Navigation Links
Principles and applications of electrical cell fusion

Kurt Lucas, Eppendorf AG

Significance of cell fusion

Electrical cell fusion is an essential step in some of the most innovative methods in modern biology, such as the production of monoclonal antibodies, the cloning of mammals, and vaccination against cancer. Compared to the chemically induced cell fusion via polyethylene glycol (PEG), electrical cell fusion is a highly efficient method.
The process was discovered in 1978 by Zimmermann [1-3], who has initiated ongoing development ever since.

The principle of electrical cell fusion

First, the cells are brought into very close contact via dielectrophoresis. Unlike electrophoresis, in which direct current is applied in order to move molecules, dielectrophoresis uses highfrequency alternating current. In particles such as living cells, dipoles are induced which cause cells to align in such a way that they resemble a string of pearls which are in very close contact with each other.

A very short high-voltage pulse is then applied, which causes permeation of the cell membrane and the subsequent combining of the membranes. The cells then fuse. In order to stabilize the process, alternating voltage is then applied for a brief period.

The resulting formation is described as a heterokaryon because, although the outer cell membrane has fused, two or more cell nuclei are still present. The cell nuclei also fuse at a later point within the cell. In most cases, this results in a drastic reduction in the number of chromosomes in the nucleus.


'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Principles and applications of electrical cell fusion
2. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
3. An Assessment of the Eppendorf twin.tec plate performance in PCR* applications
4. Suggested applications for reverse transcriptases
5. Self-Drying Vacuum Pumps... the solution for applications producing condensation
6. A new wide field-of-view confocal imaging system and its applications in drug discovery and pathology
7. Production of Hybridomas by Electrofusion
8. Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Varian Cary Eclipse
9. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Principles and applications electrical cell fusion

(Date:12/14/2009)... Lionbridge (Nasdaq: LIOX ) ... new, three-year master services agreement with a ... services that serve patients in approximately 100 ... the sole provider for translation of the ... training, human resources, legal, and corporate communications ...
(Date:12/14/2009)... , - Results From ... Rheumatoid Arthritis in Seven Major Industrialized Countries , , ... arthritis say their disease, impacts ... Women living with rheumatoid arthritis find shopping for gifts, ... wrapping presents most, difficult to ...
(Date:12/11/2009)... have discovered a new explanation for differences in the severity ... a certain gene, the more serious the handicap. The genetic ... that it is the amount of copies of the GDI1 ... the American Journal of Human Genetics , and are ... connected to VIB, a life sciences research institute in Flanders, ...
(Date:12/11/2009)... 11 Marrone Bio Innovations (MBI) today ... team. Mr. Chris Hildreth joined MBI as Senior ... for expanding all commercial aspects of the company, ... Kin Sing (Ray) Lam joined MBI as Vice ... development efforts in scaling its broad pipeline of ...
Breaking Biology Technology:Multi-Billion Dollar Biotech Firm Chooses Lionbridge as Exclusive Translation Provider with Three-Year Master Services Agreement 2Multi-Billion Dollar Biotech Firm Chooses Lionbridge as Exclusive Translation Provider with Three-Year Master Services Agreement 3Rheumatoid Arthritis Patients Want Pain-Free Days at Christmas 2Rheumatoid Arthritis Patients Want Pain-Free Days at Christmas 3Rheumatoid Arthritis Patients Want Pain-Free Days at Christmas 4Amount of gene surplus determines severity of mental retardation in males 2Marrone Bio Innovations Announces Two New Executive Appointments 2Marrone Bio Innovations Announces Two New Executive Appointments 3
... Food and Other Packaging Uses, MELBOURNE, Australia, ... DD ) and Plantic Technologies Limited, an Australian ... in the development and sale of renewably sourced ... collaborative development of new, renewably sourced,resins and sheet ...
... Trials Complete for Limited-English Interpretation, TUCSON, ... important for serving limited-English speaking patients in,hospitals ... enhanced with the,development of the industry,s first ... was CyraCom,s invention of its patented,dual-handset phone ...
... Agendia BV, a world leader in,the rapidly evolving field ... US-based Personalized Medicine Coalition (PMC). "The PMC is ... policy on,personalized medicine. We are very pleased to be ... treatment of patients in the future. After we,got both ...
Other Biology Technology:DuPont and Plantic Technologies Announce Starch-Based Bio Materials Collaboration 2DuPont and Plantic Technologies Announce Starch-Based Bio Materials Collaboration 3Industry-First Telephone for Health Care Developed by CyraCom 2Agendia Joins the Personalized Medicine Coalition (PMC) 2
(Date:12/15/2009)... Clinical Laboratory, San Diego, California, has been awarded ... (CAP), based on results of a recent onsite ... advised of this national recognition and congratulated for ... Biotech Inc. GenWay Clinical Laboratory is one of ... Laboratory Accreditation Program, begun in the early 1960s, ...
(Date:12/15/2009)... ., Despite the fact that physical space follows ... to how space is encoded in their language. Some, ... ,left, right, front, back, to talk about spatial relations, ... at all times for all scales: "The spoon is ... by your Northern leg". Whether not only spatial language ...
(Date:12/15/2009)... forward to ultra-low powered or self powered electronic ... This is made possible with power management and ... for power optimisation and higher energy efficiency in ... is the key enabling capability for power management ... advanced analog and mixed signal design capabilities, Singapore,s ...
Breaking Biology News(10 mins):More than a jump to the left 2NTU and EDB launch S$50 million ($36 million) integrated circuit design research center 2NTU and EDB launch S$50 million ($36 million) integrated circuit design research center 3Hanes 28R 29 and Susan G Komen for the Cure 28R 29 Inspire Creativity for a Cause 54718 1Hanes 28R 29 and Susan G Komen for the Cure 28R 29 Inspire Creativity for a Cause 54718 2Hanes 28R 29 and Susan G Komen for the Cure 28R 29 Inspire Creativity for a Cause 54718 3Hanes 28R 29 and Susan G Komen for the Cure 28R 29 Inspire Creativity for a Cause 54718 4Aethlon Medical to Present at Todays Southern California Investor Conference 54714 1Aethlon Medical to Present at Todays Southern California Investor Conference 54714 2Taiwanese Hospital Deploys VoSKY Exchange to Connect Medical Staff to Nursing Trolley via Skype 54711 1Taiwanese Hospital Deploys VoSKY Exchange to Connect Medical Staff to Nursing Trolley via Skype 54711 2
... materials, invented by researchers at the University of Illinois, ... The new materials rely upon embedded, three-dimensional microvascular networks ... manner that a cut in the skin triggers blood ... materials will trigger the flow of healing agent to ...
... Center researchers have uncovered a new mechanism of action ... development of novel regimens in which it is combined ... cancers and solid tumors. , In the new ... for translational research and co-leader of the cancer center’s ...
... it comes to dressing their children, at least among ... States. Researchers at the University of California, Santa Cruz, ... induce different color patterns in their offspring in response ... will wear for the rest of their lives. The ...
Other Biology News:Now, self-healing materials can mimic human skin, healing again and again 2Researchers shed light on mechanism of action used by anti-cancer drug 2Study shows lizard moms dress their children for success 2Study shows lizard moms dress their children for success 3Study shows lizard moms dress their children for success 4
... GrabIt™ Kits are designed for the study ... a pull-down method. Proteins that interact with ... fusion proteins are copurified using affinity resins ... retained on a spin filter. The complexes ...
... Oligo Microarray Kit (V2), 22K is useful for ... response. Each microarray contains content derived from the ... Genomic Research (TIGR) and represents 21,500 genes. ... 1 x 3? glass slides each containing an ...
... Using multicolor analysis in a modern ... of cells that are differentially labeled with ... the dyes typically used for cell labeling ... generated by a flow cytometers filters. Thus, ...
... This CLS number is a ... easily match Cornings product number. ... please order under the old ... customer service for assistance. ID ...
Biology Products: