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prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification

cDNA template during the PCR portion of RT-PCR procedures.

Table 1
RT-PCR Fidelity

cDNA Amplification Fidelitya

DNA Polymerase

Error Rate
(x 10-6)b

Blue Plaques
(Number of mutants)

Total
Plaques

Mutation Frequency (%)

Pfu

1.3

122

10,441

1.2

TaqPlus Long

7.6

411

12,086

3.4

Taq

8.0

653

12,008

5.6

The PCR fidelity assay was carried out7,10 except that the lacIOlacZa target was amplified from the same cDNA synthesis reaction mixture using different PCR enzymes. For cDNA synthesis, MMLV-RT was used essentially as described in the ProSTAR Ultra HF RT-PCR system protocol using in vitro transcribed RNA.

bmutation frequency/bp/duplication; error rates measured on DNA templates.3,6

To demonstrate that DNA polymer
'"/>

Source:


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