Navigation Links
prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification


Powerful high-fidelity RT-PCR systems in two convenient formats

Michael Borns Janice Cline Kris Connors Ronda Allen Holly Hogrefe
Stratagene

Stratagenes prostar RT-PCR systems are powerful systems that permit efficient, high-fidelity amplification of RNA in two convenient formats. The ProSTAR Ultra HF RT-PCR system features pfuturbo DNA polymerase * for amplifying cDNAs with the highest replication fidelity possible using a versatile two-tube format. The ProSTAR HF Single-Tube RT-PCR system combines the sensitivity and accuracy of the TaqPlus Precision DNA polymerase ** blend with the convenience of a one-step, one-tube procedure. These two systems are ideally suited for subcloning, expression, and sequencing of DNA coding regions and for reproducibly detecting and analyzing RNA molecules.

Traditional techniques used to study gene expression include Northern blotting, RNase protection, in situ hybridization, dot blots, and S1 nuclease assays. However, because coupled reverse transcription and PCR amplification (RT-PCR) is an easy, sensitive, and versatile technique, it is the method of choice for analyzing RNA. RT-PCR can be used to determine the presence or absence of a transcript, to quantify expression levels, and to clone rare messages without needing to construct and screen cDNA libraries.

RT-PCR kits differ not only with respect to the RT and PCR enzymes employed, but also with the procedures whereby cDNA synthesis and PCR amplification are coupled. MMLV reverse transcriptase (MMLV-RT), AMV RT, and Tth DNA polymerase are most commonly used to perform cDNA synthesis.1,2,3,4 Second-strand synthesis and DNA amplification can then be carried out using Taq or Tth DNA polymerase,1,2,3,4 or more recently, Taq-based DNA polymerase blends. Applying these ble
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. Performance Comparisons of Commercial RT-PCR Systems
5. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
6. RT-PCR Primers for the Study of Apoptosis
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: prostar PCR Systems for Robust High Fidelity RNA Amplification

(Date:8/28/2014)... , Aug. 28, 2014  Next month, executives from clinical ... key industry events beginning with Patient-Centered Clinical Trials 2014 ... Boston , September 4-5. Patient recruitment experts ... Aaron Fleishman will share insights on the benefits ... variety of tactics – from media to mobile apps – ...
(Date:8/28/2014)... 2014 Sterlitech is proud to announce ... its stable of products . These additional ... and include membrane filters with surface charges. ... demand for our membrane process testing equipment, and correspondingly, ... sheet membranes,” explains Sterlitech President Mark Spatz. “The ...
(Date:8/28/2014)... Exciting new work by a Florida State University research ... can take your temperature, emit white light, and convert ... molecule looks like a butterfly. , Biwu Ma, associate ... in the FAMU-FSU College of Engineering, created the molecule ... continued to discover that his creation has many other ...
(Date:8/28/2014)... Woodstock, Vt. (PRWEB) August 28, 2014 ... microbiology testing around the world is available from ... industrial diagnostics companies. “ Industrial Microbiology Market Review, ... the Industrial Market ” (IMMR—4) tracks and compares ... in in North America, Europe and Asia, and ...
Breaking Biology Technology:BBK Worldwide Leads Sessions at Key September Events 2Sterlitech Corporation Enhances Its Selection of Flat Sheet Membrane Filters 2Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 22 Billion Industrial Microbiology Tests Conducted for Product Quality and Safety Worldwide 3
... Beckman Coulter,s Worldwide Position in Clinical Chemistry- ... Chemistry and Automation Broaden Global Opportunity, Increase ... of Beckman Coulter,s Immunoassay Products into Olympus, ... Transaction Expected to be Accretive to Non-GAAP ...
... Martin (NYSE: LMT ) has partnered with ... energy sector. As part of the corporation,s commitment to tackling ... research and development projects at Penn State in ... Lockheed Martin - Penn State collaboration will focus ...
... Pressure BioSciences, Inc. (Nasdaq: PBIO ) ... EdgeWater Research Partners, Micro/Small-Cap Investor Conference to be held ... on Wednesday, March 4, 2009. Mr. Richard T. ... PBI Chairman, will give a corporate overview at 10:35 ...
Cached Biology Technology:Beckman Coulter to Acquire Lab-Based Diagnostics Business From Olympus Corporation 2Beckman Coulter to Acquire Lab-Based Diagnostics Business From Olympus Corporation 3Beckman Coulter to Acquire Lab-Based Diagnostics Business From Olympus Corporation 4Beckman Coulter to Acquire Lab-Based Diagnostics Business From Olympus Corporation 5Beckman Coulter to Acquire Lab-Based Diagnostics Business From Olympus Corporation 6Lockheed Martin Invests in Smart Grid Research and Development at Penn State University 2Pressure BioSciences, Inc. to Present at the EdgeWater Research Partners' Spring 2009 Micro/Small-Cap Investor Conference 2
(Date:8/31/2014)... discovered that ,good, cholesterol is turned ,bad, by a ... was found to damage ,good, HDL cholesterol, which removes ... , Low levels of HDL, High Density Lipoprotein, are ... MG being common in the elderly and those with ... from the British Heart Foundation (BHF) and published in ...
(Date:8/31/2014)... from the last 19 years reveals that fresh water from ... Antarctica to rise by 2cm more than the global average ... the rapid rise in sea-level by studying satellite scans of ... , The melting of the Antarctic ice sheet ... excess of around 350 gigatonnes of freshwater to the surrounding ...
(Date:8/29/2014)... at the Barshop Institute for Longevity and Aging Studies, ... Health Science Center at San Antonio, have found another ... rodent, the naked mole rat. , They reported ... rats protects and alters the activity of the proteasome, ... The factor also protects proteasome function in human, mouse ...
Breaking Biology News(10 mins):Sugar substance 'kills' good HDL cholesterol, new research finds 2Factor in naked mole rat's cells enhances protein integrity 2
... are substantially more different genetically than humans living on ... PLoS Genetics . The study suggests that genomics ... chimpanzee conservation, with the potential to identify the population ... of a sample of bush meat. Common ...
... shapes and sizes. Scientists have discovered simple rules that control leaf ... computer model able to accurately emulate leaf growth from a bud. ... same rate," said lead author Samantha Fox from the John Innes ... like a bud, not flat with a pointed tip." By ...
... the peas please! How often do we hear our children ... Prevention,s Youth Risk Behavior Surveillance System survey of adolescents, only 21% ... vegetables per day. So not very many children are asking ... other methods to get their children to eat their vegetables. ...
Cached Biology News:Nearby chimpanzee populations show much greater genetic diversity than distant human populations 2Nearby chimpanzee populations show much greater genetic diversity than distant human populations 3First model of how buds grow into leaves 2Should we play hide-and-go-seek with our children's vegetables? 2Should we play hide-and-go-seek with our children's vegetables? 3
Rabbit polyclonal to MDM2 (phospho S185) ( Abpromise for all tested applications). entrezGeneID: 4193 SwissProtID: Q00987...
Anti-ADAM-12 (Meltrin Alpha); carboxy terminal end; rabbit host...
Chaps Cell Extract Buffer can be used to lyse cells under nondenaturing conditions and is recommendedfor the preparation of cell lysates to be used with our caspase signaling pathway antibodies...
Mouse monoclonal [1Q17] to TGE Virus peplomer ( Abpromise for all tested applications)....
Biology Products: