Navigation Links
pFB-ERV: Retroviral Delivery of the Ecdysone Receptor Proteins


Faster, easier screening for difficult transfections when using the complete control kit

Peter Vaillancourt Katherine A. Felts
Stratagene

We describe the vector pFB-ERV, an MMLV-based replication-defective retroviral vector for delivery of the ecdysone receptor proteins RXR and VgEcR. In stable cell lines infected with an estimated single copy of the receptor cassette, induction ratios of greater than 200-fold are attainable in transient reporter transfection assays. The neo-resistance marker is expressed as the third open reading frame (ORF) in a tricistronic CMV expression cassette (the two receptors comprise the first and second ORFs); cell lines harboring single-copy integrants are resistant to as high as 1 mg/ml G418. The CMV promoter ### is flanked by unique restriction sites and, thus, can be replaced with a cell-type specific promoter of interest.

DNA vector-based systems that allow precise control of gene expression in vivo have become invaluable for the study of gene function in a variety of organisms, particularly when applied to the study of developmental and other biological processes for which the timing or dosage of gene expression is critical to gene function. Such systems have also been successfully used to overexpress toxic or disease-causing genes, to induce gene targeting, and to express antisense RNA. Inducible systems are currently being used by pharmaceutical companies to facilitate screening for inhibitors of clinically relevant biological pathways, and potential applications for gene therapy are being explored.1

Stratagenes complete control inducible system is based on the insect molting hormone ecdysone, which can stimulate transcriptional activation in mammalian cells harboring the ecdysone receptor protein from Drosophila melanogaster.2,3 The ecdysone-inducibl
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
2. High-Titer Retroviral Vectors for Gene Delivery
3. A New Way to Assess siRNA Delivery Efficiency
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. Enhanced siRNA Delivery and Long-term Gene Silencing
6. High Throughput siRNA Delivery In Vitro: From Cell Lines to Primary Cells
7. Delivery of pCMV-S DNA Using the Helios Gene Gun System Is Superior to Intramuscular Injection in Balb/c Mice
8. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
9. Detecting Gastrin-Releasing Peptide Receptor by in situ PCR on Archived Tissue
10. Gene-Specific Primers for NMDA Receptor Subunits
11. TKB1 Cells Identify Receptor Tyrosine Kinase Interacting Proteins
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: pFB ERV Retroviral Delivery the Ecdysone Receptor Proteins

(Date:12/22/2014)... HILL, N.C. , Dec. 22, 2014 /PRNewswire/ ... today announced that it has signed a letter ... company that has developed and patented a nanotechnology-based ... medical diagnostics products that enable rapid on-site collection ... alcohol use/abuse and health issues in an immediate, ...
(Date:12/22/2014)... ITRA Global, one of the largest organizations dedicated ... has further expanded its global reach with the affiliation ... Australia, reports Mylinda Vick, CCIM, Chairman of the ITRA ... ACORPP (Australian Corporate Property and Projects) , is a ... to a wide range of clients in the private ...
(Date:12/19/2014)... BioPlus Specialty Pharmacy (BioPlus), one of the nation’s ... Pharm.D. to the newly created position of Vice President ... Dr. Maroulis will continue to manage the entire specialty ... pharmacies as the company expands. Dr. Maroulis has been ... has served in many different capacities in the pharmacy, ...
(Date:12/19/2014)... , Dec. 19, 2014 Decision Resources Group ... grow at a 12 percent compound annual growth rate ... the aging population and the increasing adoption of dental ... will also spur demand for dental biomaterials ... implant and periodontal treatments. Other key findings ...
Breaking Biology Technology:ALNE Announces Intention To Acquire BioTechPharma 2ITRA Global Continues International Expansion in Perth and Brisbane, Australia 2BioPlus Specialty Pharmacy Promotes Dr. Nick Maroulis to Vice President of Specialty Pharmacy Services 2Latin American Dental Biomaterial Market Will Expand at a 12 Percent Annual Growth Rate Through 2023 2Latin American Dental Biomaterial Market Will Expand at a 12 Percent Annual Growth Rate Through 2023 3
... facility to support clinical development for ... lead drug candidate and other molecules, ... radiopharmaceutical company that designs, develops and,manufactures products to detect, ... completed commissioning its in-house manufacturing,capability to meet clinical trial ...
... (OTC Bulletin Board: YGYB) ("Yongye" or the "Company"),a leading ... in the People,s Republic of China, today announced its ... Second Quarter 2008 Highlights -- Revenue was ... quarter of 2007, and an ...
... 15 The backpack is a back-to-school,staple. In ... fashion accessory as,well. However, backpacks are a leading ... children and adolescents. As students head back to ... preventing,unnecessary backpack pain and injuries., The MCA ...
Cached Biology Technology:Cellectar, Inc. Begins GMP Production 2Yongye Biotechnology International Announces Second Quarter Results 2Yongye Biotechnology International Announces Second Quarter Results 3Yongye Biotechnology International Announces Second Quarter Results 4Yongye Biotechnology International Announces Second Quarter Results 5Yongye Biotechnology International Announces Second Quarter Results 6Yongye Biotechnology International Announces Second Quarter Results 7Yongye Biotechnology International Announces Second Quarter Results 8Yongye Biotechnology International Announces Second Quarter Results 9Yongye Biotechnology International Announces Second Quarter Results 10Yongye Biotechnology International Announces Second Quarter Results 11Minnesota Chiropractic Association Offers Backpack Safety Checklist 2
(Date:12/10/2014)... , December 9, 2014 ... ITN,s BCARD Platform   ... enterprise meeting scheduling solutions for business-to-business (B2B) events, ... world leader in mobile near-field communication (NFC), Bluetooth ...      (Logo: http://photos.prnewswire.com/prnh/20140612/691055) , Jifflenow ...
(Date:12/5/2014)...  Tute Genomics, a leader in cloud-based solutions for ... funding led by UK-based Eurovestech. Peak Ventures and a ... round. "We are at a pivotal time ... and seeks new approaches for the diagnosis, treatment and ... MBA, and CEO of Tute Genomics. "One of the ...
(Date:12/3/2014)...   Marvin Test Solutions , a trusted ... military, aerospace, and manufacturing organizations, today announced the ... TS-900 PXI semiconductor test platform . The TS-960 ... to customers at a fraction of the footprint ... "Our semiconductor customers asked for ...
Breaking Biology News(10 mins):Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 2Jifflenow And ITN International Bring Cutting-Edge Badge Scanning Technology To B2B Events 3Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3
... Romeo Casabona is Director of the Interuniversity Professorship in Law ... of the Basque Country. The Professorship is largely made up ... in the fields of molecular biology, medicine and even specialists ... Humane Genome at the Universities of Deusto and the Basque ...
... , This release is available in German ... less than half accumulate in the atmosphere where it contributes ... and terrestrial ecosystems such as forests, grasslands and peat-lands. Stimulating ... one of the main, immediately available ways of reducing climate ...
... deletion can increase the likelihood of immune complications following ... in the November 22 advance online issue of ... missing from the donor,s genome but present in the ... serious side-effect known as graft-versus-host disease, in which immune ...
Cached Biology News:Research and legislation should go hand in hand, as much as possible 2Intensive land management leaves Europe without carbon sinks 2Gene mismatch influences success of bone marrow transplants 2Gene mismatch influences success of bone marrow transplants 3Gene mismatch influences success of bone marrow transplants 4
Kinase Buffer can be used to assay protein kinase activity...
Goat polyclonal to SPFH2 ( Abpromise for all tested applications). Antigen: Synthetic peptide: EDEPLETATKEN, corresponding to C terminal amino acids 328-339 of Human SPFH2 Entrez Gene ID: 11...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
alpha/beta-Tubulin Antibody Ship: Hot Store: -20 C...
Biology Products: