Navigation Links
siRNA Design Guidelines

="chead">

Ambion's Recommended Procedure for siRNA Hairpin Design

The following recommendations for siRNA hairpin design and cloning strategy are made based on research by Ambion scientists. The first step in designing an appropriate insert is to choose the siRNA target site by following the steps described under "General Design Guidelines" above.

For screening, we typically test four siRNA sequences per target, spacing the siRNA sequences down the length of the gene sequence to reduce the chances of targeting a region of the mRNA that is either highly structured or bound by regulatory proteins. Because constructing and testing four siRNA expression plasmids per target is time-consuming, we find it much easier to screen potential siRNA sequences using PCR-derived siRNA expression cassettes (SECs). SECs are PCR products that include promoter and terminator sequences flanking a hairpin siRNA template and can be prepared with Ambion's Silencer Express Kits. This screening strategy also permits the rapid identification of the best combination of promoter and siRNA sequence in the experimental system. SECs found to effectively elicit gene silencing can be readily cloned into a vector for long term studies. Ambion scientists have determined that sequences that function well as transfected siRNAs also function well as siRNAs that are expressed in vivo. The only exception is that siRNA sequences to be expressed in vivo should not contain a run of 4 or 5 A's or T's, as these can act as termination sites for Polymerase III.

For tradition
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. Silencer Validated siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: siRNA Design Guidelines

(Date:9/23/2014)... Albany, New York (PRWEB) September 23, 2014 ... advocates from around the globe will convene ... 11th International Conference, to be held October ... latest in evidence-based research and practice in ... includes physicians, scientists, researchers, nurses, psychologists, nutritionists, ...
(Date:9/23/2014)... CAMBRIDGE, England , September 23, 2014 /PRNewswire/ ... UK launched with funding from Imperial Innovations, Cambridge Innovation ... Inivata, a clinical cancer genomics company focused ... (ctDNA) analysis to improve cancer testing and treatment, ... funding round led by Imperial Innovations and including ...
(Date:9/22/2014)... September 22, 2014 Pearl's Premium Ultra ... in drought tolerant grass seed this week with the ... The announcement coincides with the fact that it is ... as many believe. , As of the date ... favorable reviews by industry experts and the media including: ...
(Date:9/22/2014)... 22, 2014 Research and ... of the "Global Biotechnology Reagents Market 2014-2018" ... (Logo: http://photos.prnewswire.com/prnh/20130307/600769) Biotechnology reagents are chemical ... system to detect and examine another substance to ... in laboratories to prepare, collect, and examine specimens ...
Breaking Biology Technology:International Medical Experts to Discuss Latest Integrative Oncology Research and Its Translation to Personalized Clinical Care at 11th International Conference of SIO 2International Medical Experts to Discuss Latest Integrative Oncology Research and Its Translation to Personalized Clinical Care at 11th International Conference of SIO 3Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 2Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 3Inivata Raises £4m to Realise the Potential of ctDNA Analysis to Improve Cancer Treatment Through Simple Blood Tests 4Pearl's Premium Ultra Low Maintenance Lawn Seed Announces A Major Breakthrough In Drought Tolerant Grass Seed 2Global Biotechnology Reagents Market 2014-2018: Key Vendors are Agilent, BD Biosciences, Bio-Rad, F. Hoffmann-La Roche, Sigma-Aldrich and Thermo Fisher 2Global Biotechnology Reagents Market 2014-2018: Key Vendors are Agilent, BD Biosciences, Bio-Rad, F. Hoffmann-La Roche, Sigma-Aldrich and Thermo Fisher 3
... , a provider of single-molecule DNA analysis technology, announced Thursday ... type of brain tumor, known as oligodendroglioma. OpGen, based in ... and professor of genetics and chemistry at the University ... . , ,Optical mapping, invented by Schwartz, will enable researchers ...
... Roberts, former executive vice president of Oracle Corporation ... businesses can streamline operations using new technology and how ... speech was a part of Accelerate Madisons ... making businesses be more competitive and profitable. ...
... first thing to do when draining a swamp is to ... an information swamp. Information not only needs to be better ... as its passed within an organization. IT shops are skilled ... and even the old, back office systems running on mainframes. ...
Cached Biology Technology:OpGen joins UW, Henry Ford Heath in Improving Brain Tumor Treatment 2Former Oracle VP reveals thoughts on benefits of IT 2Draining the Information Swamp 2Draining the Information Swamp 3
(Date:9/22/2014)... September 23, 2014 A new American Cancer ... Nutrition Assistance Program (SNAP), previously known as the ... compared with income eligible non-participants. The authors say ... aimed at enhancing the dietary quality of SNAP ... low-income individuals and households with the resources to ...
(Date:9/22/2014)... HOUSTON (Sept. 22, 2014) As genetic sequencing ... care, there is a critical need to establish appropriate ... and ethical experts have said. A special policy issue ... published online today and edited by experts with ... Baylor College of Medicine gives policy makers the tools ...
(Date:9/22/2014)... that manufacture their own "antifreeze" proteins to survive ... unfortunate side effect, researchers report: The protein-bound ice ... even when temperatures warm. , The finding ... National Academy of Sciences . , "We discovered ... the evolution of antifreeze proteins in Antarctic notothenioid ...
Breaking Biology News(10 mins):Federal food program puts food on the table, but dietary quality could be improved 2Experts provide much-needed policy analysis for clinical integration of next generation sequencing 2Experts provide much-needed policy analysis for clinical integration of next generation sequencing 3Study: Antifreeze proteins in Antarctic fishes prevent freezingand melting 2Study: Antifreeze proteins in Antarctic fishes prevent freezingand melting 3
... cells, which produce electrical signals in response to vibrations within ... or trauma-related hearing loss. One way to do this could ... to grow. Researchers at Emory University School of Medicine ... cochleae of young mice can induce the formation of extra ...
... University Press (BNUP) will co-publish a new book series ... first seven titles of this series were signed by ... Yang, CEO of BNUP, at this year,s London Book ... supported by both Chinese and international funding, in large ...
... , Using the latest satellite tracking technology, conservationists from the ... Government of Mexico have completed a ground-breaking study on a ... team has produced the first published study on the use ... world,s largest ray, which can grow up to 25 feet ...
Cached Biology News:Gene therapy for hearing loss: Potential and limitations 2Springer and the Beijing Normal University Press sign agreement on new book series 2First satellite tag study for manta rays reveals habits and hidden journeys of ocean giants 2