Navigation Links
siRNA Design: It's All in the Algorithm

aiming to offer high success rates for silencing human genes. The purported performance of some of the resulting siRNA design algorithms has been based on largely statistical extrapolations and/or experimental short-cuts such as silencing of cotransfected exogenous reporter constructs. In collaboration with Ambion, Cenix BioScience has chosen the more rigorous path of directly measuring the effectiveness of approximately 1,100 of its own algorithm-designed siRNAs in silencing almost 400 endogenously expressed human transcripts. Importantly, the analysis was carried out in cultured human cells under stringently standardized experimental conditions. This empirical study therefore represents the most physiologically relevant, direct, and comprehensive performance analysis of any siRNA design algorithm available today.


Maximize Success Rate of siRNAs
Publicly available siRNA design programs have so far shown success rates of 50-60% in generating siRNAs that can yield over 70% silencing of target mRNA levels in HeLa cells after 48 hr. Based on this benchmark, Cenix conducted a first test of its algorithm's success rate by measuring silencing efficacy under the same conditions, namely using quantitative RT-PCR to measure target mRNA levels in HeLa cells 48 hr after transfection, for a set of 79 algorithm-designed siRNAs, each of which targeted a different kinase transcript. The analysis revealed that 74 of the 79 siRNAs, or 94% of the tested siRNAs, gave higher than 70% silencing (see Figure 1).

Figure 1. Silencing Efficacy of 79 siRNAs Designed Using Cenix Algorithm to Target 79 Endogenously-expressed Human Kinases. Target mRNA levels were meas
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: siRNA Design All the Algorithm

(Date:1/22/2015)... (PRWEB) January 22, 2015 Controlled Substance ... Alliance, which helps companies check the legal requirements around ... expand to China. , As their international operations ... globally adopting software solutions built as a result of ...
(Date:1/22/2015)... , Nueva Jersey , 22 de enero de ... Biomedical Research abre hoy su llamada a nominaciones para ... investigación científica ha hecho, o tiene el potencial para ... humana. Las nominaciones se aceptarán hasta el 15 de ...
(Date:1/22/2015)... 22, 2015   GenoSpace , a precision medicine software company ... the broad use of genomic, imaging and other biomedical data ... Michelle Munson , CEO of Aspera, an IBM Company, ... - http://photos.prnewswire.com/prnh/20150122/170713 "We are pleased ...
(Date:12/24/2014)... 2014  United Therapeutics Corporation (NASDAQ: UTHR ) ... ) has submitted a pre-market approval application to the ... of Medtronic,s SynchroMed ® II implantable drug infusion ... United Therapeutics, Remodulin ® (treprostinil) Injection delivered intravenously ...
Breaking Biology Technology:Global Compliance Service for Controlled Substances to Expand to China 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 2El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 3El Dr. Paul Janssen Award for Biomedical Research emite su llamada a los nominados para 2015 4GenoSpace Expands Board with Appointment of Michelle Munson 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 2United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 3United Therapeutics Announces Submission Of Pre-Market Approval Application For Implantable Drug Infusion System To Deliver Remodulin 4
... Safeguard Mezzanine Debt Facility; Support Profitable GrowthALISO VIEJO, Calif., ... (Nasdaq: CLRT ), a premier anatomic pathology ... the pharmaceutical industry, announced today that it has completed ... preferred stock with Oak Investment Partners ...
... May 18 /PRNewswire-FirstCall/ - Oncothyreon Inc. (Nasdaq: ... American Society of Clinical Oncology (ASCO) Annual Meeting being ... related to our product candidates Stimuvax(R), PX-866 and PX-12 ... ASCO Web site, www.abstract.asco.org ., ...
... ATLANTA, May 18 /PRNewswire-Asia/ -- Global biomedical sciences ... the year 2008. This rides on,Singapore,s strong scientific ... in 2007. , The ... investment environment, as companies look to the fast ...
Cached Biology Technology:Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 2Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 3Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 4Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 5Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 6Oncothyreon announces presentations at American Society of Clinical Oncology (ASCO) Annual Meeting 2Oncothyreon announces presentations at American Society of Clinical Oncology (ASCO) Annual Meeting 3Biomedical Sciences Companies Expanding in Asia Invested More Than US$500 Million in Singapore 2Biomedical Sciences Companies Expanding in Asia Invested More Than US$500 Million in Singapore 3Biomedical Sciences Companies Expanding in Asia Invested More Than US$500 Million in Singapore 4Biomedical Sciences Companies Expanding in Asia Invested More Than US$500 Million in Singapore 5
(Date:12/22/2014)... Dec. 22, 2014  NXT-ID, Inc. (NASDAQ: NXTD ... focused on the growing mobile commerce market, announces it has ... for the 2015 International CES debut of the Wocket™ ... will be at the NXT-ID booth January 6th and 7 ...
(Date:12/19/2014)... Markets ( http://www.researchandmarkets.com/research/8kmfcn/iphone_5s ) has announced the ... Apple/AuthenTec TMDR92 & Sapphire - Technology Report" report ... Following the acquisition of AuthenTec in ... the iPhone 5S. It is currently the only device ...
(Date:12/19/2014)... VEGAS , Dec. 18, 2014   LaunchKey , ... for the post-password and Internet of Things era, today ... funding. The venture round was led by Metamorphic Ventures ... Rimrock Venture Partners, VegasTechFund, and others.  LaunchKey has raised ...
Breaking Biology News(10 mins):NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 2NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 3NXT-ID Hires Master Pickpocket and Security Consultant Apollo Robbins for Wocket Smart Wallet CES 2015 Debut 4iPhone 5S Fingerprint Sensor - Apple/AuthenTec TMDR92 & Sapphire - Technology Report 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 2LaunchKey Raises $3 Million in Additional Funding Led by Metamorphic Ventures 3
... to have a bad reputation. They sleep all day, party at ... study by University of Calgary researchers has confirmed that bats are ... that bats have high rates of rabies is not true," says ... the lead author of a paper published in the Journal ...
... Research by the University of Exeter has revealed that ants ... result of their activity as ,ecosystem engineers, and predators. ... , found that ants have two distinct effects on their ... building activity and by collecting food they affect the level ...
... Scripps Research Institute scientists have converted adult skin ... to first go through the laborious process of generating ... lead to new treatments for a range of diseases ... heart disease, Parkinson,s, and Alzheimer,s disease. The work ...
Cached Biology News:Researchers bust bat rabies stereotype 2Ants' ecosystem role is 'key' 2Scripps Research scientists convert skin cells to beating heart cells 2Scripps Research scientists convert skin cells to beating heart cells 3
... FLUOstar OPTIMA is a fully automated ... life science labs in academia and ... measurement principles: Fluorescence Intensity ... Time-Resolved Fluorescence including DELFIATM ...
... save space and organize gloves, ... other garb. They are the ... small parts used in labs, ... Open-slot design for easy cleaning. ...
... Security ChemStation is a ... application for data acquisition, ... searching and reporting, addressing ... data security, integrity, and ...
... microRNA expression profiling and built on ... on-chip synthesis platform. These microarrays are ... microRNA Expression Profiling Service. ... Microarray contains all known mammal microRNAs ...
Biology Products: