Navigation Links
siRNA Design: It's All in the Algorithm

using 18S rRNA as the loading control. The reduction in target mRNA is calculated by comparing the PCR results for each test siRNA versus a negative control siRNA. Even extremely low concentrations of Cenix pre-designed siRNAs elicit a strong RNAi response.


There are several advantages to using low concentrations of highly active siRNAs; the most important is minimization of off-target effects, as these are known to increase with increasing concentrations of input siRNA [9, 10]. Off-target effects from high concentrations of siRNAs in the cells are likely to result because more of the sense strand becomes bound to the RISC and degrades transcripts with sequence complementary to the sense strand siRNA, or dsRNA binding proteins that stimulate antiviral response pathways are more likely to be activated and induce the expression of antiviral response genes. Another important advantage to using less siRNA for silencing experiments is that transfecting lower concentrations of siRNAs allows multiple siRNAs to be transfected at the same time. Ambion researchers have simultaneously reduced the expression of as many as five genes using highly active siRNAs designed with the Cenix algorithm (Figure 4).

Figure 4. Multigene Knockdown with siRNAs. Equal amounts of five different siRNAs were mixed and the indicated concentrations of siRNA were transfected into HeLa cells. 48 hours post-transfection, RNA from the cells was recovered and analyzed for target mRNA reduction by real-time PCR, using 18S rRNA as the loading control. The reduction in target mRNA is calculated by comparing the PCR results for each test siRNA versus a negat
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: siRNA Design All the Algorithm

(Date:8/20/2014)... Aug. 20, 2014 /PRNewswire-iReach/ -- A case study ... between the school,s bioengineering department and the Intel® ... Intel® Software Academic Program, UCSD,s research focuses on ... human body.  Photo - ... the work of Dr. Todd P. Coleman ...
(Date:8/19/2014)... 19, 2014 Shimadzu Scientific Instruments ... Prominence-i and Nexera-i, adding to the company’s extensive ... functionality, an intuitive operating environment, and full automation, ... efficient workflow for conventional to ultra-high-speed analysis. ... and intelligent design so users can begin building ...
(Date:8/19/2014)... Robin Williams’ passing is a sad ... an individual. Symptoms range from slowness of voluntary ... and severe depression. Parkinson’s disease progressively gets worse ... producing neurons of the brain. As these patients ... and progressively gets worse, they look for options. ...
(Date:8/19/2014)... 2014   Synthetic Biologics, Inc. (NYSE ... biologic and drug programs targeting specific pathogens that ... its novel C. difficile development program ... Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC), ... Synthetic Biologics, Senior Vice President, ...
Breaking Biology Technology:Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 3As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 2As We Mourn Robin Williams’ Passing, His Death Sheds Light on Patients Struggling with Parkinson’s Disease 3Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 2Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 3Synthetic Biologics Announces Late-Breaking Poster Presentation for C. difficile Program at 54th ICAAC 4
... Kathleen A. Maguire-Zeiss, Brandon K. Harv e y, Rita E. Giuliano and,H ... , Introduction , ... number of factors, including , method ... transfection of mammalian cells has proven to yield ...
... Menu B. Leddy, Biotechnology Research Department,Orange County ... , Introduction ... of bacterial species from membrane , ... of bacterial cells from membrane biofilms cannot be ...
... , Introduction , ... use as primers , or probes in genetic ... traditionally been done by slab gel electrophoresis or HPLC, but these , ... not provide desired quantitative precision or automation. Capillary ...
Cached Biology Technology:Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities 2Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities 3Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities 4Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities 5Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities 6Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A 2Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A 3Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A 4Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A 5Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A 6Separation of 16S rRNA PCR Products From a Model Community Using Temporal Temperature Gradient Gel Electrophoresis, Rev A 7Oligonucleotide Purity Analysis by Capillary Electrophoresis 2Oligonucleotide Purity Analysis by Capillary Electrophoresis 3
(Date:8/20/2014)... screening outcomes of approximately 3 million infants, a team ... the University of Massachusetts Medical School, have shown that ... successfully implemented across public health newborn screening programs. Data ... 20 issue of the Journal of the American ... of SCID in newborns is higher than previously thought ...
(Date:8/20/2014)... that acral melanomas the rare type of skin cancer ... distinct from other more common types of skin cancer, according ... Pigment Cell & Melanoma Research . , Acral melanoma ... the feet, nail-beds and other hairless parts of the skin. ... by UV damage from the sun. , The team, from ...
(Date:8/20/2014)... into a chili pepper causes a burning spiciness that ... exploring the chili pepper,s effect are using their findings ... of pain, which can be caused by inflammation or ... which is being tested in clinical trials, in ACS, ... and colleagues explain that decades ago, scientists had pegged ...
Breaking Biology News(10 mins):Newborn screening expansion offers early diagnosis and treatment to infants with SCID 2Newborn screening expansion offers early diagnosis and treatment to infants with SCID 3Scientists learn more about rare skin cancer that killed Bob Marley 2
... after the world,s first ultrasonic detection device invented ... Virginia Tech Carilion Research Institute scientists have ... have long suspected: ultrasound applied to the periphery, such ... to the brain. And that,s just the ...
... ship is at anchor for longer periods algae, shells and ... economic losses of billions of Dollar. Biological growth on the ... the hull below the waterline which has a braking effect ... basified bio layer, the consumption of fuel can increase by ...
... a simple "drag-and-drop" computer interface and DNA self-assembly ... drug development that could drastically reduce the time ... work supported by a National Science Foundation (NSF) ... NanoLabs of Reston, Va., recently developed and began ...
Cached Biology News:Neuroscientists prove ultrasound can be tweaked to stimulate different sensations 2Neuroscientists prove ultrasound can be tweaked to stimulate different sensations 3Keeping ship hulls free of marine organisms 2Drag-and-drop DNA 2Drag-and-drop DNA 3Drag-and-drop DNA 4
... This DuoSet ELISA Development kit contains the ... sandwich ELISAs to measure natural and recombinant ... and serum. Each kit contains sufficient materials ... plates, provided that the following conditions are ...
Blimp-1 (H-150)...
Mouse Galectin-1 Affinity Purified Polyclonal Ab...
PSGL-1 (215)...
Biology Products: