Navigation Links
siRNA Design: It's All in the Algorithm

ured by qRT-PCR and normalized against 18S rRNA from samples harvested 48 hr after siRNA transfection into HeLa cells.


In subsequent analyses of sets of multiple siRNAs targeting the same transcripts, Cenix noted that certain genes, and in some cases certain transcripts, appear significantly more refractory to siRNA-based silencing than the majority (data not shown). Since it was not known what percent of genes would fall into this category, it became clear that any extrapolations of silencing success rates from tens or even hundreds of siRNAs would be of quite limited statistical value. Thus, expecting that the eventual "true" success rate of its algorithm would likely be lower than the 94% initially observed with the 79 kinases, Cenix extended the same performance analysis to a much larger set of siRNAs to create a more statistically relevant data set. Figure 2 shows the distribution of the siRNA efficacy for over 1,100 siRNAs targeting nearly 400 endogenously-expressed human transcripts.

The comprehensive survey indicated that when three Cenix designed siRNAs per gene were tested, one or more of the siRNAs achieved >70% silencing for over 93% of tested genes, >80% silencing for nearly 80% of tested genes, and >90% silencing for approximately half of tested genes. On a per siRNA basis, approximately 80% of the individual siRNAs showed >70% silencing of their target. The performance data clearly confirm the success of the Ceni
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: siRNA Design All the Algorithm

(Date:5/5/2015)... 05, 2015 Research and Markets ... the "Clinical Laboratory, Molecular Diagnostics and Genomic ... 2019 - Global Version" report to their ... the area of molecular diagnostics and pharmacogenics hold ... world market that is moving out of the ...
(Date:5/5/2015)... May 05, 2015 Exotic ... distributor, announces the launch of http://www.cng.exoticautomation.com , ... with a convenient way to purchase genuine OEM ... . , The new website provides owners ... with direct access to high performance products for ...
(Date:5/4/2015)... 4, 2015 Many of us take ... certain infants with rare, inherited mutations of certain ... of their impaired immune responses. Now, ... an important role for calcium signaling in immune ... the bacterium causing tuberculosis (TB). ...
(Date:5/4/2015)...  Tikcro Technologies Ltd. (OTCQB: TIKRF) announced today that ... for the fiscal year ended December 31, 2014 with ... report on Form 20-F contains information regarding its biotechnology ... of cancer immune checkpoints. This project aims to develop ... binding in targeting certain identified immune checkpoints. The filing ...
Breaking Biology Technology:Global Clinical Laboratory, Molecular Diagnostics and Genomic Testing Services Market Trends and Forecast 2015 to 2019 2Exotic Automation & Supply Launches Ecommerce Website 2Researchers Discover A Novel And Unexpected Role For Calcium In Controlling Inflammation During Chronic Lung Infection 2Researchers Discover A Novel And Unexpected Role For Calcium In Controlling Inflammation During Chronic Lung Infection 3Tikcro Technologies Files Annual Report on Form 20-F for the Year Ended December 31, 2014 2
... exclusive, distribution agreement with AZPA International Inc. for Australia and New ... ... LAVAL, QC, Dec. 19 /PRNewswire-FirstCall/ - Neptune Technologies &,Bioressources Inc. ("Neptune") ... an exclusive distribution agreement with,AZPA International Inc. ("AZPA International") for Australia ...
... collaborators, LineaGen is leveraging new funding round, Utah Population ... platform for commercializing first-in-class molecular diagnostic ... ... Inc.,( http://www.LineaGen.com ), a biomarker discovery company created to,discover and commercialize ...
... the Announcement of a Tracking Program for,Cloned Livestock, ... a,statement by International Dairy Foods Association President and ... a new national registry to track cloned,livestock as ... over food,products from cloned animals. However, we strongly ...
Cached Biology Technology:Nationwide product launch of NKO(R) planned for February 2008 in Australia and New Zealand 2Nationwide product launch of NKO(R) planned for February 2008 in Australia and New Zealand 3LineaGen Inc. Launches With Close of $5.8 Million Series A Funding Round 2LineaGen Inc. Launches With Close of $5.8 Million Series A Funding Round 3LineaGen Inc. Launches With Close of $5.8 Million Series A Funding Round 4Statement of International Dairy Foods Association President and CEO Connie Tipton 2
(Date:4/20/2015)... The announcement comes as demand for fire ... Ireland,s foremost records management company, has ... an impressive track record of clients within the first 12 ... management sector in Dubai . As a ... employ a further eight staff members at its Irish headquarters. ...
(Date:4/14/2015)... YORK , April 14, 2015  HYPR ... IDentity Online (FIDO ® ) Alliance tm , ... specifications. FIDO members commit to share technology and ... methods that are interoperable, more secure and private, ... enable biometric identity verification that protects sensitive user ...
(Date:4/13/2015)... , April 13, 2015 ... TechSci Research, "Global Biometrics Market Forecast & Opportunities, 2020", ... a CAGR of around 14% till 2020. The ... for large scale implementation and review of biometric ... launch of new products with greater efficiency, are ...
Breaking Biology News(10 mins):Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3HYPR Corp. Joins the FIDO Alliance 2HYPR Corp. Joins the FIDO Alliance 3Global Biometrics Market to Cross US$ 21 Billion by 2020: TechSci Research 2
... in Hong Kong this year. So far, 466 children were infected ... Streptococcus pyogenes, a gram-positive pathogen that can be transmitted via ... Hong Kong Center for Disease Control (HK CDC), Hong Kong University ... the S. pyogenes strain that was isolated from a ...
... Va. recently found a way to improve electricity generating ... less expensive. Specifically, they discovered a way to speed ... which are necessary for fuel cells to operate. ... membrane, or PEM, commonly used in fuel cells and ...
... and the USDA Agricultural Research Service have developed a new ... boniato, or tropical sweet potato, Liberty has a dark red ... Boniato potatoes originated in the tropical Americas and are grown ... served fried, mashed or in soup. "We developed Liberty ...
Cached Biology News:Hong Kong University leads the genomics research of scarlet fever pathogen 2Stretching old material yields new results for energy- and environment-related devices 2
Fast, sensitive quantitation of Mouse IgM concentration....
Detect and measure levels of mouse IgG3 antibody isotypes quickly and easily....
Request Info...
ExonBio Service provides one-stop recobinant protein shopping from cloning, expression and purification. Price including gene cloning....
Biology Products: