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siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

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4. Kumar, R. Conklin DS, Mittal V. High-throughput selection of effective RNAi probes for gene silencing. Genome Res. 13 (10), 2333–2340 (2003).

5. Luo, B. et al. Small interfering RNA production by enzymatic engineering of DNA (SPEED). Proc. Natl. Acad. Sci. USA 101 (15), 5494–5499 (2004).

6. Berns, K. et al. A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428 (6981), 431–437 (2004).

7. Paddison, P.J. et al. A resource for large-scale RNA-interference-based screens in mammals. Nature 428 (6981), 427–431 (2004).

8. Mousses, S. et al. RNAi microarray analysis in cultured mammalian cells. Genome Res. 13 (10), 2341–2347 (2003).

9. Ramm, P. and Thomas, N. Image-based screening of signal transduction assays. Sci. STKE. 2003 (177), PE14.

10. Price, J.H. et al. Advances in molecular labeling, high-throughput imaging and machine intelligence portend powerful functional cellular biochemistry tools. J. Cell. Biochem. Suppl. 39, 194–210 (2002).

11. Thomas, N. Lighting the circle of life: fluorescent sensors for covert surveillance of the cell cycle. Cell Cycle 2 (6), 545–549 (2003).

12. Thomas, N. et al. Characterization and gene expression profiling of a stable cell line expressing a cell cycle GFP sensor. Cell Cycle 4 (1), (2005).

13. Gu, J. et al. Cell cycle-dependent regulation of a human DN
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