Navigation Links
pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

PDH mRNA (CMV-Gap) or a scrambled sequence (CMV-Scr). (A) Immunostaining of HeLa cells two days after transfection showing reduction of GAPDH levels in cells transfected with CMV-Gap. Green: GAPDH protein. Blue: DAPI stained nuclei. (B) Relative levels of GAPDH expression compared with HeLa cells not transfected (NT).

Figure 2. Expression of miRNA Using pSilencer 4.1-CMV hygro. pSilencer 4.1-CMV was engineered to express mir-16 or mir-124 pri-miRNA. (A) Schematic representation of the mir-16 and mir-124 transcripts. Each transcript included the predicted hairpin at its center and flanking genomic sequences of various sizes. (B) Two days after transfection into HeLa cells, total RNA was isolated with the mirVana miRNA Isolation Kit and 1 g of total RNA was analyzed on a denaturing agarose gel. A Northern blot was used to detect let-7 miRNA, 5.8S and 5S rRNA while miR-16 and miR-124 miRNA were detected by solution hybridization with the mirVana miRNA Detection Kit. All probes were labeled and purified with the mirVana Probe & Marker Kit. NT = not transfected.


Two days after transfection, total RNA was isolated from the cell cultures using the mirVana miRNA Isolation Kit, which provides quantitative yield of small RNAs, including miRNA, siRNA, tRNA, and 5S/5.8S rRNAs. Analysis of miRNA expression by solution hybridization assay with the mirVana miRNA Detection Kit showed a significant overexpression of miR-16 in cells transfected with the pSilencer 4.1-CMV miR-16 vector (Figure 2B). In
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Selecting siRNA Sequences to Incorporate into the pSilencer Vectors
2. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
3. A Versatile Power Supply for All Electrophoresis Applications
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Versatile Reporter Vectors for Monitoring Viral Transduction
6. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
7. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: pSilencer CMV Versatile Vectors for Expression siRNA miRNA and mRNA

(Date:9/16/2014)... In preparation for VARTECH 2014, 2FA, Inc., ... focused on authentication and SSO announced today that their ... generate a OTP through the presentation of an RFID ... RFID authentication, 2FA,s solutions have leveraged the existence of ... RFID authentication is broadly deployed in healthcare and other ...
(Date:9/15/2014)... 15, 2014   Global Science & Technology, ... products and services for satellite and weather systems, ... Integrated Services (OASIS) from the General Services Administration ... to provide the government best-value solutions for complex ... category in Pool 4. The OASIS SB award ...
(Date:9/15/2014)... downhill fast when a patient has sepsis, a life-threatening ... patient,s blood -- often too fast for antibiotics to ... and developed by a team at Harvard,s Wyss Institute ... doctors treat sepsis. , "Even with the best ... units at least 30 percent of the time," said ...
(Date:9/15/2014)... , Sept. 15, 2014  iVinci Health LLC, the ... Patient Revenue Management system, branded as VisitPay®, has successfully ... announced the launch of a new extension to VisitPay® ... an easy and convenient way for patients to manage ... owed by them and their families. The ...
Breaking Biology Technology:2FA, Inc. Announces RFID-based One-Time Password (OTP) Authentication 2Global Science & Technology, Inc. Awarded GSA OASIS Small Business Contract 2Global Science & Technology, Inc. Awarded GSA OASIS Small Business Contract 3Blood-cleansing biospleen device developed for sepsis therapy 2Blood-cleansing biospleen device developed for sepsis therapy 3Blood-cleansing biospleen device developed for sepsis therapy 4iVinci Health Raises $5M to Fuel National Expansion, Announces Launch of Personal Finance Portal for Healthcare 2iVinci Health Raises $5M to Fuel National Expansion, Announces Launch of Personal Finance Portal for Healthcare 3
... TAMPA, Fla., May 1 MiMedx Group, Inc. ... Wallace, former,President of UPS International, has been appointed ... Commenting on the appointment, Thomas D,Alonzo, Chief ... of Ron,s stature,independence and knowledge to our Board. ...
... May 1 Sidec announces the first,Protein ... PEGS. This Award,is set up to enable ... not yet collaborated with Sidec on a ... groundbreaking technology successfully used in,cancer drug research., ...
... Doctors Changing the Face of ... ... ENTERPRISE (BE),unveils its 2008 report on the best and brightest in medicine. ... lives through the revolutionary treatment of,diseases, from cancer and diabetes to heart ...
Cached Biology Technology:MiMedx Group, Inc. Adds Ronald G. Wallace to Board of Directors 2Sidec Announces the First Protein Tomography(TM) Award at Sidec's Executive Forum at PEGS 2Black Enterprise Unveils America's Leading Doctors 2Black Enterprise Unveils America's Leading Doctors 3
(Date:9/16/2014)... 2014 As open enrollment season approaches, U.S. employees ... their 2015 benefits. According to Aon Hewitt , ... Aon plc (NYSE: AON ), ... role in managing their health care decisions. This increased ... to understand how they can make the most of ...
(Date:9/15/2014)... (September 15, 2014) The so-called central dogma ... proteinhas long provided a simplified explanation for how ... organisms. , In reality, of course, the ... first articulated nearly 60 years ago by Nobel ... structure. For one, there are multiple types of ...
(Date:9/15/2014)... epilepsy drug to a morphine regimen can result in ... combination can reduce the dosage of the opioid needed ... researchers at Indiana University. , The result could bring ... difficult-to-treat condition often felt in the arms and legs ... a huge unmet need for better treatments for neuropathic ...
Breaking Biology News(10 mins):Aon Hewitt Provides Tips to Help Workers Prepare for the Upcoming Open Enrollment Season 2Aon Hewitt Provides Tips to Help Workers Prepare for the Upcoming Open Enrollment Season 3Aon Hewitt Provides Tips to Help Workers Prepare for the Upcoming Open Enrollment Season 4Aon Hewitt Provides Tips to Help Workers Prepare for the Upcoming Open Enrollment Season 5Aon Hewitt Provides Tips to Help Workers Prepare for the Upcoming Open Enrollment Season 6Aon Hewitt Provides Tips to Help Workers Prepare for the Upcoming Open Enrollment Season 7Scientists discover RNA modifications in some unexpected places 2IU study: Combining epilepsy drug, morphine can result in less pain, lower opioid doses 2
... A new research report published online in The FASEB Journal ... sugar and high fat diets have babies who are likely ... which used rats, this happens because the high fat and ... reward pathway, altering food preferences. Not only does this offer ...
... Minnesota researchers are a key step closer to making renewable ... by a $2.2 million United States Department of Energy grant. ... January, made the critical step by figuring out how to ... bacteria into ketones, which can be cracked to make hydrocarbon ...
... has been used to treat heroin addicts for nearly ... possible harmful effects from prolonged use. New research from ... affects the brain and impairs the attention of experimental ... and morphine are known to weaken intellectual functions such ...
Cached Biology News:'Junk food' moms have 'junk food' babies 2U of M researchers close in on technology for making renewable petroleum 2Long-term methadone treatment can affect the brain 2
... Hollandes is a fixative useful for routine ... It is stable and will decalcify small ... Hollande's can be stained successfully with most ... solution stabilizes red blood cell membranes and ...
... The gold standard for automated ... of bioanalytical applications • Based on ... • Automated accessories designed for bioresearch ... light • Versatility, reliability and ease-of-use ...
... In Vitro Osteogenesis Assay Kit contains all ... a mature osteoblastic lineage, as determined by ... provides all the necessary reagents and a ... plate reader. This product is useful for ...
... Acuity is the complete enterprise microarray informatics platform ... analysis. Buy Acuity once and use it forever ... Tools for Quality Acuity has all the ... from your data, from quality control statistics and ...
Biology Products: