Navigation Links
Principles and applications of electrical cell fusion

Producing monoclonal antibodies

Traceable to G. Khler and C. Milstein [4], this method revolutionized the production of antibodies. Primary B lymphocytes, which produce a specific antibody, are fused with tumor cells.

The numerous fusion products are isolated and selected. These single cells are replicated as clones, which is why the term monoclonal is used.

The fusion products, described as hybridoma, stand out for two reasons: they secrete a specific antibody and they can be replicated in culture almost infinitely. This has enabled mass production of specific antibodies.

For these achievements, G. Khler, C. Milstein, and N. K. Jerne were awarded the Nobel Prize for Medicine in 1984.


Cloning mammals


The famous case of Dolly, the cloned sheep [5], would not have been possible without cell fusion. The first step in mammalian cloning is the removal of the nucleus from an oocyte. This enucleated oocyte is then fused with a somatic cell (e.g. from connective tissue). This is carried out by using electrical cell fusion because it is the only method efficient enough to produce the desired result.

The fusion product is implanted into a surrogate mother and is then carried through the full gestation period.

Cloning mammals from embryonic cells was carried out as long ago as the late 1980s [6 -10] and aroused little public interest at the time.

Vaccination against cancer
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Principles and applications of electrical cell fusion
2. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
3. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
4. Suggested applications for reverse transcriptases
5. Self-Drying Vacuum Pumps... the solution for applications producing condensation
6. A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology
7. Production of Hybridomas by Electrofusion
8. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
9. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Principles and applications electrical cell fusion

(Date:12/16/2009)... cell culture supplement? InVitria has what scientists have been asking ... that improves the growth and productivity of CHO cells. Developed ... an animal free, well defined and consistent recombinant CHO supplement. ... ... December 16, 2009 -- Scientists working with CHO cells can ...
(Date:12/15/2009)... one of three Department of Energy (DOE) bio-energy research ... protect its valuable intellectual property. , ... ... 2009 -- Surety, LLC , the leading provider ... that the Joint BioEnergy Institute (JBEI), one of three ...
(Date:12/15/2009)... WILMINGTON, Del. and CHARLOTTESVILLE, Va., Dec. 15 ... ) and the University of Virginia (UVa) ... entered into a strategic research collaboration to ... coronary artery disease (CAD) with a secondary ... (Logo: http://www.newscom.com/cgi-bin/prnh/20091027/PH99766LOGO ) ,, The ...
(Date:12/15/2009)... MANOR, N.Y., Dec. 15 On January ... International Society for Medical Publication Professionals (ISMPP) will ... in London, UK. More than 100 attendees are ... forum for publication professionals, editors, publishers, medical writers ... of medical publications and the impact of guidelines ...
Breaking Biology Technology:Online Video of InVitria's Zap-CHO Helps Scientists Maximize CHO Cell Culture Performance 2Joint BioEnergy Institute Selects Surety's AbsoluteProof to Protect the Integrity and Legally Defend the Authenticity of its Scientific Intellectual Property 2Joint BioEnergy Institute Selects Surety's AbsoluteProof to Protect the Integrity and Legally Defend the Authenticity of its Scientific Intellectual Property 3Joint BioEnergy Institute Selects Surety's AbsoluteProof to Protect the Integrity and Legally Defend the Authenticity of its Scientific Intellectual Property 4Joint BioEnergy Institute Selects Surety's AbsoluteProof to Protect the Integrity and Legally Defend the Authenticity of its Scientific Intellectual Property 5AstraZeneca and the University of Virginia Sign Strategic Research Collaboration to Develop Novel Treatments for Cardiovascular Disease 2AstraZeneca and the University of Virginia Sign Strategic Research Collaboration to Develop Novel Treatments for Cardiovascular Disease 3Announcing ISMPP EU 2010 - the First European Meeting of the International Society for Medical Publication Professionals, Royal College of Physicians, London, UK, January 20, 2010 2
... LAKES, N.J., Nov. 15 Medco Health,Solutions, Inc. (NYSE: ... B.,Snow Jr., and other members of his senior management ... and financial results to,analysts and institutional investors during its ... event, titled "Advancing the,Practice of Pharmacy," is scheduled to ...
... Hoffmann-La Roche Inc. CEO George Abercrombie, Pfizer R&D Head ... & FDA,Commissioner Mark McClellan Headline Agenda at FDA/CMS Summit ... to be a,record year for drug approvals. Unfortunately for ... Food and Drug Administration is on track,to grant marketing ...
... Dr. Brad Thompson, President,and CEO of Oncolytics Biotech ... overview of the company at Toronto CFA Society ... held at the National Club, 303 Bay,Street, Toronto ... Oncolytics is a Calgary-based biotechnology company focused ...
Other Biology Technology:Medco to Webcast Annual Analyst Day Conference 2So Long, Old Friend: The End of the Blockbuster Drug Era? What 2008 Holds for Bringing Drugs to Market 2So Long, Old Friend: The End of the Blockbuster Drug Era? What 2008 Holds for Bringing Drugs to Market 3Media Advisory - Oncolytics Biotech Inc. to Present at Toronto CFA Society 2
(Date:12/16/2009)... an intricate network of interactions. Today,s techniques, however, ... reactions outside the cells. Since molecular concentrations are ... scientists suspect that the kinetics of molecular reactions ... We expected the cellular reaction speed to be ... our novel optical approach showed that depending ...
(Date:12/16/2009)... exposure to pesticides in rivers and basins may ... populations. In addition to the widespread deterioration of ... commonly used pesticides may further inhibit the recovery ... efforts are currently underway to restore Pacific salmon ... says David Baldwin of the National Oceanic and ...
(Date:12/16/2009)... Hill, CA Tom Bowman, an expert in communicating ... Global Change, has developed a series of graphics that ... Climate Change (IPCC) report for public audiences. These new ... they need to make informed decisions about climate change ... as a means to clearly and accurately communicate findings ...
Breaking Biology News(10 mins):Looking for the heartbeat of cellular networks 2Even at sublethal levels, pesticides may slow the recovery of wild salmon populations 2Bowman creates graphic translation of climate change data 2 26 23163 3B4 9 million to develop metamaterials for invisibility cloaks and perfect lenses 14838 1 26 23163 3B4 9 million to develop metamaterials for invisibility cloaks and perfect lenses 14838 2 26 23163 3B4 9 million to develop metamaterials for invisibility cloaks and perfect lenses 14838 3In touch with molecules 14836 1In touch with molecules 14836 2Joslin Diabetes Centers Upgraded Website Offers Improved Access 61361 1
... to move your arms or to walk again. An estimated ... Or imagine being mostly blind, or mostly deaf. Conservatively, tens ... Now imagine being able to stand up and walk, to ... e-mail on a computer, or to see and hear again. ...
... links between the human brain and machines, or ... changing environmental conditions, or computer chips and environmental ... that eliminate currently untreatable conditions such as blindness ... occurring at the same time. Far-fetched? Not so. ...
... model for an agreement on environmental cooperation between Israel and ... Institutes for Desert Research (BIDR) of Ben-Gurion University of the ... Tal, a member of the Swiss Institute for Dryland Research ... Zeit University. , Tal is a long-time environmental activist ...
Other Biology News:Brown to host conference on advances in neurotechnology 2Brown to host conference on advances in neurotechnology 3The coming convergence 2The coming convergence 3Environmental peace treaty between Israel and Palestinians unveiled at Ben-Gurion University 2
Human VEGF R3/Flt-4 Phycoerythrin MAb (Clone 54733)...
Human Legumain Affinity Purified Polyclonal Ab...
Human Cystatin F MAb (Clone 292103)...
... Ac-DEVD-CHO is an inhibitor ... The concentration of inhibitor ... activity must be determined ... Ten micromolar inhibitor is ...
Biology Products: