( LCM Staining Kit ...)Protect RNA and Improve Target Cell Identification

Superior identification of target cells provided by histological and fluorescent stains

Designed for use with frozen, OCT-embedded tissue sections (6-10 m)

Compatible with Arcturus LCM Systems

Use with Ambion's RNAqueous-Micro RNA Isolation Kit and MessageAmp II aRNA Amplification Kit for superior gene expression analysis

LCM for RNA Analyses

Laser capture microdissection (for overview see Laser Capture Microdissection (LCM): How its Done, at right) is a method that allows scientists to selectively collect pure cell populations from tissue sections. For RNA studies, frozen, OCT-embedded tissue is generally preferred over formalin-fixed, paraffin-embedded tissue--formalin fixation introduces cross-links between nucleic acids and proteins, resulting in RNA fragmentation. However, RNA extraction from frozen tissue is also complicated by reactivation of endogenous RNases that degrade RNA when tissue sections are exposed to aqueous solutions, as is the case with conventional staining protocols.

To overcome the technical problems of obtaining high-quality RNA from LCM samples, Ambion scientists have developed the LCM Staining Kit for frozen, O
'"/>

Source: