Navigation Links
miRNA Expression in White Blood Cells

of total RNA.

An example of small RNA analysis using these tools is shown in Figure 1. The data show that the same amount of 5.8S and 5S rRNA was recovered from mouse brain, kidney, liver and thymus. In contrast, significant variations in miRNA levels were observed in these tissues. For example, let-7 was more abundant in brain and kidney, and miR-16 was highly expressed in thymus only. As expected, based on published reports [1, 2], miR-124 was detected only in the brain sample.

Figure 1. Analysis of miRNA Expression Across Mouse Tissues. Total RNA was isolated from ~100 mg of the indicated tissues with the mirVana miRNA Isolation Kit. Purified RNA (1 g) was analyzed on a 15% denaturing polyacrylamide gel stained with ethidium bromide (left panel). let-7 miRNA, and 5.8S and 5S rRNA were detected by Northern blot. miR-16 and miR-124 miRNA were detected by solution hybridization with the mirVana miRNA Detection Kit. All probes were labeled and purified with the mirVana Probe & Marker Kit.


Expression of miRNA in Blood Cells
Although miRNAs have been found in all mammalian tissues examined so far, there is little if any published information about miRNA expression in blood. This may be due to the inherent difficulty in isolating high quality RNA from human blood. To examine the efficiency of the mirVana system for use in analysis of miRNAs in white blood cells, blood was collected from 14 healthy volunteers. Leukocytes were fractionated from whole blood and the total RNA population, including miRNA, was extracted using the mirVana miRNA Isolation Kit. The RNA recover
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform
2. Detecting miRNA & siRNA
3. Isolate It All: siRNA miRNA Total RNA Native Protein
4. Get the miRNA OutPlant Applications
5. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
6. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
7. Precursor miRNAs for Successful miRNA Functional Studies
8. Prepare siRNA and miRNA Probes in Just 1 Hour
9. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
10. An Epitope Tagging Vector for Gene Expression in Mammalian Cells
11. Antibodies for Studying NMDA Receptor Protein Expression and Synapse-Specific Immunolabeling
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: miRNA Expression White Blood Cells

(Date:5/21/2013)... Miami, FL (PRWEB) May 21, 2013 ... and breakouts as contributor to acne. Recently physicians from ... effects of mental stress on the skin, and suggested ... to help reduce the chemicals produced from stress that ... aside from the negative feelings acne brings about, stress ...
(Date:5/21/2013)... BALTIMORE , May 21, 2013  Personal ... will use its proprietary methodologies and expertise in ... novel kinase targets in collaboration with Blueprint Medicines. ... aimed at identifying genomic alterations in tumors, and ... selective kinase inhibitors for a genetically defined patient ...
(Date:5/21/2013)... (PRWEB) May 21, 2013 Inflamax ... of the ITN to design the study, which ... two key clinical models used in the study ... and Nasal Allergen Challenge (NAC) models. Although both ... numerous publications, to date no direct comparison between ...
(Date:5/21/2013)... May 21, 2013 PathoGenetix, Inc., a ... strain typing, announced today that it has successfully identified ... coli strains obtained from the Centers for Disease Control ... (GSS™) technology. The findings are detailed in a ... American Society for Microbiology in Denver on Monday. , ...
Breaking Biology Technology:Adult Acne Treatment, Probiotic Action Explains How Hypnotherapy and Probiotics May Cure Various Skin Conditions 2Personal Genome Diagnostics And Blueprint Medicines Form Collaboration to Identify Novel Kinase Targets 2Personal Genome Diagnostics And Blueprint Medicines Form Collaboration to Identify Novel Kinase Targets 3Inflamax Research selected by the Immune Tolerance Network and the National Institutes of Health to conduct a landmark clinical study on the underlying mechanisms of allergic inflammation. 2New Genotyping System Identifies Pathogenic E. coli Outbreak Strains 2New Genotyping System Identifies Pathogenic E. coli Outbreak Strains 3
... Wis. - Hartford-based api Software, Inc . has ... to four hospitals and health systems, for whom it may ... designed the bundled system so that parts of the bundle ... objective in an era of severe nursing shortages. , ...
... Supreme Court in eBay Inc. v. Mercexchange LLC, held ... be granted an injunction must be determined under the ... (2) inadequacy of remedies at law; (3) balancing of ... the Federal Circuit's approach that an injunction should be ...
... Madison, Wis. - Gov. Jim Doyle has signed ... eliminate some of the bureaucratic obstacles that stand in ... said the law would help researchers more quickly turn ... law requires a lengthy, bureaucratic review whenever UW faculty ...
Cached Biology Technology:Medical software may be a retention tool 2Supreme Court decision in eBay case could affect value of patents 2
(Date:5/22/2013)... May 22, 2013. Plastic additives known as phthalates ... everywhere: They turn up in flooring, plastic cups, ... the Centers for Disease Control and Preventionthe bodies ... have come under increasing scrutiny. A growing collection ... can leech from packaging and mix with food) ...
(Date:5/21/2013)... analysis shows that the nation,s land and water resources ... produce up to 25 billion gallons of algae-based fuel ... country,s yearly needs. , The findings come from an ... needed to grow significant amounts of algae in large, ... the May 7 issue of Environmental Science and ...
(Date:5/21/2013)... of all types become most deadly when they metastasize ... reached this stage, it becomes very difficult for doctors ... develop. Now, researchers at the University of Missouri have ... lymphoma tumor cells wherever they may be in the ... the MU College of Veterinary Medicine, says being able ...
Breaking Biology News(10 mins):Study links chemicals widely found in plastics and processed food to elevated blood pressure in children and teens 2Going green: Nation equipped to grow serious amounts of pond scum for fuel 2Going green: Nation equipped to grow serious amounts of pond scum for fuel 3Going green: Nation equipped to grow serious amounts of pond scum for fuel 4MU researchers develop radioactive nanoparticles that target cancer cells 2
... Internationally respected scientists will gather next week for a ... a mind and to emphasize the need for $4 billion ... years. Presented by Great Ape Trust of Iowa, Decade ... recognized research pioneers from a variety of disciplines May 7-9 ...
... of a good thing could be harmful to the ... to kill harmful bacteria and, recently, have used this ... a University of Missouri researcher has found that silver ... to remove ammonia from wastewater treatment systems. The study ...
... YORK (APRIL 29, 2008) Bison can repopulate large ... years provided a series of conservation and restoration measures ... species by the Wildlife Conservation Society and other groups. ... of conservationists, scientists, ranchers, and Native Americans/First Nations peoples, ...
Cached Biology News:Decade of the Mind symposium in Iowa to emphasize need for $4 billion in federal support 2Decade of the Mind symposium in Iowa to emphasize need for $4 billion in federal support 3Decade of the Mind symposium in Iowa to emphasize need for $4 billion in federal support 4Too much technology may be killing beneficial bacteria 2Bison can thrive again, study says 2
Human GATA-2 Affinity Purified Polyclonal Ab...
...
... priced, full ring small animal imaging PET ... microPET R4 low retail price, it outperfoms ... count rate performance, energy resolution, timing resolution, ... small animal PET (Positron Emission Tomography) imaging ...
... versatile liquid handling platforms and flexible robotic ... of life science applications.The Freedom EVO platform ... 100, 150 and 200 cm), giving all ... will advance with their application needs. ...
Biology Products: