( For miRNA purification from formalin...)miRNeasy Mini Kit and miRNeasy 96 Kit

For miRNA purification from formalin-fixed, paraffin-embedded (FFPE) tissue sections (using the RNeasy FFPE Kit), a supplementary protocol is available at www.qiagen.com/miRNA .

Effective miRNA purification from animal tissues and cells
The miRNeasy Mini Kit and the miRNeasy 96 Kit efficiently purify RNA from animal tissues and cells, even when low amounts of starting material are used. In the results shown in Figure 1, C_T values increased linearly when RNA was purified from decreasing amounts of cells or tissue, indicating effective miRNA purification from a wide range of amounts of starting material.

RNA can be purified from a variety of tissues and cells, including difficult-to-lyse tissues (Figure 2).

Purification of total RNA including miRNA allows direct comparison of miRNA expression levels with those of housekeeping reference genes or any other mRNA of interest (Figure 2).

Efficient enrichment of miRNA
For some sensitive downstream applications, an miRNA-enriched fraction without larger RNA may be required. miRNeasy Kits enable efficient enrichment of RNA down to approximately 18 nt in size. In the data shown in Figure 3, the presence of very small RNAs was clearly visible after purification using the miRNeasy Kit, in contrast to samples prepared using an alternative kit.

High-purity RNA
RNA prepared using miRNeasy Kits is highly pure and ready for use in sensitive downstream applications.

miRNeasy procedures eliminate the possibility of con
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