Navigation Links
siRNA screening of the cell cycle with two dynamic GFP sensors (from Discovery Matters Issue 1, July 2005)

M. Kenrick, S. Hancock, S. Stubbs, and N. Thomas
GE Healthcare, The Maynard Centre, Cardiff, UK


Recent advances in siRNA methodologies and the development of high-throughput image analysis platforms such as the IN Cell Analyzer have revolutionized the functional analysis of genes and proteins. Here, we describe the application of two stable cell lines expressing green fluorescent protein (GFP)cell cycle sensors to screen a library of siRNAs directed against key cell cycle control genes. Imaging of GFP intensity and distribution within these two cell lines allows cell cycle position to be assigned by automated image analysis procedures and permits their use in the screening of drugs that block the cell cycle.


Introduction
Recent developments in RNA interference (RNAi) and small interfering RNA (siRNA) techniques for specifically modulating gene expression in a diverse range of cells and organisms (1, 2, 3) have revolutionized the functional analysis of genes and proteins. Advances in synthetic and virally encoded siRNA methodologies (4, 5) have now reached a stage where large scale RNAi screens can be applied to mammalian cells (6, 7, 8). In addition to the provision of large numbers of validated siRNAs, efficient mammalian siRNA functional screens will require information-rich model systems that allow abstraction of multi-parameter data at a level of throughput compatible with large-scale projects. Fortunately, advances in the capabilities of siRNA have been matched by the development of sophisticated fluorescence imagers and software capable of imaging and analyzing cellular events in live cells at high-throughput (9, 10). Such instrumentation enables study of complex systems by combining data from fluorescent cellular sensors with morphological parameters to provide a detailed description of the phenotypic effects of siRNAs in cellular scr
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
(Date:2/27/2015)... 2015 FamilyFarms Group is pleased to ... State Line Farms, received the 2015 Tomorrow’s Top Producer ... the age of 35, who has demonstrated excellence in ... to win the Tomorrow's Top Producer Horizon Award. Every ... Top Producer Award and learning from their experiences. It ...
(Date:2/26/2015)... , Feb. 26, 2015 On behalf ... its affiliated companies, Wilentz, Goldman & Spitzer, P.A. ... secrets lawsuit against Genewiz Inc. in excess of $10 ... utilized GenScript,s trade secrets, and that it improperly hired ... an order denying motions for judgment notwithstanding the verdict, ...
(Date:2/26/2015)... , Feb. 26, 2015 Protiviti ( www.protiviti.com ... version of the Governance Portal , designed to ... and compliance (GRC) tasks across multiple business units and ... to help customers better align their controls with the ... Commission ) requirements. In response to client feedback, Protiviti ...
(Date:2/26/2015)... 2015 The healthcare landscape is rapidly and radically ... Sullivan will host its 20 th Anniversary Medical ... 8 to 10, at the Hilton San Diego Resort and ... This event is a platform for global medical devices, ... disruptions and how to succeed in an uncertain future. ...
Breaking Biology Technology:Matt Sims Receives the 2015 Tomorrow’s Top Producer Horizon Award 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 2Wilentz Client GenScript Awarded $10 Million in Trade Secrets Case Against Genewiz 3Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 2Protiviti Governance Portal Helps Companies Drive Governance, Risk and Compliance Productivity Across Multiple Business Units 3Embrace the Future of Medical Technologies with Frost & Sullivan 2Embrace the Future of Medical Technologies with Frost & Sullivan 3
... - Automotive supplier Johnson Controls unveiled ... American International Auto Show in Detroit. The additions to ... build on their existing technology, increasing ease of use ... ,Moving into a growing market, Johnson showed off its ...
... corporation has a face, captured by its name identity and ... name in the marketplace. Whether you like it or not, ... Following are the Five Faces of Corporate Image. , ,The ... the best suited for you and why. All images are ...
... of his company's patented racks of computer components Who ... in helping IT administrators unpack cardboard boxes? , ,Strange as ... and his colleagues at Paragon Development Systems (PDS) ... has developed an unusual service that makes it easier for ...
Cached Biology Technology:Five faces of corporate image 2Five faces of corporate image 3PDS discovers golden opportunity In 323 dumpsters worth of trash 2PDS discovers golden opportunity In 323 dumpsters worth of trash 3
(Date:1/22/2015)... ("Company") (BM&FBOVESPA: VLID3 ON), is pleased to inform its shareholders ... Washington,s Department of Licensing, in the United States, awarded ... for driver,s licenses and identification cards to its subsidiary, Valid ... start in January 2015, with enrollment and card production starting ...
(Date:1/22/2015)... , Jan. 13, 2015 Technology Showcase, Hall E ... identity authentication solutions, today announced it will showcase its EyeLock ... developed by the Department of Energy,s Oak Ridge National ... Auto Show . EyeLock,s iris identity authentication technology is being ...
(Date:1/22/2015)... , Jan. 20, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... commerce market, reports on the recent success of the Wocket™ smart wallet ... Wocket smart wallet was named as one of the "11 Hot ... "5 Best Products Launched At CES So Far" by Newseveryday.com and "The ...
Breaking Biology News(10 mins):Valid USA Signs Contract For Washington Driver's License Issuance 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 2EyeLock Debuts First Iris Authentication Technology Embedded in 3D Printed Automobile 3CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 2CES Response for NXT-ID's Wocket Smart Wallet Kicks Off 2015 3
... -- Rice University scientists have unveiled a revolutionary new ... into steam. The new "solar steam" method from Rice,s ... even produce steam from icy cold water. Details ... in ACS Nano . The technology has an ...
... after Hurricane Sandy hit, NJIT Professor Michel Boufadel was awarded ... of the storm on the New Jersey shoreline. The ... a team of eight researchers to the beaches of Raritan ... students is still fanning out over the shoreline and ...
... getting, when they select females for mating, according to a ... Wisconsin-Milwaukee in the US. His work, which is one of ... mating behaviors in a nocturnal anuran (frog or toad), is ... . Animals display a number of courtship behaviors and ...
Cached Biology News:Rice unveils super-efficient solar-energy technology 2Rice unveils super-efficient solar-energy technology 3NJIT civil engineer receives NSF grant to study storm's impact on Jersey Shore 2Vision stimulates courtship calls in the grey tree frog 2
... 100 g of monoclonal antibody reactive against ... of SURF1 are highest in growing cultured cells. ... c oxidase which is thought to participate in ... insertion into this subunit. Mutations of SURF1, particularly ...
Rabbit polyclonal to Aortic Elastin ( Abpromise for all tested applications). entrezGeneID: 2006 SwissProtID: P15502...
Anti-gp120/160(SIV/mac239) Immunogen: gp120(SIV/mac239) protein (Genebank accession #M33262)...
Tropomyosin 4 [Tm4]...
Biology Products: