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pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins

e ecdysone-inducible vectors need to be delivered by transfection. At this writing, several ecdysone-inducible retroviral constructions are being evaluated for efficient delivery of inducible expression cassettes that allow fine control of expression and high-level induction of the gene of interest. The use of a two-virus system for delivery of the edysone-inducible expression system will further expand the range of cell types in which ecdysone-regulated expression can be achieved, while further reducing the time, cost, and labor of screening for clones that show optimal expression characteristics.

REFERENCES

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  4. Miller, A.D. (1997) In Retroviruses (Eds., Coffin, J.M., Hughes, S.H., and Varmus, H.E.) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. pp. 437-473.

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