Navigation Links
prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification


Powerful high-fidelity RT-PCR systems in two convenient formats

Michael Borns Janice Cline Kris Connors Ronda Allen Holly Hogrefe
Stratagene

Stratagenes prostar RT-PCR systems are powerful systems that permit efficient, high-fidelity amplification of RNA in two convenient formats. The ProSTAR Ultra HF RT-PCR system features pfuturbo DNA polymerase * for amplifying cDNAs with the highest replication fidelity possible using a versatile two-tube format. The ProSTAR HF Single-Tube RT-PCR system combines the sensitivity and accuracy of the TaqPlus Precision DNA polymerase ** blend with the convenience of a one-step, one-tube procedure. These two systems are ideally suited for subcloning, expression, and sequencing of DNA coding regions and for reproducibly detecting and analyzing RNA molecules.

Traditional techniques used to study gene expression include Northern blotting, RNase protection, in situ hybridization, dot blots, and S1 nuclease assays. However, because coupled reverse transcription and PCR amplification (RT-PCR) is an easy, sensitive, and versatile technique, it is the method of choice for analyzing RNA. RT-PCR can be used to determine the presence or absence of a transcript, to quantify expression levels, and to clone rare messages without needing to construct and screen cDNA libraries.

RT-PCR kits differ not only with respect to the RT and PCR enzymes employed, but also with the procedures whereby cDNA synthesis and PCR amplification are coupled. MMLV reverse transcriptase (MMLV-RT), AMV RT, and Tth DNA polymerase are most commonly used to perform cDNA synthesis.1,2,3,4 Second-strand synthesis and DNA amplification can then be carried out using Taq or Tth DNA polymerase,1,2,3,4 or more recently, Taq-based DNA polymerase blends. Applying these ble
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
2. Brilliant Core Reagent Kits for QPCR and QRT-PCR
3. Control RT-PCR Primers for Human Gene Transcripts with Varying Abundance
4. Performance Comparisons of Commercial RT-PCR Systems
5. RT-PCR Primer Sets for Human and Mouse Mismatch Repair Genes
6. RT-PCR Primers for the Study of Apoptosis
7. cMaster RTplusPCR System for increased sensitivity and product length in RT-PCR
8. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
9. Optimized Dengue RT-PCR
10. QuantiTect SYBR Green RT-PCR Kit
11. C. therm. Polymerase One-Step RT-PCR System
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: prostar PCR Systems for Robust High Fidelity RNA Amplification

(Date:1/15/2014)... January 15, 2014 The Pittcon Organizing Committee ... Food Safety Tech , an e-Journal and producer of ... agreement for the second year for the co-location of Food ... The partnership provides that the registration fee to attend the ...
(Date:1/15/2014)... RedBrick Health , a fast-growing leader in consumer ... EmblemHealth , the New York ... the RedBrick Compass health assessment, combined with the RedBrick ... members. EmblemHealth is among the first health plans in ...
(Date:1/15/2014)... 2014 The Microcompetition with Foreign DNA theory ... of these latent viruses is the Epstein Barr Virus (EBV), ... arthritis (RA) is a chronic inflammatory disease that destroys the ... that RA patients have high concentrations of EBV DNA in ...
(Date:1/15/2014)... Biotechnology Company, Limited ("TaiGen") today announced that they have ... pharmaceutical company, to develop and commercialize nemonoxacin (Taigexyn ® ... Turkey and other members of ... for the treatment of bacterial infections including those caused ...
Breaking Biology Technology:Pittcon Announces Second Year Partnership With Food Safety Tech 2Pittcon Announces Second Year Partnership With Food Safety Tech 3EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4
... Inc. (Nasdaq: NVAX ) announced today that it has ... the National Institute of Allergy and Infectious Diseases (NIAID) of ... the NIAID is to support a segment of the Company,s ... vaccine. RSV is the most commonly identified cause of ...
... ... including the life sciences, oil and gas, and chemical industries, Smith provides a successful ... that consistently exceed revenue targets. , ... Chicago, IL (PRWEB) October 13, 2009 -- ClearTrial ( www.cleartrial.com ), ...
... 13 Amsterdam Molecular,Therapeutics (Euronext: AMT), a leader in ... European Medicines Agency has granted Orphan Drug,Designation to AMT,s ... dystrophy. , Orphan Drug Designation ... market exclusivity in Europe following marketing approval for,AMT-080 if ...
Cached Biology Technology:Novavax Awarded NIH Grant for Respiratory Syncytial Virus (RSV) Vaccine Program 2Novavax Awarded NIH Grant for Respiratory Syncytial Virus (RSV) Vaccine Program 3ClearTrial Names Proven Enterprise Software and Life Sciences Sales Leader Craig Smith as Vice President of Sales 2ClearTrial Names Proven Enterprise Software and Life Sciences Sales Leader Craig Smith as Vice President of Sales 3AMT Receives EMEA Orphan Drug Designation for Duchenne Muscular Dystrophy 2AMT Receives EMEA Orphan Drug Designation for Duchenne Muscular Dystrophy 3
(Date:4/17/2014)... in bone marrow need to produce hydrogen sulfide in ... to a new study from the Center for Craniofacial ... of USC. , Professor Songtao Shi, principal investigator on ... by the cells governs the flow of calcium ions. ... that results in osteogenesis, or the creation of new ...
(Date:4/17/2014)... Several parasites and pathogens that devastate honeybees in Europe, ... Africa, but do not appear to be impacting native ... team of researchers., The invasive pests include including ... East African honeybees appear to be resilient to these ... used to control pests in Europe, Asia and the ...
(Date:4/17/2014)... Yokahama IPCC meeting painted a stark warning on the ... has a greenhouse effect 32 times that of carbon ... that humic substances act as fully regenerable electron acceptors ... held in wetlands instead of being released to the ... is disrupted it may enter into a vicious cycle ...
Breaking Biology News(10 mins):East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3Methane climate change risk suggested by proof of redox cycling of humic substances 2
... Boulder, Colo., USA New Geology postings extend ... years; determine erosion rates for exposure of today,s ... pulses; call mudstones "so complicated as to almost ... the oldest rocks on Earth; postulate that biomarkers ...
... rate of molecular evolution between humans and chimps with that of ... at a steady pace in all creatures or if subtle changes ... occur more rapidly in some groups than in others. ... the Royal Society B . The team chose its study ...
... , Jan. 7, 2014 /PRNewswire-iReach/ -- As part of the ... announced the acquisition of MDSL International - a specialist clinical ... Data Management and Statistical services. The financial details of the ... branch of CROS NT, sees the acquisition as an important ...
Cached Biology News:Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 2Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 3Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 4Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 5Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 6Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 7Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 8Geological Society of America's top journal, Geology, begins 2014 with 10 new articles 9Of lice and men (and chimps): Study tracks pace of molecular evolution 2Of lice and men (and chimps): Study tracks pace of molecular evolution 3CROS NT Ltd Announces Acquisition of MDSL International Ltd. 2CROS NT Ltd Announces Acquisition of MDSL International Ltd. 3
Mouse monoclonal [GB3] to Kalinin ( Abpromise for all tested applications). entrezGeneID: 3918 SwissProtID: Q13753...
Rabbit polyclonal to VEZT ( Abpromise for all tested applications). Antigen: Full length human VEZT conjugated to GST Entrez Gene ID: 55591 Swiss Protein ID: Q9HBM0...
AADAC Immunogen: AADAC (NP_001077, 201 a.a. ~ 301 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
Mouse monoclonal [CIa] to Ia Antigen (Biotin) ( Abpromise for all tested applications)....
Biology Products: