Navigation Links
miRNA Expression in White Blood Cells

ed from the fractionated white blood cells was quantitated by UV absorbance and analyzed by ethidium bromide staining on a 15% denaturing polyacrylamide gel. RNA yields ranged from about 20-40 g per 10 ml blood sample. No obvious degradation of the ribosomal RNA and tRNA bands was observed. Four representative samples are presented in Figure 2.

Figure 2. Analysis of miRNA Expression in White Blood Cells. Leukocytes were collected from 10 ml EDTA-anticoagulated blood samples from four different donors, and total RNA was isolated with the mirVana miRNA Isolation Kit. Samples were analyzed as described in Figure 1 except that a probe specific for miR-22 miRNA was used in the solution hybridization assay.


The RNA samples were then analyzed for the presence of several small RNAs using Northern blot or the mirVana miRNA Detection Kit. Interestingly, several miRNAs--let-7, miR-16, and miR-22--were detected in total RNA from white blood cells (Figure 2). miR-16 was the most abundant miRNA in all of the 14 samples. A direct comparison of total RNA samples from other human tissues showed that miR-16 is expressed at a higher level in white blood cells than in any other tissues we have examined (data not shown). While different expression levels were observed across donors for the few miRNA tested, there was no clear donor-specific difference in the global miRNA expression pattern. Experiments are in progress to determine whether other miRNAs may have donor-specific expression patterns and whether these patterns
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform
2. Detecting miRNA & siRNA
3. Isolate It All: siRNA miRNA Total RNA Native Protein
4. Get the miRNA OutPlant Applications
5. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
6. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
7. Precursor miRNAs for Successful miRNA Functional Studies
8. Prepare siRNA and miRNA Probes in Just 1 Hour
9. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
10. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: miRNA Expression White Blood Cells

(Date:7/24/2015)... Diego, CA (PRWEB) , ... July 24, 2015 , ... ... & Referral Center in support of stem cell therapy training and clinical opportunities. , ... this new opportunity to veterinarians in the San Diego area. Stem cell therapy is ...
(Date:7/24/2015)... 2015  Champions Oncology (OTC-BB: CSBR), a company engaged ... to personalize the development and use of oncology drugs, ... results for the fourth quarter and fiscal year quarter ... before market open. The company will host ... at 9:00 a.m. EDT (6:00 a.m. PDT). To participate ...
(Date:7/24/2015)... , July 24, 2015 Origin Agritech Limited (NASDAQ ... crop seeds in China , today announced ... fiscal year 2015 ended June 30, 2015, before the market ... will host a teleconference on August 5th, 2015, at 9:00 ... time to discuss the results. To participate in the call, ...
(Date:7/24/2015)... China , July 24, 2015 ... manufacturing platform , Expands product offering for chronic ... unmet medical needs in diabetes-related complications and dermatology ... 3SBio Inc., (HKEX:1530) ("3SBio" or ,the Company"), a leading ... researching, developing, manufacturing and marketing biopharmaceutical products, today announced ...
Breaking Biology Technology:VetStem Biopharma, proudly announces a new long term partnership with VCA Emergency Animal Hospital & Referral Center. 2VetStem Biopharma, proudly announces a new long term partnership with VCA Emergency Animal Hospital & Referral Center. 3Champions Oncology to Announce Fourth Quarter Financial Results on Tuesday, July 28 2Origin Agritech Limited to Announce Third Quarter Fiscal 2015 Unaudited Financial Results on August 5th, 2015 2Origin Agritech Limited to Announce Third Quarter Fiscal 2015 Unaudited Financial Results on August 5th, 2015 33SBio Acquires Zhejiang Wansheng Pharmaceutical 23SBio Acquires Zhejiang Wansheng Pharmaceutical 33SBio Acquires Zhejiang Wansheng Pharmaceutical 4
... ( http://www.amdl.com ),AMDL, Inc. (Amex: ADL ), ... China and the US, today announced that the,Company will ... New,York City on Tuesday, October 7, 2008 at 10:30 ... Hyatt New York Hotel in the Morosco Room., ...
... Proceeds to Support R&D Efforts and Clinical ... BETHESDA, Md., Oct. 3 Micromet, Inc. ... company developing,novel, proprietary antibodies for the treatment ... that it has closed its previously,announced $40 ...
... of Aberdeen and Rothamsted Research for releasing natural insect repellents that ... technology is set to revolutionise how people protect buildings from unwanted ... ... (PRWEB) October 3, 2008 -- Atrium Innovation Ltd has developed a ...
Cached Biology Technology:AMDL, Inc. to Present at Maxim Group Growth Conference October 7, 2008 2AMDL, Inc. to Present at Maxim Group Growth Conference October 7, 2008 3Micromet Closes $40 Million Private Equity Placement 2Micromet Closes $40 Million Private Equity Placement 3Micromet Closes $40 Million Private Equity Placement 4New Innovation Set to Revolutionise Spray Technology - Used to Protect Against the Scottish Biting Midge & Help Fight MRSA in the NHS 2New Innovation Set to Revolutionise Spray Technology - Used to Protect Against the Scottish Biting Midge & Help Fight MRSA in the NHS 3New Innovation Set to Revolutionise Spray Technology - Used to Protect Against the Scottish Biting Midge & Help Fight MRSA in the NHS 4
(Date:7/7/2015)... OXFORD, Conn. , July 7, 2015 NXT-ID, Inc. ... company focused on the growing mobile commerce market announces a revised ... airing this week. The commercial will air on CNBC in ... Los Angeles and San Francisco ... The commercial focuses on Wocket,s ability to replace all the ...
(Date:6/30/2015)... -- Genisphere announced Tom Bliss ... develop Genisphere,s therapeutics capabilities and portfolio. Mr. Bliss comes ... of the last 25 years in leadership positions at ... & Johnson. Tom started his career in Investment Banking, ... He graduated from Dartmouth College with a BA in ...
(Date:6/25/2015)... 25, 2015  TAKE Solutions Ltd., a global business ... United States Patent and Trademark Office (USPTO) for its ... TAKE Solutions, Clinical Accelerators to reduce the time taken ... to standardization without the accelerators), thus reducing time to ... At the heart of the patented Clinical Data ...
Breaking Biology News(10 mins):Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 2Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 3Genisphere Appoints New CEO, Thomas Bliss 2Genisphere Appoints New CEO, Thomas Bliss 3TAKE Solutions Awarded Patent By USPTO 2
... the fields of social, biological and medical science will ... discuss how they can cooperate to improve our understanding ... how our genetic inheritance is expressed (epigenetics). This collaboration ... changes have for such key social policy issues as ...
... pharmaceutical companies and four research institutions, working with the ... partnership that aims to speed the discovery of essential ... known as the TB Drug Accelerator (TBDA), will target ... early-stage research. The long-term goal of the TBDA is ...
... is home to a teeming ecosystem of microbes that is ... while the gut microbiota is interacting with our body, they ... study published online in Genome Research , ... set of viruses associated with gut microbiota in global populations. ...
Cached Biology News:World experts meet in Edinburgh to consider how life experiences impact on our genes 27 pharmaceutical companies join academic researchers to speed TB drug discovery 2Gut microbes battle a common set of viruses shared by global populations 2
...
... Clone/PAD: 3B8A10. ... to the C-terminal region ... Specificity: Specific for the ... Reactivity: Human (positive control: ...
Negative Control, eluted and packaged aseptically in 20l of 10mM Tris-HCL, pH 8.0, 1 mM EDTA, 5 years at either 4°C or -20°C...
...
Biology Products: