Navigation Links
prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

lexity; Figure 2) or 10 pg (high complexity; data not shown) of reverse-transcribed total RNA. Even low-abundance transcripts can be detected, as shown by the successful amplification of 1.8- to 7.6-kb fragments of human dystrophin from reverse-transcribed total RNA (200 ng of input RNA) (Figure 1).

Figure 2

ProSTAR HF Single-Tube RT-PCR System

The ProSTAR HF Single-Tube RT-PCR system includes all reagents necessary to carry out cDNA synthesis and high-fidelity PCR amplification in a convenient one-tube format (MMLV-RT, TaqPlus Precision DNA polymerase, HF RT-PCR buffer, dNTPs, and RNase-free water). Simply combine reagents into one tube along with gene-specific primers; cDNA synthesis and PCR amplification take place successively during an uninterrupted thermal-cycling program.

Figure 3

With this system, accurately amplify a broad range of target sizes can be accurately amplified from a variety of RNA sources (Figure 3). The kits TaqPlus Precision DNA polymerase blend permits high product yields for amplifications of both relatively short targets (300 to 550 bps; Figure 3, Panel A) and long targets from low-abundance message (1.8- to 5.9-kb dystrophin fragments; Figure 3, Panel B, Lanes 2 to 4). To demonstrate the systems sensitivity, a 325-bp RT-PCR product was amplified from as few as 10 molecules of an in vitro transcribed RNA (Figure 4). In addition, as little as 50 pg of total RNA was sufficient for amplifying the 1.8-kb dystrophin fragment from total RNA (low-abundance message; data not shown).

Figure 4

Both the ProSTAR systems also include test
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. Performance Comparisons of Commercial RT-PCR Systems
5. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
6. RT-PCR Primers for the Study of Apoptosis
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: prostar PCR Systems for Robust High Fidelity RNA Amplification

(Date:8/28/2014)... month, executives from clinical trial marketing firm BBK Worldwide will ... Patient-Centered Clinical Trials 2014 , to be held at the ... 4-5. Patient recruitment experts Bonnie A. Brescia , ... insights on the benefits of employing an effective patient engagement ... to mobile apps – can be used to drive study ...
(Date:8/28/2014)... TALLAHASSEE, Fla. Exciting new work by a Florida ... molecular system that can take your temperature, emit white ... , And, the molecule looks like a butterfly. , ... and Biomedical Engineering in the FAMU-FSU College of Engineering, ... ago, but has continued to discover that his creation ...
(Date:8/28/2014)... 28, 2014 PrimeSource Building Products, ... selected SoundConnect to support their communication ... audio and web conferencing platform GlobalMeet powered by ... employees’ needs. SoundConnect delivers award winning “results ... Building Products will be utilizing GlobalMeet powered ...
(Date:8/27/2014)... Green & Grow Inc. (GGI) has raised an ... Otter Capital as a significant new partner. Otter Capital’s ... Agriplier™ technology, building on recent compelling field trial results ... meeting, we have been impressed with Otter Capital’s knowledge ... Sobba, President and CEO of GGI. “We look forward ...
Breaking Biology Technology:BBK Worldwide Leads Sessions at Key September Events 2Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices 2PrimeSource Chooses SoundConnect as Collaboration Provider 2Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 2Green & Grow Inc. Secures $6M Series B Funding from Otter Capital 3
... Research Society awarded Walter A. de Heer, professor of physics ... its annual fall meeting in Boston today. De Heer was ... science and technology of epitaxial graphene." The MRS Medal recognizes ... years. The MRS Medal is awarded for a specific outstanding ...
... Physicians at Thomas Jefferson University Hospital reported today ... fully automated interpretation of coronary CT angiography (cCTA) by ... artery disease (CAD) in the major coronary arteries as ... Chicago at the Radiological Society of North America (RSNA) ...
... tiny, hair-like blood vessels in the back of our ... vessels like those that bring blood to the heart respond ... revealed that otherwise healthy people exposed to high levels of ... higher risk of heart disease. Previous studies linked pollution ...
Cached Biology Technology:Georgia Tech's Walt de Heer awarded Materials Research Society Medal 2Rcadia COR Analyzer System Safely Rules Out Coronary Artery Disease in Main Coronary Arteries and Branch Vessels 2Photos of tiny blood vessels in the eye link air pollution to heart disease 2
(Date:8/28/2014)... systems are good examples of complex systems. Their ... networkincluding water for cooling, transport to supply fuel, ... step in the network chain is interconnected with ... dependent. A team of UK-based scientists has studied ... The findings have been published in EPJ B ...
(Date:8/28/2014)... More than 25 million kilometres of new roads will ... will slice into Earth,s last wildernesses, where they bring ... , Now, an ambitious study has created a ... to try to balance the competing demands of development ... an ,environmental-values, layer that estimates that natural importance of ...
(Date:8/28/2014)... to alert the world to Global Warming, has called ... stored underground. He says that Carbon Capture, combined with ... to avoid global warming getting out of control over ... York) made the call during his presentation to the ... are meeting to discuss Carbon Capture and Storage. ...
Breaking Biology News(10 mins):Inter-dependent networks stress test 2Study shows where on the planet new roads should and should not go 2Study shows where on the planet new roads should and should not go 3Global warming pioneer calls for CO2 to be taken from atmosphere and stored underground 2
... TALLAHASSEE, Fla. -- One of the world,s most ... brain evolution of modern humans and their ancestors, according ... The Taung fossil the first australopithecine ever discovered ... Falk and a group of anthropological researchers. Their findings, ...
... Sustainability has become a major focus for federal, ... This National Research Council symposium will showcase ... identify opportunities to help promote practices that would ... will highlight science and technology research contributions to ...
... from the European Alps suggests that population declines to ... the long-term effects of recent climate warming on mountain ... from the Department of Conservation Biology, Vegetation and Landscape ... modeling tool to predict migration of mountain plants which ...
Cached Biology News:Anthropologist finds explanation for hominin brain evolution in famous fossil 2European mountain plant population shows delayed response to climate change 2
Gridded Cell Culture Petri Dish (20 mm molded in base), tissue-culture treated...
Chicken Anti-Human DNA-directed RNA Polymerase II 7.6 Kd Polypeptide (POLR2L Polyclonal AntibodyOther Sub-Family: not assigned-Other Applications: ELISA, Western Blot...
... RPMI 1640 with 2 mM L-glutamine and harvested ... order to keep the antigens in their native ... cells are arrayed on a 12-well (5 mm) ... treated to enhance cellular attachment and to minimize ...
...
Biology Products: