeast cells from toxic ... the cells to make very small proteins called cyclic peptides. ...
... , WILMINGTON, Del., July 13 ParagonRx, a ... medications, has launched a new website - www.paragonrx.com ... companies needing information and assistance in complying with new FDA requirements ... and dangerous side effects. , , ...
... , , LEXINGTON, Mass. and HEIDELBERG, ... announced today the launch of the first human cancer biochip for ... Sequencing (NGS). The new catalog cancer biochip features 115 important genes ... by the Wellcome Trust Sanger Institute. , , ...
Cached Biology Technology:Novel drug discovery tool could identify promising new therapies for Parkinson's disease 2Novel drug discovery tool could identify promising new therapies for Parkinson's disease 3Novel drug discovery tool could identify promising new therapies for Parkinson's disease 4ParagonRx Creates New Website Featuring REMS Help Center for Pharma 2First Catalog Cancer Biochip for Sequence Capture Now Available from febit 2
(Date:9/21/2014)... of California, San Diego, have developed a program that ... activity of genes based on sequences of DNA. They ... to human embryonic cells in a paper published in ... of our cells have the same blueprint, the same ... lead author of the report. "Skin cells protect, nerve ...
(Date:9/19/2014)... the Jimmy Durante of dinosaurs a newly ... The new dinosaur, named Rhinorex condrupus ... Brigham Young University, lived in what is now ... Late Cretaceous period. , Rhinorex, which translates roughly ... close relative of other Cretaceous hadrosaurs like Parasaurolophus ...
(Date:9/19/2014)... a species which over thousands of years has adapted to ... substance that makes the cold dangerous for you. , This ... , and the cold provoking substance, called nonylphenol, comes from ... is suspected of being a endocrine disruptor, but when entering ... worm,s ability to protect the cells in its body from ...
Breaking Biology News(10 mins):Program predicts placement of chemical tags that control gene activity 2New hadrosaur noses into spotlight 2Environmental pollutants make worms susceptible to cold 2Environmental pollutants make worms susceptible to cold 3
... belonging to the genus Daphniola was found by a ... spring covered by snow on Mt. Parnassos, central Greece. This study ... . The new species, Daphniola eptalophos , ... a black penis with a small colorless outgrowth on the left ...
... Acinetobacter baumannii (MDR-AB) was found in the ... colonized or infected with the pathogen, according to a ... American Journal of Infection Control , the official ... Infection Control and Epidemiology. The study examined ...
... of Southern California (USC) professor Mary Ann Pentz, Ph.D., shows ... are more likely to drink alcohol in high school than ... September-October 2011 issue of the journal Alcohol and Alcoholism ... that may benefit from special prevention programs. "As you ...
Cached Biology News:Acinetobacter baumannii found growing in nearly half of infected patient rooms 2Underage drinking among close friends high indicator of future alcohol use by black teens 2
Unit Definition: 1 U corresponds to the amount of enzyme which produces 1 mol protons per minute at pH 5.5 and 30C (peptin from citrus peel, Fluka-No. 76280, as substrate)...
ANTI STENOTROPHOMONAS MALTOPHILIA...
... tube handling and reagent dispensing Holds: ... - 96 x 0.3ml U-bottom Omnistrips ... Non-Skirted Plate - Thermo-Fast® 96 Semi-Skirted Plate ... Multiple workstations can be securely stacked Can be stored ...
... well Magnetic-Ring Stand is designed for paramagnetic ... microplates. The stand has a plastic base ... plate footprint. The use of 96 extremely ... easy magnetic separation. With this magnetic stand, ...
Biology Products: