Navigation Links
pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA

PDH mRNA (CMV-Gap) or a scrambled sequence (CMV-Scr). (A) Immunostaining of HeLa cells two days after transfection showing reduction of GAPDH levels in cells transfected with CMV-Gap. Green: GAPDH protein. Blue: DAPI stained nuclei. (B) Relative levels of GAPDH expression compared with HeLa cells not transfected (NT).

Figure 2. Expression of miRNA Using pSilencer 4.1-CMV hygro. pSilencer 4.1-CMV was engineered to express mir-16 or mir-124 pri-miRNA. (A) Schematic representation of the mir-16 and mir-124 transcripts. Each transcript included the predicted hairpin at its center and flanking genomic sequences of various sizes. (B) Two days after transfection into HeLa cells, total RNA was isolated with the mirVana miRNA Isolation Kit and 1 g of total RNA was analyzed on a denaturing agarose gel. A Northern blot was used to detect let-7 miRNA, 5.8S and 5S rRNA while miR-16 and miR-124 miRNA were detected by solution hybridization with the mirVana miRNA Detection Kit. All probes were labeled and purified with the mirVana Probe & Marker Kit. NT = not transfected.


Two days after transfection, total RNA was isolated from the cell cultures using the mirVana miRNA Isolation Kit, which provides quantitative yield of small RNAs, including miRNA, siRNA, tRNA, and 5S/5.8S rRNAs. Analysis of miRNA expression by solution hybridization assay with the mirVana miRNA Detection Kit showed a significant overexpression of miR-16 in cells transfected with the pSilencer 4.1-CMV miR-16 vector (Figure 2B). In
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Selecting siRNA Sequences to Incorporate into the pSilencer Vectors
2. Your Data: Use of pSilencer 1.0-U6 siRNA Expression Vector in the Analysis of Synaptotagmin Location and Function
3. A Versatile Power Supply for All Electrophoresis Applications
4. Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in Mammalian Cells
5. Versatile Reporter Vectors for Monitoring Viral Transduction
6. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
7. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
8. Epitope-Tagging Vectors for Functional Analysis in Yeast
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Efficiently Insert Unique Restriction Sites into Plasmid Vectors
11. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: pSilencer CMV Versatile Vectors for Expression siRNA miRNA and mRNA

(Date:7/24/2015)... , July 24, 2015 Orexigen Therapeutics, ... financial results for the second quarter 2015 on Thursday, ... Orexigen will provide a business update and discuss the ... at 8:00 a.m. Eastern Time (5:00 a.m. Pacific Time). ... phone by calling (800) 447-0521 (domestic) or (847) 413-3238 ...
(Date:7/24/2015)... , ... July 24, 2015 ... ... class-leading frozen sample management solution, indispensable to any modern scientific or pharmaceutical ... has a user-friendly interface. Users know precisely where a sample is located ...
(Date:7/23/2015)... ... July 23, 2015 , ... One hundred years ago ... 1915 National Model Aeroplane Competition, with hopes of winning the Henry S. Villard Trophy. ... centennial. , Participants will have the opportunity to build a Free Flight fantasy club ...
(Date:7/23/2015)... /PRNewswire/ - Portage Biotech Inc. ("Portage") (OTC Market: PTGEF, ... Company Limited (Biohaven), are pleased to announce that ... Application with the U.S. Food and Drug Administration ... holds 54% equity interest in Biohaven, a private ... agent and Biohaven has entered into an exclusive ...
Breaking Biology Technology:RURO, Inc. Partners with Integrated Systems Engineering to Provide Comprehensive LIMS Software to Biobanks 2RURO, Inc. Partners with Integrated Systems Engineering to Provide Comprehensive LIMS Software to Biobanks 3RURO, Inc. Partners with Integrated Systems Engineering to Provide Comprehensive LIMS Software to Biobanks 4Academy of Model Aeronautics to Start Online Fantasy Flight Competition 2Portage's Biohaven Announces IND Filing for BHV-0223 for Phase 1 Clinical Testing 2Portage's Biohaven Announces IND Filing for BHV-0223 for Phase 1 Clinical Testing 3Portage's Biohaven Announces IND Filing for BHV-0223 for Phase 1 Clinical Testing 4
... ATSI ), manufacturer and marketer of ... formation of the ATS Medical,Cryoablation Surgical Advisory Board. ... ) , This new ... position in surgical ablation. The board is comprised ...
... - Tamiflu Remains Fully Effective Against 94 ... Latest surveillance data coming through the European ... clinicians indicates that,the influenza A(H3N2) virus is the predominant ... data published by ECDC Roche confirms that the oral,antiviral ...
... 19 Environmental Tectonics Corporation,s (Amex: ... today announced the sale of two BARA-MED(R) XD ... Hospital in Allentown, PA. St. Luke,s Hospital - ... Hospital & Health Network, a nationally recognized, regional, ...
Cached Biology Technology:ATS Medical Announces Formation of Cryoablation Surgical Advisory Board 2ATS Medical Announces Formation of Cryoablation Surgical Advisory Board 3Seasonal Influenza Activity Increasing Across Europe - H3N2 Dominant Strain 2Seasonal Influenza Activity Increasing Across Europe - H3N2 Dominant Strain 3Seasonal Influenza Activity Increasing Across Europe - H3N2 Dominant Strain 4Environmental Tectonics Corporation Announces the Sale of Two BARA-MED(R) XD Monoplace Hyperbaric Chambers 2Environmental Tectonics Corporation Announces the Sale of Two BARA-MED(R) XD Monoplace Hyperbaric Chambers 3Environmental Tectonics Corporation Announces the Sale of Two BARA-MED(R) XD Monoplace Hyperbaric Chambers 4
(Date:7/2/2015)... , July 2, 2015 Fingerprint ... FPC1025, FPC1035, FPC1145 and FPC1155 from the distributor World Peace ... of FPC,s distributors in Asia . Deliveries ... The sensors will be used by smartphone manufacturers in ... is included in the communicated revenue guidance of approximately 2,200 ...
(Date:6/30/2015)... 30, 2015 Genisphere announced ... to help further develop Genisphere,s therapeutics capabilities and portfolio. ... having spent much of the last 25 years in ... Bioscience, and Johnson & Johnson. Tom started his career ... Biotech Corporate Finance. He graduated from Dartmouth College with ...
(Date:6/26/2015)... ATL Technology, LLC, a top provider of electromechanical ... use solutions, headquartered in Springville, Utah , ... California corporation with locations in Santa ... Rica ). This acquisition will incorporate MedConx,s ... ATL Technology,s existing facility in Costa Rica ...
Breaking Biology News(10 mins):FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2Genisphere Appoints New CEO, Thomas Bliss 2Genisphere Appoints New CEO, Thomas Bliss 3ATL Technology Announces Acquisition of MedConx, Inc. and Expanded Global Footprint 2
... a critical means of communication for many deaf people, but ... b) can be nearly impossible to distinguish by sight alone. ... can be felt by the skin, can help overcome that ... from lip reading. Researchers in MIT,s Sensory Communication Group ...
... garden with no more technology than Darwin had in his ... it too? A Journal of Biology article argues ... viewpoint that prevented him from interpreting the evidence that was ... Darwin,s commitment to quantitative variation as the raw material of ...
... It has been estimated that in the United States, ... livestock producers millions of dollars a year and ... Consequently, a University of Miami (UM) scientist and researchers ... developed the first weather system advisory designed to improve ...
Cached Biology News:MIT: Using touch to help deaf people 2Why didn't Darwin discover Mendel's laws? 2How cold is too cold for newborn calves? 2