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miRNA Expression in White Blood Cells

of total RNA.

An example of small RNA analysis using these tools is shown in Figure 1. The data show that the same amount of 5.8S and 5S rRNA was recovered from mouse brain, kidney, liver and thymus. In contrast, significant variations in miRNA levels were observed in these tissues. For example, let-7 was more abundant in brain and kidney, and miR-16 was highly expressed in thymus only. As expected, based on published reports [1, 2], miR-124 was detected only in the brain sample.

Figure 1. Analysis of miRNA Expression Across Mouse Tissues. Total RNA was isolated from ~100 mg of the indicated tissues with the mirVana miRNA Isolation Kit. Purified RNA (1 g) was analyzed on a 15% denaturing polyacrylamide gel stained with ethidium bromide (left panel). let-7 miRNA, and 5.8S and 5S rRNA were detected by Northern blot. miR-16 and miR-124 miRNA were detected by solution hybridization with the mirVana miRNA Detection Kit. All probes were labeled and purified with the mirVana Probe & Marker Kit.


Expression of miRNA in Blood Cells
Although miRNAs have been found in all mammalian tissues examined so far, there is little if any published information about miRNA expression in blood. This may be due to the inherent difficulty in isolating high quality RNA from human blood. To examine the efficiency of the mirVana system for use in analysis of miRNAs in white blood cells, blood was collected from 14 healthy volunteers. Leukocytes were fractionated from whole blood and the total RNA population, including miRNA, was extracted using the mirVana miRNA Isolation Kit. The RNA recover
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