| HOME >> BIOLOGY >> TECHNOLOGY |
Chris Echeverri, Christoph Sachse,
Andrew Walsh, Anne Grabner
Cenix BioScience GmbH
David Brown
Ambion, Inc.
RNA interference (RNAi) has become a powerful
tool for understanding gene function. It is a cellular process
wherein short double-stranded RNAs called short interfering
RNAs (siRNAs) direct the degradation of transcripts containing
sequence complementary to at least one of the siRNA strands
[1,2]. siRNAs trigger RNA degradation through a protein
complex referred to as the RNA Induced Silencing Complex
(RISC) [3]. Most evidence indicates that the RISC contains
only one of the two siRNA strands [4], suggesting that there
is a step prior to, or during, the incorporation of the siRNA
into the RISC that eliminates one of the siRNA strands [5].
Either siRNA strand can be taken up by the RISC [5,6], but the
RISC can only direct degradation of cellular RNAs that are
complementary to the bound siRNA. Recent work suggests that
strand selection can be affected by the nucleotide composition
of the siRNA [7,8]. It is therefore possible to select siRNA
target sites that favor incorporation of the antisense siRNA
strand into the RISC to increase the percentage of RISCs
containing the correct targeting siRNA strand. This ultimately
results in improved efficacy and specificity of the siRNA.
siRNA Design Algorithms
Several siRNA design algorithms have
emerged over the last year cl
'"/>
Source:
Related biology technology :
1. Custom and library siRNA for efficient gene silencing
2. Custom and library siRNA for efficient gene silencing
3. Cancer siRNA Oligo Set Version 1.0
4. Library siRNA
5. Custom siRNA Oligo Synthesis Service
6. Efficient RNAi-mediated gene silencing in neuronal cells using QIAGEN siRNA and TransMessenger Transfection Reagent*
7. Quantification of siRNA Silencing Efficiency Using the LightCycler System
8. Housekeeping Genes: Universal Positive Controls in siRNA Knockdown Experiments
9. Confirming gene silencing mechanism by pGFP/GFP22 siRNA co-transfection
10. siRNA transfection optimization with the Agilent 2100 bioanalyzer
11. siRNA Design Guidelines
| Copyright © 2003-2012 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |